New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3224520 =	48 (1.250)	3 (0.080)	3/276	5.5	6.1%	coding (1890/3093 nt)	ebgA	evolved beta‑D‑galactosidase, alpha subunit
	?	NC_000913	3224534 =	45 (1.210)					coding (1904/3093 nt)	ebgA	evolved beta‑D‑galactosidase, alpha subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTCACCGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGAGCGTTTCACCTTCGGCGCGCACCTCTGCGTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3224663‑3224520 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgcGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC  >  NC_000913/3224534‑3224673                                                                                                                                                                                                                                                                                              GTCACCGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGAGCGTTTCACCTTCGGCGCGCACCTCTGCGTG                                                                                                                                               <  1:550867/143‑1      CGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGCGCGTTTCACCTTCGGCGCGCACCTCTGCGTGCGggg                                                                                                                                          >  2:453768/1‑140      CGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGAGCGTTTCACCTTCGGCGCGCACCTCTGCGgggaggg                                                                                                                                          >  2:17241/1‑136                                                                                                                              GGCGCGCACCTCTGCGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAA                                                                                                        <  1:514459/57‑1                                                                                                                              GGCGCGCACCTCTGCGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAA                                                                                                        >  2:514459/1‑57                                                                                                                                              TGCGCGTCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC  <  1:597094/143‑1                                                                                                                                                                                                                                                                                              GTCACCGTAATGTTGAGGAACGCTTCGCGGGCGTCCAGCTGCGGCAGCGTGATCTGCAAGGGGGCTTCGCTGTTCGGCGCAACGTCGCGCAGTTTAATCTGCTGCGTCGCGAGCGTTTCACCTTCGGCGCGCACCTCTGCGTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3224663‑3224520 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgcGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC  >  NC_000913/3224534‑3224673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.