Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,992,523	(CGGGC)2→1	coding (1372‑1376/2547 nt)	cyaA →	adenylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3992521 =	1 (0.020)	38 (0.890)	37/270	0.1	97.5%	coding (1370/2547 nt)	cyaA	adenylate cyclase
	?	NC_000913	= 3992515	1 (0.020)					coding (1364/2547 nt)	cyaA	adenylate cyclase

CAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGA  >  NC_000913/3992388‑3992655                                                                                                                                |                                                                                                                                             cAGGAGATCGCCGTGCTGACGCGTAAGCGGTACGCCGCGTCTGAAGCATTCCCAGGTAAAGTGACGCTGGTAACCCCGCAGATTTCACCCGATCTCTCGGAACCGCATCTGACCTTTATTTATGTGCCgccgggctaaccgtt                                                                                                                               >  2:78777‑M2/1‑128 (MQ=255)  aGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgctc                                                                                                                              <  2:363094‑M2/143‑17 (MQ=255)     ttatcGGCGTGCTGCCGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcagg                                                                                                                           >  2:542800‑M2/2‑124 (MQ=255)      gatcGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggt                                                                                                                          >  1:707732‑M2/2‑123 (MQ=255)            cGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtat                                                                                                                    >  1:398917‑M2/1‑117 (MQ=255)                 tGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGACATCTCGGCTCCCACTCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgta                                                                                                               <  1:178213‑M2/143‑32 (MQ=255)                    cgcgTGAGCTGTCTGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataa                                                                                                            >  2:673538‑M2/1‑109 (MQ=255)                       gTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtctaaccg                                                                                                         <  1:460821‑M2/143‑38 (MQ=255)                         aaGCGGTATGCCGCGTTTGAAGCATTACCAGGTAAAGCGCCGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcg                                                                                                       >  2:109839‑M2/1‑104 (MQ=255)                           gCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtctctgtatcaccgcgcg                                                                                                     <  2:155577‑M2/143‑42 (MQ=255)                            ccgcacgccgcgtGTGAAGCATTACCAGCTAACGCGACGCTGGTAAACCCGCACATTTCACCCGCTCTCTCGGTACCGCATCTGTCCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgc                                                                                                    >  1:93569‑M2/7‑101 (MQ=255)                            cTGTACGCGGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgc                                                                                                    >  1:483984‑M2/1‑101 (MQ=255)                                 tGCCGGGTTTGAAGCGTTACCAGGTACAGTGATGCGGCCAATCCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaat                                                                                               >  1:739861‑M2/1‑96 (MQ=255)                                    cgcgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaagatt                                                                                            <  1:467814‑M2/143‑51 (MQ=255)                                      cgTTTGAAGCATTACCGGGTAAAGTGACGCTGTTAAACCCGCAGATTTCTCCCGAGCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattga                                                                                          >  2:418713‑M2/1‑91 (MQ=255)                                              gCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgcgctaaccgcacaggttagtactttgattaccgagcgccaaatactgagtcgaaca                                                                                  <  2:343963‑M2/143‑61 (MQ=255)                                                       ggTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGTCTTTTATTTATGTGCCgctgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatc                                                                         >  1:306211‑M2/1‑74 (MQ=255)                                                           aaGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagcc                                                                     <  2:77639‑M2/143‑74 (MQ=255)                                                                          aCCCGCAGATTTCCCCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccg                                                      >  2:510693‑M2/1‑55 (MQ=255)                                                                                 gATTTCGCCCGATCTCTCGGAACCGAATCTGCCCTTTATTTATGGGCCgccgggctaaccgttctggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctg                                               >  2:633471‑M2/1‑48 (MQ=255)                                                                                 gATTTCACCCGATCTCTCGGAACCGAATCTGACCGTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcaccagccatcagccgctggaacataaccgttaccta                                               <  2:90848‑M2/143‑96 (MQ=255)                                                                                       ccccgACCTCTCGGATCGGAATCGGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtatacccgcgcgtcaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaa                                         >  2:729998‑M2/2‑42 (MQ=255)                                                                                       aCCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaa                                         <  1:713159‑M2/143‑102 (MQ=255)                                                                                        cccGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgag                                                                                         <  1:646402‑M2/94‑54 (MQ=255)                                                                                        cccGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgag                                                                                         >  2:646402‑M2/1‑41 (MQ=255)                                                                                         ccGATCTCTCGGAACCGCATCTGACCTTTATTTATGTGCCgccgggctcaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaact                                       >  2:173612‑M2/1‑40 (MQ=255)                                                                                          cGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactg                                      >  1:3607‑M2/1‑39 (MQ=255)                                                                                           gATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaactattgagtccatcatcagccctcagccgctggaatatcaccggtacccgaatcaactgc                                     <  2:487156‑M2/143‑106 (MQ=255)                                                                                                 tcGGAACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgt                               <  1:522320‑M2/143‑112 (MQ=255)                                                                                                   ggCACCGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttccggtgggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgg                             >  2:555001‑M2/1‑30 (MQ=255)                                                                                                   ggAACCGAATCTGACCTTTATTTATGTGCC‑‑‑‑‑GCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCCGCCGCTGCTTTATTCCCGTTTGCTCAATaaaattatggtgagg                        >  1:173692/1‑131 (MQ=255)                                                                                                       ccGAATCTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatcttgagtcgatcatcagccatcagccgatggaatataaacgttaactgaatcaactggtggcgcgggcca                         <  2:44065‑M2/143‑118 (MQ=255)                                                                                                             cTGACCTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggttta                   >  1:131505‑M2/1‑20 (MQ=255)                                                                                                                ttcttTTTTTGTGTGCCgccgggctaaccgttcaggttggtatgtgtataaccgcgcgccaaatagtgagtcgatcatcagccatcagccgctggaatatagccgttacctgaataaactggtggcgtgggcatggtttaacg                >  2:51709‑M2/3‑17 (MQ=255)                                                                                                                  cTTTATTTATGTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatagtttaacggc              <  1:393541‑M2/143‑129 (MQ=255)                                                                                                                    ttatttatGTGCCgccgggctaaccgttcaggttggtatctttataaccgcgcgccaaatattgagtcgatcatcagccatcatccgctgtaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcct            >  1:363094‑M2/1‑13 (MQ=255)                                                                                                                     catttacgTGCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctg           >  1:304696‑M2/2‑12 (MQ=255)                                                                                                                             tgCCgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctgctgacctc   >  1:77639‑M2/1‑4 (MQ=255)                                                                                                                              gccgccgggctaaccgttcaggttggtatctgtataaccgcgcgccaaatattgagtcgatcatcagccatcagccgctggaatataaccgttacctgaataaactggtggcgtgggcatggtttaacggcctgctgacctcg  >  1:510090‑M2/1‑3 (MQ=255)                                                                                                                                |                                                                                                                                             CAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGA  >  NC_000913/3992388‑3992655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.