New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 182680749 (1.060)4 (0.090) 4/272 6.1 7.9% coding (117/969 nt) astE succinylglutamate desuccinylase
?NC_000913 = 1826851 46 (1.040)coding (73/969 nt) astE succinylglutamate desuccinylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1826661‑1826807
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcgttCGCTGGCGCTGGCTGGGCGATGGTGTGCTGGAACTGACGCCATTAACGCCACCGCAAGGCGCACTGGTGATTTCAGCGGGAATACACGGTAATGAGACGGCACCTGTGGAGATGCTGGACGCGTTGCTTGGCGC  <  NC_000913/1826851‑1826718
                                                                                                                                                                                                                                                                                         
GGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGG                                                                                                                                            >  1:122746/1‑143
GGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGG                                                                                                                                            <  1:328557/143‑1
      CCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCCGCATCTCCACAGGTGCCGTCTCCTTACCGTGTATCCCCCCTGCAATCACCAGTGCGCCTTGCGGTGGCGTTCc                                                                                                                                      >  1:574799/1‑142
      CCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGgtaa                                                                                                                                      >  1:596162/1‑139
                                    GGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTCGCTGGCGCTGGCTGGGCGATGGTGTGCTGGA                                                                                                        >  2:103468/1‑143
                                                                       GTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTTCGCTGGCGCTGGCTGGGCGATGGTGTGCTGGAACTGACGCCATTAACGCCACCGCAAGGCGCACTGG                                                                     <  1:103468/143‑1
                                                                                                                                          aaTGGCGCTCGCTGGCGCTGGCTGGGCGATGGTGTGCTGGAACTGACGCCATTAACGCCACCGCAAGGCGCACTGGTGATTTCAGCGGGAATACACGGTAATGAGACGGCACCTGTGGAGATGCTGGACGCGTTGCTTGGCGC  <  1:329072/141‑1
                                                                                                                                                                                                                                                                                         
GGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTTGCGGTGGCGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1826661‑1826807
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcgttCGCTGGCGCTGGCTGGGCGATGGTGTGCTGGAACTGACGCCATTAACGCCACCGCAAGGCGCACTGGTGATTTCAGCGGGAATACACGGTAATGAGACGGCACCTGTGGAGATGCTGGACGCGTTGCTTGGCGC  <  NC_000913/1826851‑1826718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.