New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2515612	NA (NA)	66 (1.050)	49/280	0.2	95.7%	intergenic (+171/+29)	insL1/yfeA	IS186 transposase/putative diguanylate cyclase
	?	NC_000913	2536308 =	3 (0.050)					coding (477/510 nt)	crr	glucose‑specific enzyme IIA component of PTS

GAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCA  >  NC_000913/2515475‑2515750                                                                                                                                          |                                                                                                                                           gAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGGCAGCGTACAAAACGCACTTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATa                                                                                                                                       <  2:488907/143‑1 (MQ=2)      tACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCTAATTcc                                                                                                                                  >  2:458365/1‑143 (MQ=18)         aGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGCATCACGCGAGGCATCGTCTTCTCTAGGCACCGTGGCGTCGACTTCTTCAGATGCTTCAACCCTTGACCTAGCCGTCCTGGGCTAATTCGCCg                                                                                                                               >  2:465806/1‑143 (MQ=2)         aGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCCTCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGATTAAAAAATTAAATAAGCGCTTATGAGATAATACCCCg                                                                                                                               >  1:867734/1‑143 (MQ=11)         aGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCg                                                                                                                               <  2:194292/143‑1 (MQ=35)           gTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGAGAGGCATCGTCTTAACGAGGCACCGAGGCATCGCATTGTTCAGATGGTTCCACCCTTCAGTTAGCGCTTATGAGATAATTCCCCGGt                                                                                                                             >  2:562019/1‑143 (MQ=11)               ccATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGTAGTTCGCGTGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGttttt                                                                                                                         <  1:367159/143‑1 (MQ=255)                 aTTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCATTCCCCGGtgttttt                                                                                                                       >  2:800741/1‑143 (MQ=18)                       tggATAGTATCTTTGACAGTCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCTTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCt                                                                                                                 <  2:568604/142‑1 (MQ=14)                       gggCCAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCt                                                                                                                 <  2:588387/143‑1 (MQ=255)                         gACAGTATCCCTGACAGTCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGt                                                                                                               <  2:898152/143‑1 (MQ=255)                         gACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCCTCGTCTTCACGAGGCCCCGACGCGTCGCATTCTTCAGATGGTTCAACCCTTACGTTCGCGCTTATGGGATAATTCCCCGGGTCTTTTCCGccact                                                                                                               >  2:118733/1‑140 (MQ=11)                              tATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCCTATGGGATAATTGCCCGGATTTTTTACGCCAGTTCAta                                                                                                          >  2:165299/1‑142 (MQ=14)                                tCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAg                                                                                                        <  1:187328/143‑1 (MQ=255)                                   cTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGCCTAAACAACGCACCGAGGCGTCGCATCCTGCATATCGTTCAACCCTGAAGTTAGCGCTTATGCGATAATCCCCCGGTCTTCCTACGCCTGTTCACCCGCTa                                                                                                     >  2:92661/1‑143 (MQ=14)                                        aGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACGCTTAAGGTAGCGCTTGTGGGATAATTCCCCGGTTTTTTTCCGGCGGTAAATGACCTAATGGc                                                                                                >  2:779708/1‑143 (MQ=14)                                        aGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCGTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTGATCAGCTAAtagg                                                                                                >  2:651476/1‑140 (MQ=255)                                           cTACAAAACGCGATTGAAGATCGCGATGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGc                                                                                             <  1:882693/143‑1 (MQ=255)                                            tACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGACGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCAGTCAATCAGCTAATGGCTGCg                                                                                            >  1:838596/1‑143 (MQ=255)                                             aCAAACCGCACTTGCAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCgg                                                                                           <  1:71395/143‑1 (MQ=255)                                               aaaaCGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATGATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGc                                                                                         >  1:1075663/1‑143 (MQ=255)                                               aaaaCGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGc                                                                                         >  1:1095137/1‑143 (MQ=255)                                                     cAATTGAAGAACTCGTGGGATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCaa                                                                                   <  1:871198/143‑1 (MQ=255)                                                     cAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTATTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCaa                                                                                   >  1:11355/1‑143 (MQ=255)                                                       aTTGAAGAACGCGAGGCAACGTCTGAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATc                                                                                 <  1:4821/143‑1 (MQ=255)                                                            agacCGCGAGGCATCGTCTTAACGACGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATa                                                                            <  1:365094/143‑1 (MQ=255)                                                            agaaCGGGACGCACCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATa                                                                            <  1:935869/143‑1 (MQ=255)                                                             gaaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATAc                                                                           >  1:483564/1‑143 (MQ=255)                                                               aCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATAccc                                                                         <  2:333250/143‑1 (MQ=255)                                                                 gcgAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCACTCATATCCCCtt                                                                       >  1:135633/1‑143 (MQ=255)                                                                  cggggCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAGGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTCATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTg                                                                      <  2:544434/140‑1 (MQ=255)                                                                   gAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTCATCCGCTAATGGCTTCTGGCGACCAATCAAATACACttac                                                                     >  1:380618/1‑141 (MQ=21)                                                                           gTCTTAACGAGACACCGAGGCGTCGCATTCTTCAGATGGATCAACCCTTAAGTTAGCGCTTATGGGATAATTCACCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCACTCAAATACCCTTGCAGatatct                                                             >  2:191032/1‑141 (MQ=255)                                                                             cTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACTCCTGTTAATCAGCTAATGGCTGCGGGTGACCAATCACATTCTCTTGCAGATATTGCa                                                           >  2:1022852/1‑143 (MQ=25)                                                                                 aCGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCCGCGGGCGACCAACCTAATACCCTCGCAGCTATTGCGccac                                                       >  2:213714/1‑141 (MQ=25)                                                                                    aGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAg                                                    <  2:444322/143‑1 (MQ=255)                                                                                     ggCACCGAGGCGTCGCATTCGTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGc                                                   <  2:567245/143‑1 (MQ=255)                                                                                         ccGAGGCGTCGCATTCTTCAGATGGTGCAACCCTTAAGTTAGCGATTATGAGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTAt                                               <  1:1055618/143‑1 (MQ=255)                                                                                          cGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCGGGGCTTATg                                              >  2:920244/1‑143 (MQ=255)                                                                                          cGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATg                                              <  2:534707/143‑1 (MQ=255)                                                                                             gccgcgggagttctcAGATGGTTCGACCCTGAAGTGAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCaa                                           <  2:244711/130‑1 (MQ=255)                                                                                               cGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTCTGCAATa                                         >  1:886036/1‑143 (MQ=255)                                                                                                gtctgaTTCTTCAGATGGTTCATCCCTTAACTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAg                                        <  2:869611/138‑1 (MQ=255)                                                                                                 tCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATATCCTTCCAGATATTGCACCAAGAGCTTACGCAATAgc                                       >  2:285214/1‑143 (MQ=38)                                                                                                  cGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATCGGATAATTCCCCGGTTTTTTTACTCCTGTTTATCAGCTAATGCCTGCGGGCGTCCAATCAAATACCCCTCCACCCATTGCCCCCCGCGCTTATGCaacaccc                                      >  2:314715/1‑138 (MQ=25)                                                                                                     aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAGTACCGTTGCAGATATTGCACCCGGAGCTTATGCAATGGCGCct                                   >  1:881029/1‑143 (MQ=255)                                                                                                         ttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCctgctg                               >  1:921186/1‑143 (MQ=255)                                                                                                             ggtGGTTCAAGCCTTAAGTTAGCGCTTATGGGATAATTCCGCGGTTTTTGTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGt                           <  2:1082586/141‑1 (MQ=255)                                                                                                              aTGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTCTGCCATAGCGCCCGCTGCTGtt                          >  1:564786/1‑143 (MQ=255)                                                                                                                ggTTCAACCGTTAAGGTAGCGCTTATGGGATAATTCCCCGGTTTTCTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGc                        <  1:69795/143‑1 (MQ=255)                                                                                                                ggTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGc                        <  2:521767/143‑1 (MQ=255)                                                                                                                 gTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCg                       >  1:997139/1‑143 (MQ=255)                                                                                                                  ttcagtccgtAAGTGAGCGCTTATGGGATAAGTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGAGATGGCGCCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGt                      <  2:445964/134‑1 (MQ=255)                                                                                                                    cAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCGCCCTGCTCTTATGCAATAGCGCCTGCTGCTGTTGCGtct                    <  2:796392/143‑1 (MQ=255)                                                                                                                     ctcccTTCAATGAGCACTTCTGGCATAATTCCCCCGTTTTTTTACGCATGTTAATCAGCTAATGGCTGCGGCCGACCAATCAAATTCCCTTGCAGATATTGCACCCCGATCTTATTCAATAGCCCCTGCTGCTGTTGCGtctc                   <  2:827062/141‑1 (MQ=255)                                                                                                                      aCCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCg                  >  1:81107/1‑143 (MQ=255)                                                                                                                      aCCCTGAAGTCAGCGCTTATGCGCTAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCACATACCCTTGCAGTTATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCg                  <  1:125044/143‑1 (MQ=255)                                                                                                                      aCCCGTAAGTTAGCGCTGATGGGATAATTCCCCGGTTGTCTTACGCCTGTTACTCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCg                  <  1:125528/143‑1 (MQ=255)                                                                                                                         cTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCGTATGCAATAGCGCCTGCTGCTGTTGCGTATCGACa               >  1:1001/1‑143 (MQ=255)                                                                                                                                      ttATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACca  <  2:483564/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                           GAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCA  >  NC_000913/2515475‑2515750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.