New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1826807	49 (1.060)	4 (0.090)	4/272	6.1	7.9%	coding (117/969 nt)	astE	succinylglutamate desuccinylase
	?	NC_000913	= 1826851	46 (1.040)					coding (73/969 nt)	astE	succinylglutamate desuccinylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGA  >  NC_000913/1826667‑1826949                                                                                                                                              |                                                                                                                                               ccGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCCGCATCTCCACAGGTGCCGTCTCCTTACCGTGTATCCCCCCTGCAATCACCAGTGCGCCTT‑GCGGTGGCGttcc                                                                                                                                              >  1:574799/1‑141 (MQ=255) ccGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTaa                                                                                                                                              <  1:681871/143‑1 (MQ=255) ccGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGGTaa                                                                                                                                              >  1:596162/1‑143 (MQ=255)             cgCAACCGCCAGCGTACCGGCACAGCGCCGTGATAGATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTc                                                                                                                                  <  2:521455/142‑1 (MQ=255)             agcgtccGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTc                                                                                                                                  <  2:451592/138‑1 (MQ=255)               cgcccGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCa                                                                                                                                <  2:453515/140‑1 (MQ=255)               cgaCCGGCAACGCAACGGGACCTCGCCTTGAGATATCGCGCCAAGCAACGCGTCCAGCATCGCCACAGGGGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCa                                                                                                                                <  1:537198/141‑1 (MQ=255)               caccggcaagcGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCACTACCGTGTATTCCAGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCa                                                                                                                                <  2:375055/133‑1 (MQ=255)                 aCCGCCAACGGAACGGGATCGCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGGGCCGTCGCATTGCCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGc                                                                                                                              <  1:150299/143‑1 (MQ=255)                    gccagcggcccGGGGTCCCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCAGTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGcaca                                                                                                                           <  2:631084/133‑1 (MQ=255)                      cAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCAAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCATT‑GAGGTTGCGGTGGTTGCGTCAGTTCCAGCACAcc                                                                                                                         >  1:756837/1‑143 (MQ=255)                      cAACATAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCCCCACAGGTGCCGACTCATAAACAAGTCTTGCAGCTGCAAAAACCAGTGCGCCTT‑CAGGACGCGTAAAAGGCGACaaaaacaggacaac                                                                                                                         >  2:280686/1‑130 (MQ=255)                        aCGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGGTCCAGCACACCAt                                                                                                                       >  1:166063/1‑143 (MQ=255)                          gttaCGGTATCTCGCCGTGACATATCGCGCCACACAACTCGTTCATCCTCCTCACATGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCg                                                                                                                     <  2:739649/140‑1 (MQ=255)                                 gATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCccagcc                                                                                                              >  2:332667/1‑143 (MQ=255)                                     tcGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCACTGAAATCACCAGTGCGCCTT‑GCGGTGGCCTTAATCGCGTCAGTCCCAGCACACCATCGccagcccaccc                                                                                                          >  2:523084/1‑134 (MQ=255)                                       gCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTGCCAGCACACCATCGCCCAgacagcgcc                                                                                                        >  2:586632/1‑143 (MQ=255)                                        ccGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCACTGAAATCACCAGTGCGCCAT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCGTCACCCAgccagcgcca                                                                                                       >  2:409676/1‑143 (MQ=255)                                        ccGCGAGAGATCGCGCCAAGCAGCGCGGCAAGCACCACCACAGGTGCCGCCTCATTACCGTGTATTCCCGCTGACATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAgccagcgcca                                                                                                       <  1:332514/143‑1 (MQ=255)                                         cgccagctatCGCGCCAAGCAACGCCTCCAGCACCTCCACAGGTGCCGTCTCATTACCGTGCATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAgccagcgccag                                                                                                      <  1:351356/136‑1 (MQ=255)                                         cGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAgccagcgccag                                                                                                      >  1:591386/1‑143 (MQ=255)                                                    gcgcCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAGCGCCAtt                                                                                           >  1:329072/1‑143 (MQ=255)                                                     cgcCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCCTCGCCCAGCCAGCGCCAGCGAACGCCGTTg                                                                                          >  1:464321/1‑143 (MQ=255)                                                       ccAAGCAGCGCGTCCAGCAGCTCCAGGTGTGCCGTCTCATTAGGGTGTAGTGGCTC‑GCAAGCAGCCGCCTGGTTTGGCGGTGGAGTTAATGGCCTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGAt                                                                                        <  2:783277/143‑1 (MQ=255)                                                        cAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGCTGAtt                                                                                       >  2:451575/1‑143 (MQ=255)                                                        cAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAATGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCCCACCATCGCCCAGCCAGCGCCAGCGAACGCCGCTGAtc                                                                                       >  2:206705/1‑142 (MQ=255)                                                         aaGCAACGCGTCCAGCATCTCCACAGGTCCCGTCTCATTACCGTGTATTCCCGCTGAAATCACGAGTGCCCCTT‑GCGCTGGCGCCCATGGCGTCAGGTCCAGCACACCGTCGCCCCCCCGGCGCCCGCGACCCCCGCTgctag                                                                                      >  2:719028/1‑139 (MQ=255)                                                            cAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCCGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGGGCCAGCGGACGCCGTTGACTtctc                                                                                   >  2:801708/1‑143 (MQ=255)                                                                 cgTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTctcgctcg                                                                              <  2:540989/143‑1 (MQ=255)                                                                     cAGCACCTCGACAGGTGCCGTCTCATTACCGTGTATGCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGa                                                                          <  2:367504/143‑1 (MQ=255)                                                                                  ggTGCCGTCCCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTAAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGtttttt                                                             <  1:421511/143‑1 (MQ=255)                                                                                   gTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCGGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGttttttg                                                            >  1:709713/1‑142 (MQ=255)                                                                                   gTGCCGGCTCATTACCGGGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTa                                                            <  1:326066/143‑1 (MQ=255)                                                                                      ccGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGGTCCAGCACACCATCGCCCAGCCAGCGCCAGGGAACGCCGTGGAGTGCACACTCGCGGATAACCGGTCTTGCAccc                                                         >  2:234991/1‑143 (MQ=255)                                                                                            cATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAg                                                   <  1:330610/143‑1 (MQ=255)                                                                                             aTTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCCGCCAGCGCCAGCGAACGCCGCTGATTTCTCGCTCGGTGATAACCGGTTTTTTCCCCGTTACgg                                                  >  2:374761/1‑143 (MQ=255)                                                                                                           ccGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCATCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGaaaa                                    >  1:506303/1‑143 (MQ=255)                                                                                                                   aTCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTGTACCCGTTCCGGTCAGAGCCAGAAACTTATCCAt                            >  2:554115/1‑143 (MQ=255)                                                                                                                     caccacTGCGCCTT‑GCGGTGGCGGTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTg                          <  1:487903/143‑1 (MQ=255)                                                                                                                      aCCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACGCGTGAAGGTCAGAGGAGGGAAAGTATCCATGGc                         >  1:14267/1‑143 (MQ=255)                                                                                                                        cAGTGCGCCTG‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGccc                       <  2:636830/143‑1 (MQ=255)                                                                                                                         aGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGcccc                      >  2:13892/1‑143 (MQ=255)                                                                                                                              gcCTC‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCccctccc                 <  2:333479/143‑1 (MQ=255)                                                                                                                                  t‑GCGGTGGCGTTAATGGCGGCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTcgc             <  1:308641/143‑1 (MQ=255)                                                                                                                                        tGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGGACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTACGGTCAGAGCACGAAAATCATCGATTGCCCCCTCCCTCGCGCCgg        >  2:175594/1‑143 (MQ=255)                                                                                                                                           cggtAATGGCGGCAGTTCCAGCACACCATCGCGCAGCTATTTGTTTCGTACGCCGTTGATTGCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAAc     <  2:166063/140‑1 (MQ=255)                                                                                                                                           cggtAATGGCGGCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTGCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAAc     <  1:147949/140‑1 (MQ=255)                                                                                                                                            gTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACg    >  1:79878/1‑143 (MQ=255)                                                                                                                                            gTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGAGCCAGCGAACGCCGTTGCTTTCTCGCCCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGACAACGAAAAGTAGCCATCGCCGCCTCCCTCCAGCTgtgacc    >  2:172959/1‑138 (MQ=255)                                                                                                                                             ttAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACgg   >  1:478046/1‑143 (MQ=255)                                                                                                                                              tAATGGCGTCAGTTCCAGCACACCATCGCGCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGa  <  2:452257/143‑1 (MQ=255)                                                                                                                                              |                                                                                                                                               CCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCTGAAATCACCAGTGCGCCTT‑GCGGTGGCGTTAATGGCGTCAGTTCCAGCACACCATCGCCCAGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTTTTTTACCCGTTAAGGTCAGAGCAAGAAAATTATCCATTGCCCCCTCCCTCGCGCTGGAACGGA  >  NC_000913/1826667‑1826949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.