Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,001,644	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001643	1 (0.020)	55 (1.000)	46/284	0.1	98.2%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001649 =	1 (0.020)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

AGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001502‑4001643 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  >  NC_000913/4001649‑4001780                                                                                                                                                                                                                                                                                    AGTGTGGATTGAGCGTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATC                                                                                                                                     <  2:620684/143‑1  GTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCT                                                                                                                                    >  1:772645/1‑143    ATGGAGTGGTCTTGGCGAACGGGACGACGACGAGCGACTGCGCGTACAATCTGAACTGGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCC                                                                                                                                  <  2:447447/143‑1      ggtcTGATCGTGTCGCATCGGACGGCGACGCGCGACTGCGCGTACAATCGGAACTTGGCCGGAGCCTGGCAACCCGCCCGGCACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTT                                                                                                                                <  1:129849/139‑1            ATCTTGTCGAACCGGACGACGACGAGCGGCTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTT                                                                                                                          >  2:408648/1‑143               TTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGCAGCATCGGCACGTTTCTTTGAAGACGACGCCGGCCTGCATCCCCTTCTTCTTCTT                                                                                                                       >  2:792063/1‑143                            CGACGACGGGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAA                                                                                                          <  1:400402/143‑1                                GACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTCTGAAGACGACGACGGCCTGCATCTCCTTCTTCTCCTTTGAAGATGCGGAAGAcc                                                                                                      >  1:153976/1‑141                                     GCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCC                                                                                                 <  1:174188/143‑1                                        ACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGTATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGT                                                                                              <  2:72341/143‑1                                         CTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCAGCACGGAACTGGAAGACATCGAAACATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGCTGCGGAAGATCTCTCCGGTc                                                                                             >  2:205523/1‑142                                          ttCGCGTACAATCTGAACTCCGCCAGCGCCTGGCAACCCGCCCGGAACTGGAAGCCCTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAA                                                                                            <  2:108681/141‑1                                            CGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTCACT                                                                                          >  1:146585/1‑143                                                    ATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTG                                                                                  <  1:743883/143‑1                                                    ATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTG                                                                                  <  1:937897/143‑1                                                       TGAAGTGGGCCAGAGCCTGGCAACCCGCCCGGCACTGGAGGTCGTGGTCGCAGCGGCACGTTTCTTTGAACTCGACGACGGCCTGCATCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCA                                                                               <  2:904600/143‑1                                                          ACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGAGACCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTACCTCCACTGTGGCATTT                                                                            <  1:936746/143‑1                                                          ACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGCCGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTT                                                                            <  2:47634/143‑1                                                           CTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTA                                                                           <  2:729728/143‑1                                                                CCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATC                                                                      >  2:863203/1‑143                                                                     GCCTGGCAGCTCGCCCGGCACTGGAAGACATCGAAGCATCGTCACGTTTCTTTGAAGACGACGACGGCCTGCATGTCCTGCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACCCCACTGTGGCATTTACCATCCGTGA                                                                 <  1:242026/143‑1                                                                      CCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCAGCGGCACGGTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT                                                                <  1:401963/143‑1                                                                       CTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCCTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTATGGCATTTACCATCCGTGAcg                                                               >  2:760183/1‑141                                                                         GGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGT                                                             >  1:791833/1‑143                                                                          GCAACCCTCCCGGAACTGGAAGACATCGAAGCGTCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTC                                                            <  1:301731/143‑1                                                                               CCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATTACAATGTGATCTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTG                                                       >  2:944199/1‑143                                                                               CCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTG                                                       >  1:652391/1‑143                                                                               CCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTG                                                       <  2:208378/143‑1                                                                                CGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGT                                                      <  1:841058/143‑1                                                                                 GCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTT                                                     <  1:449305/143‑1                                                                                  CCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTc                                                    >  1:558803/1‑142                                                                                     GGAACGTGAAGACATCGAAGCATCGGCGCGTTTCTTTGAAGACGGCGACGGGCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCCCGCCGGTAACTCCACTGTGGCATTTACCATCCGTGGTGGTCGTCTTTTTACT                                                 <  1:388988/143‑1                                                                                        ACTGGAAGACATCGAAGCATCGGCACGATTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTG                                              >  1:527295/1‑143                                                                                         CTGGAAGACAGCGAAAAACCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGC                                             <  2:253003/143‑1                                                                                            GAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTG                                          >  2:453153/1‑143                                                                                              AGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAG                                        >  2:356596/1‑143                                                                                               GACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGC                                       <  2:203611/143‑1                                                                                                  ATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGTCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTG                                    <  2:839628/143‑1                                                                                                   TCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTCACTCCACTGTGGCATTTACCATCCGTGATGGTCGCCTGTTTACTCTGCGTGAGCGTGA                                   >  1:696004/1‑143                                                                                                    CGACGCACCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTTTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                  <  2:229819/143‑1                                                                                                    CGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                  <  2:746941/143‑1                                                                                                    CGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                  <  2:772645/143‑1                                                                                                     GAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAAC                                 <  2:676378/143‑1                                                                                                              GCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGGCGTCTGTTTACTCTGCGTGAGCGTGAACTACCCGCTT                        >  1:738332/1‑143                                                                                                                 cgttacgaTGAAGACGACGACGGCCTGCATCTCCTTCGTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCCCTGTGGCATTTACTATCCGTGATGGTCGTCTGGTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTC                     <  2:136292/135‑1                                                                                                                  GTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACGGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGGGCGTGGACTGCCCGCTTTTCG                    >  2:938486/1‑143                                                                                                                  GTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCCCTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCG                    >  2:803242/1‑143                                                                                                                    TTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTC                  >  2:47221/1‑143                                                                                                                    TTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTC                  >  2:49394/1‑143                                                                                                                     TCTTTGAAGGCGACGACGGCCTGCATCTCCTTCTGCTTCTTTGATGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT                 <  1:234260/143‑1                                                                                                                          GAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATC            >  2:786253/1‑143                                                                                                                                GACGACTGCTTGCATCGCCTTCTTCTTCGGTGGGGATGCGGAAGATCAGGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCGGCGTGAGCGTGAACTGTCCGCTTTTCGTCTGTATCGTATGC      <  1:377453/143‑1                                                                                                                                GACGACGGCCTGCGACTCCTGCTTCTCCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGC      <  2:146585/143‑1                                                                                                                                GACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACTATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGC      <  2:746965/143‑1                                                                                                                                    ACGGCCCGCAGCTCCCTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGCCATTCAACCTCCGTTAGGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  <  2:854908/143‑1                                                                                                                                                                                                                                                                                    AGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001502‑4001643 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  >  NC_000913/4001649‑4001780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.