New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2647583 =	40 (0.790)	4 (0.080)	4/270	6.9	9.3%	coding (4703/4962 nt)	yfhM	bacterial alpha2‑macroglobulin colonization factor ECAM, anti‑host protease defense factor, periplasmic inner membrane‑anchored lipoprotein
	?	NC_000913	2647603 =	40 (0.820)					coding (4683/4962 nt)	yfhM	bacterial alpha2‑macroglobulin colonization factor ECAM, anti‑host protease defense factor, periplasmic inner membrane‑anchored lipoprotein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAG  >  NC_000913/2647443‑2647725                                                                                                                                             |                                                                                                                                               gATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTg                                                                                                                                              >  2:501779/1‑143 (MQ=255)  aTACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTgg                                                                                                                                             <  2:163834/143‑1 (MQ=255)        cAGCGTGACCGGTTGGTATTCATCAACGGCAACCGCGGCCACGACGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTAc                                                                                                                                       <  1:887577/143‑1 (MQ=255)                       gTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCt                                                                                                                        >  2:307153/1‑143 (MQ=255)                        tATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTCCGCCAGACTcag                                                                                                                       >  1:746292/1‑141 (MQ=255)                        tATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTATGCTCCAGGCTGGCGCTACCGTGCGCCAGagtact                                                                                                                       >  2:113385/1‑138 (MQ=255)                         aTTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCggg                                                                                                                      >  2:544006/1‑143 (MQ=255)                          gtcatccACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGt                                                                                                                     <  1:612390/142‑1 (MQ=255)                           tcatgaACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCGCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGtt                                                                                                                    <  2:175388/143‑1 (MQ=255)                           tcatcaACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGtt                                                                                                                    >  2:152645/1‑143 (MQ=255)                              gcaACGGCAACCGCCGCGCGCAAGCGGTCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGTGCTACCGTTCGCCAGATTCTGGTTttc                                                                                                                 <  1:71380/142‑1 (MQ=255)                                aaCGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTACTGCACTTCGCCACCACTTTGCTCCAGGCCGGCGCTACCGTTCGCCAGATTCTGGTTttcca                                                                                                               >  2:449533/1‑143 (MQ=255)                                     catccgcggCCACAAAGCGATCGTCACGGACCTGAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTttccagttcc                                                                                                          <  1:163079/140‑1 (MQ=255)                                     cAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTttccagttcc                                                                                                          <  2:621752/143‑1 (MQ=255)                                        ccgccgCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGCTCCAGa                                                                                                       <  2:85707/143‑1 (MQ=255)                                          tgtgccACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGACGCCACGTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCCGTTCCAGAcc                                                                                                     >  1:310106/4‑143 (MQ=255)                                           ccaccACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGAccc                                                                                                    <  2:545466/140‑1 (MQ=255)                                                   aaGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCgcaggcag                                                                                            <  1:307225/143‑1 (MQ=255)                                                    aGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGgcagc                                                                                           >  1:133004/1‑143 (MQ=255)                                                        aTCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGAt                                                                                       >  2:763488/1‑143 (MQ=255)                                                         tcgtcgCGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCAATGTGTATCCTGTTTGCTCCAGGCTGGCGCGATCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATc                                                                                      <  2:885426/143‑1 (MQ=255)                                                         tCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGCTCCAGACCCGCAGGCAGCAGATc                                                                                      >  1:4652/1‑143 (MQ=255)                                                              aCGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCCGGCAGCAGATCTAcca                                                                                 >  2:640139/1‑141 (MQ=255)                                                               cGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGAc                                                                                >  2:598126/1‑143 (MQ=255)                                                               cGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGAc                                                                                >  2:595701/1‑143 (MQ=255)                                                                   tcTCGATGGGCTTGATGCTCGCTTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTGTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAAc                                                                            <  1:375208/142‑1 (MQ=255)                                                                              ttAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCAc                                                                 >  2:220282/1‑143 (MQ=255)                                                                              ttAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTGCCATGTCCAGACCCGCAGGCACACGATCTTCGATTAACGcccacgacgc                                                                 >  2:832927/1‑134 (MQ=255)                                                                                aaTGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTGTGCTCCAGGCTGGCGCTCCCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGGCCAACGGATCCGGCCCga                                                               >  1:302966/1‑142 (MQ=255)                                                                                  tGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACGTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTGTTCCAGTTCCAGACTCGAAGGCAGCAGATCGACGAGCAACGccccggccacgcgg                                                             >  1:883994/1‑130 (MQ=255)                                                                                     tCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTa                                                          >  1:869495/1‑143 (MQ=255)                                                                                        ccTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGttccgg                                                       >  1:837044/1‑139 (MQ=255)                                                                                                           tAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACcaca                                    >  1:278784/1‑143 (MQ=255)                                                                                                           tAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTCGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACcaca                                    >  2:574943/1‑143 (MQ=255)                                                                                                                    cACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCAcaccagcacca                           >  2:862706/1‑143 (MQ=255)                                                                                                                               cacTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTa                <  2:371479/143‑1 (MQ=255)                                                                                                                               cacTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCCGTTACTGGCTTTTACCTGCAACCACACCCGCACCAGACCGCCGCTa                >  1:664175/1‑143 (MQ=255)                                                                                                                                  tttGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGATAAGGGCTTTTACCTGCAAACACACCAGCACCATATCGCCGCTACGt             >  2:553133/1‑143 (MQ=255)                                                                                                                                         cAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGGTACACGACGAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGAACGCCGCTCCGTAACGAGt      >  2:778583/1‑143 (MQ=255)                                                                                                                                         cAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTCCGTACCGAGt      >  1:451120/1‑143 (MQ=255)                                                                                                                                             cTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAg  <  2:67431/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                               GATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATCTGGTTCAGTAAGTTCTGCACTTCGCCACCACTTTGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAG  >  NC_000913/2647443‑2647725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.