Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,992,523	(CGGGC)2→1	coding (1372‑1376/2547 nt)	cyaA →	adenylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3992521 =	1 (0.020)	38 (0.890)	37/270	0.1	97.5%	coding (1370/2547 nt)	cyaA	adenylate cyclase
	?	NC_000913	= 3992515	1 (0.020)					coding (1364/2547 nt)	cyaA	adenylate cyclase

TATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGC  >  NC_000913/3992393‑3992663                                                                                                                                 |                                                                                                                                               caggagatcgccgtgctgacgcgtaagcggtacgccgcgtctgaagcattcccaggtaaagtgacgctggtaaccccgcagatttcacccgatctctcggaaccgcatctgacctttatttatgtgccGCCGGGCTAACCGtt                                                                                                                                  >  2:78777‑M1/129‑143 (MQ=255)  aggatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAaccgctc                                                                                                                                 <  2:363094‑M1/16‑4 (MQ=255)     ttatcggcgtgctgccgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAgg                                                                                                                              >  2:542800‑M1/125‑143 (MQ=255)      gatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGt                                                                                                                             >  1:707732‑M1/124‑143 (MQ=255)            cgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTAt                                                                                                                       >  1:398917‑M1/118‑143 (MQ=255)                 tgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgacatctcggctcccactctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGta                                                                                                                  <  1:178213‑M1/31‑1 (MQ=255)                    cgcgtgagctgtctgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATaa                                                                                                               >  2:673538‑M1/110‑143 (MQ=255)                       gtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTCTAACcg                                                                                                            <  1:460821‑M1/37‑1 (MQ=255)                         aagcggtatgccgcgtttgaagcattaccaggtaaagcgccgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcgcg                                                                                                          >  2:109839‑M1/105‑143 (MQ=255)                           gctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTCTCTGTATCACcgcgcg                                                                                                        <  2:155577‑M1/41‑1 (MQ=255)                            ctgtacgcggcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCgcgcgc                                                                                                       >  1:483984‑M1/102‑143 (MQ=255)                            ccgcacgccgcgtgtgaagcattaccagctaacgcgacgctggtaaacccgcacatttcacccgctctctcggtaccgcatctgtcctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCgcgcgc                                                                                                       >  1:93569‑M1/102‑143 (MQ=255)                                 tgccgggtttgaagcgttaccaggtacagtgatgcggccaatcccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAat                                                                                                  >  1:739861‑M1/97‑143 (MQ=255)                                    cgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAAGAtt                                                                                               <  1:467814‑M1/50‑1 (MQ=255)                                      cgtttgaagcattaccgggtaaagtgacgctgttaaacccgcagatttctcccgagctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGa                                                                                             >  2:418713‑M1/92‑143 (MQ=255)                                              gcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGCGCTAACCGCACAGGTTAGTACTTTGATTACCGAGCGCCAAATACTGAGtcgaaca                                                                                     <  2:343963‑M1/60‑4 (MQ=255)                                                       ggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgtcttttatttatgtgccGCTGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatc                                                                            >  1:306211‑M1/75‑143 (MQ=255)                                                           aagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCatcagccatcagcc                                                                        <  2:77639‑M1/73‑1 (MQ=255)                                                                          acccgcagatttcccccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCg                                                         >  2:510693‑M1/56‑143 (MQ=255)                                                                                 gatttcgcccgatctctcggaaccgaatctgccctttatttatgggccGCCGGGCTAACCGTTCTGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTg                                                  >  2:633471‑M1/49‑143 (MQ=255)                                                                                 gatttcacccgatctctcggaaccgaatctgaccgttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCACCAGCCATCAGCCGCTGGAACATAACCGTTACcta                                                  <  2:90848‑M1/95‑2 (MQ=255)                                                                                       ccccgacctctcggatcggaatcggacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATACCCGCGCGTCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATaaa                                            >  2:729998‑M1/43‑143 (MQ=255)                                                                                       acccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATaaa                                            <  1:713159‑M1/101‑1 (MQ=255)                                                                                        cccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAg                                                                                            <  1:646402‑M1/53‑1 (MQ=255)                                                                                        cccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAg                                                                                            >  2:646402‑M1/42‑94 (MQ=255)                                                                                         ccgatctctcggaaccgcatctgacctttatttatgtgccGCCGGGCTCACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACt                                          >  2:173612‑M1/41‑143 (MQ=255)                                                                                          cgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACtg                                         >  1:3607‑M1/40‑143 (MQ=255)                                                                                           gatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAACTATTGAGTCCATCATCAGCCCTCAGCCGCTGGAATATCACCGGTACCCGAATCAActgc                                        <  2:487156‑M1/105‑2 (MQ=255)                                                                                              ggAACCGAATCTGACCTTTATTTATGTGCC‑‑‑‑‑GCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCCGCCGCTGCTTTATTCCCGTTTGCTCAATaaaattatggtgagg                                >  1:173692/1‑131 (MQ=255)                                                                                                 tcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGt                                  <  1:522320‑M1/111‑1 (MQ=255)                                                                                                   ggcaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCCGGTGGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTgg                                >  2:555001‑M1/31‑143 (MQ=255)                                                                                                       ccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATCTTGAGTCGATCATCAGCCATCAGCCGATGGAATATAAACGTTAACTGAATCAACTGGTGGCGCGggcca                            <  2:44065‑M1/117‑3 (MQ=255)                                                                                                             ctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTa                      >  1:131505‑M1/21‑143 (MQ=255)                                                                                                                ttctttttttgtgtgccGCCGGGCTAACCGTTCAGGTTGGTATGTGTATAACCGCGCGCCAAATAGTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAGCCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACg                   >  2:51709‑M1/18‑143 (MQ=255)                                                                                                                  ctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATAGTTTAACGGc                 <  1:393541‑M1/128‑1 (MQ=255)                                                                                                                    ttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTTTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCATCCGCTGTAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCct               >  1:363094‑M1/14‑143 (MQ=255)                                                                                                                     catttacgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCctg              >  1:304696‑M1/13‑143 (MQ=255)                                                                                                                             tgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTc      >  1:77639‑M1/5‑143 (MQ=255)                                                                                                                              gccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTcg     >  1:510090‑M1/4‑143 (MQ=255)                                                                                                                                 GCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATAATCAGCCAACAGCAGATGGAATATGACAGTTACCTGAATAAACTGGTAGAGTGGGCGAGGTTTAACCGCATGCTGAACACgcgc  <  2:388578‑M1/143‑1 (MQ=255)                                                                                                                                 |                                                                                                                                               TATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCGC  >  NC_000913/3992393‑3992663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.