breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorspgi-4-3_S11_L001_R1_001.good.fq1,378,613307,263,956100.0%222.9 bases239 bases98.8%
errorspgi-4-3_S11_L001_R2_001.good.fq1,378,613309,468,800100.0%224.5 bases239 bases84.8%
total2,757,226616,732,756100.0%223.7 bases239 bases91.8%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,653127.17.4100.0%Escherichia coli str. K-12 sbstr. MG1655, complete genome. Live strain from Systems Biology Research Group
total4,641,653100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000005248
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000261
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.027

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.85315

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoffOFF
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.025
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.2.8
R3.3.1

Execution Times

stepstartendelapsed
Read and reference sequence file input18:44:57 17 Mar 201818:45:41 17 Mar 201844 seconds
Read alignment to reference genome18:45:41 17 Mar 201818:50:22 17 Mar 20184 minutes 41 seconds
Preprocessing alignments for candidate junction identification18:50:22 17 Mar 201818:51:06 17 Mar 201844 seconds
Preliminary analysis of coverage distribution18:51:06 17 Mar 201818:52:51 17 Mar 20181 minute 45 seconds
Identifying junction candidates18:52:51 17 Mar 201818:52:56 17 Mar 20185 seconds
Re-alignment to junction candidates18:52:56 17 Mar 201818:53:39 17 Mar 201843 seconds
Resolving best read alignments18:53:39 17 Mar 201818:54:56 17 Mar 20181 minute 17 seconds
Creating BAM files18:54:56 17 Mar 201818:56:47 17 Mar 20181 minute 51 seconds
Tabulating error counts18:56:47 17 Mar 201818:57:38 17 Mar 201851 seconds
Re-calibrating base error rates18:57:38 17 Mar 201818:57:39 17 Mar 20181 second
Examining read alignment evidence18:57:39 17 Mar 201820:19:13 17 Mar 20181 hour 21 minutes 34 seconds
Polymorphism statistics20:19:13 17 Mar 201820:19:14 17 Mar 20181 second
Output20:19:14 17 Mar 201820:19:59 17 Mar 201845 seconds
Total 1 hour 35 minutes 2 seconds