Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,213,568 Δ48 bp coding (524‑571/1842 nt) rpoD → RNA polymerase, sigma 70 (sigma D) factor

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3213568 3213615 48 51 [0] [1] 52 rpoD RNA polymerase, sigma 70 (sigma D) factor

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 32135670 (0.000)47 (0.730) 41/264 0.5 100% coding (523/1842 nt) rpoD RNA polymerase, sigma 70 (sigma D) factor
?NC_000913 3213616 = 0 (0.000)coding (572/1842 nt) rpoD RNA polymerase, sigma 70 (sigma D) factor

AACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTG  >  NC_000913/3213426‑3213708
                                                                                                                                             |                                                                                                                                             
aaCCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCCGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAAGGCAGAAGAAGAGCTGGa                                                                                                                                              >  2:556915‑M1/1‑142 (MQ=255)
aaCCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGa                                                                                                                                              >  1:896674‑M1/1‑142 (MQ=255)
 aCCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGac                                                                                                                                             >  2:459399‑M1/1‑141 (MQ=255)
    aGGTTCAGTGCGCCGTTGATGGATGACCGGAAGCGATCACCTAGCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgat                                                                                                                                          <  1:493687‑M1/143‑6 (MQ=255)
     ggTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACTCGAACGCAGAAGGAGTTCTGGtcgatg                                                                                                                                         >  1:640548‑M1/1‑137 (MQ=255)
      gTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGCAGCGCGTCGGTCCGATCTGATCACCGGCTTGGTCGACCCGAACGCAGCAGAAGAACGGGaccatga                                                                                                                                        >  2:995723‑M1/1‑136 (MQ=255)
      gTTCAATGCTCCGGTGCTGAATATCCGGAAGCGAGCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatga                                                                                                                                        <  1:1087137‑M1/143‑8 (MQ=255)
            tGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaaga                                                                                                                                  >  2:314446‑M1/1‑130 (MQ=255)
              cTCCGTTGCTGAATATCCGGACGCGATTACCTATCTGCCGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatg                                                                                                                                <  1:320789‑M1/143‑16 (MQ=255)
                 cGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCGGCACCATCTTATCTCCGTTTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaag                                                                                                                             <  1:555112‑M1/143‑19 (MQ=255)
                  gctgcTGAATATCCGGGAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaaga                                                                                                                            <  2:58575‑M1/141‑20 (MQ=255)
                      cTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGACGAAGATCTGGacgatgacgaagatgaagacgaa                                                                                                                        >  2:715382‑M1/1‑120 (MQ=255)
                         aaTATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaa                                                                                                                     >  1:801894‑M1/1‑117 (MQ=255)
                          atatCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGCCTGTCCGATCTGATCACCGGCTACGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaaaatgaaaacgaagaag                                                                                                                    >  2:911483‑M1/1‑116 (MQ=255)
                           tatCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCATTGTTGGCCCGAACGCCGAAGAAGATCTCGacgatcacgaagaagaagaccacgaaga                                                                                                                   >  2:815376‑M1/1‑115 (MQ=255)
                              ccGGAAGCGATCACCTATCTGCGGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGAGCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatgg                                                                                                                <  2:942787‑M1/143‑32 (MQ=255)
                                ggAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcg                                                                                                              >  2:102486‑M1/1‑110 (MQ=255)
                                     cGATCACCTATCTGCTGGAACAGGACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgac                                                                                                         <  1:631142‑M1/143‑39 (MQ=255)
                                            cTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcg                                                                                                  >  1:849265‑M1/1‑98 (MQ=255)
                                               tCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaaggtggcgaggacgacagcgccg                                                                                               >  2:5786‑M1/1‑95 (MQ=255)
                                               tCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGaacgtgtcttagatgaagacgaagaagatggcgatgacgacagcgccg                                                                                               >  1:868433‑M1/1‑95 (MQ=255)
                                                gtgctggAGCAGTACGATCGTGTTGATGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgtcgaagatgaagacgaagaagatggcgatgacgacagcgccga                                                                                              <  2:988201‑M1/142‑50 (MQ=255)
                                                    tgGACCCGTAGGGTCGTGTTGCAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgat                                                                                          <  2:318385‑M1/143‑54 (MQ=255)
                                                    tgGAACAGTACGATCGGGTTGAAGCAGAAGAAGGGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgat                                                                                          <  2:102107‑M1/143‑54 (MQ=255)
                                                     ggAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatg                                                                                         >  1:1111607‑M1/1‑89 (MQ=255)
                                                     ggAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACAGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgctg                                                                                         >  1:626054‑M1/1‑89 (MQ=255)
                                                      gAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatga                                                                                        <  1:941685‑M1/143‑56 (MQ=255)
                                                      gAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTGGACCCGAACGCAGAGGAAGATCTGGacgctgacgagcatggagacgaagacaatggcgaggacgacagcgcggatagtga                                                                                        >  2:92092‑M1/1‑88 (MQ=255)
                                                              cGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgcagaagatggcgctggcgacagcgccgatgatgacaacagca                                                                                >  1:1151408‑M1/1‑80 (MQ=255)
                                                              cGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACAGGCTTTGTTGACCCGAACGCAGAAGACGACCTGGacgaagacgacgatgacgacgaaccagatggcgacgacgaccgcgccgacgacgacaacagca                                                                                >  2:455329‑M1/1‑80 (MQ=255)
                                                               gATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcat                                                                               <  1:1174327‑M1/143‑65 (MQ=255)
                                                                 tCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcg                                                                             >  2:191596‑M1/1‑77 (MQ=255)
                                                                  cgggttgaAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcga                                                                            <  1:370692‑M1/140‑68 (MQ=255)
                                                                     gtTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgaccc                                                                         >  1:158037‑M1/1‑73 (MQ=255)
                                                                                agaagCGCGTCTGTCCGATGTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctc                                                              <  2:874619‑M1/143‑82 (MQ=255)
                                                                                      gcgTGTGTCCGATCGGATCACGGGCTTTGGGGACCCGAACGCGGAAGAAGATCTGGacgatgacgaagatgaagacgaagaggatggcgatgacgacagcgccgatgatgacaacagcatcgtcccggaactggctcgcgaaa                                                        <  2:148633‑M1/143‑88 (MQ=255)
                                                                                        gTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgaccgcgccgatgatgccaacagcctcgacccggaacgggctcgcgaacaa                                                      >  2:238540‑M1/1‑54 (MQ=255)
                                                                                              ccGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcg                                                <  1:459399‑M1/143‑96 (MQ=255)
                                                                                                   cTGATCACGGGCTTTGTGGATCCGAACGCAGAAGAAGAGCTGGaggatgacgaagatgaagacgaagaagagggcaatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaact                                           <  1:500614‑M1/143‑101 (MQ=255)
                                                                                                    tGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaacta                                          >  2:171353‑M1/1‑42 (MQ=255)
                                                                                                      aTCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaaggagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaagtggctcgcgaaaaatttgcggaactacg                                        >  2:672976‑M1/1‑40 (MQ=255)
                                                                                                           gggcTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaactacgcgctc                                   <  1:1033498‑M1/142‑109 (MQ=255)
                                                                                                            ggCTTTGTTGACCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgagccggaactggctcgcgaacaatttgcggaactacgcgctcc                                  >  1:408869‑M1/1‑34 (MQ=255)
                                                                                                                      aCCCGAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaactacgcgctcagtacgttgta                        >  2:497803‑M1/1‑24 (MQ=255)
                                                                                                                          taacGCAGAAGAATATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaactacgcgctcagtacgttgtaacgc                    <  1:1070243‑M1/142‑124 (MQ=255)
                                                                                                                          gAACGCAGAAGAAGATCTGGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggcccgcgaaaactttgcggaactacgcgctcagcacggtgtaacgc                    >  2:52352‑M1/1‑20 (MQ=255)
                                                                                                                            aCGCAGAAGAAGACCTGGacgaggacgaagacgaagacgaagaggatggcaatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaactacgcgctcagtacgttgtaacgcgt                  <  2:510549‑M1/143‑126 (MQ=255)
                                                                                                                                     agtatctTGgcgatgacgaagatgaagacgaagaagagggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaactacgcgctcagtacgttgtaacgcgtgacaccatc         <  2:338101‑M1/140‑135 (MQ=255)
                                                                                                                                        acctgGacgatgacgaagatgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaactacgcgctcagtacgttgtaacgcgtgacaccatcaaa      <  1:123797‑M1/141‑138 (MQ=255)
                                                                                                                                        aTCTGGacgatgacgaagacgaagacgaagaagatggcgatgacgacagcgccgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaactacgcgctcagtacgttgtaacgcgtgacaccatcaaa      <  1:53114‑M1/143‑138 (MQ=255)
                                                                                                                                            ggacgatgacgaagatgaagccgaaaaagatggcgttgacgacagcggcgatgatgacaacagcatcgacccggaactggctcgcgaaaaatttgcggaactacgcgctcagtacgttgtaacgcgtgacaccatcaaagcga  <  2:1010059‑M1/143‑142 (MQ=255)
                                                                                                                                             |                                                                                                                                             
AACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTG  >  NC_000913/3213426‑3213708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.