| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 3224520 = | 48 (1.250) | 3 (0.080) | 3/276 | 5.5 | 6.1% | coding (1890/3093 nt) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
| ? | NC_000913 | 3224534 = | 45 (1.210) | coding (1904/3093 nt) | ebgA | evolved beta‑D‑galactosidase, alpha subunit | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
GGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC > NC_000913/3224395‑3224673 | ggACCGGGCCGGAAAGGGTGGCACAAGGTTAGCGCGGCGGTAAAACTCCAGACGCGGGATCTGAGTCGCCTGGAGTTGAAAGTCGAAAATAGACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcgcgc < 1:656249/143‑1 (MQ=255) ggtccTGAAAGAGTACGAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg < 1:608860/140‑1 (MQ=255) gggCCTGAACGAGTACAAACAGTTTATCGAGCAATCCACAATCCACTCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg < 1:295351/143‑1 (MQ=255) cccgAAAGAGTACTACCAGGTTATCGCGCCGCTAAGTATCCACGCGCGTGTGCTGACTCGCGGCGAGTGCAAACTCGAAAATCCACCTTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTg < 1:79998/140‑1 (MQ=255) ccTGAAAGAGTACAAGCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTGGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTg < 1:147385/143‑1 (MQ=255) cTGAAAGAGTACAAACAGGTTAGCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGa < 2:364875/143‑1 (MQ=255) gAAAGAGTACAAACAGGTTATGGCGCCGGTAAAAAGCCACGCGCGGGATCGGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaaa < 2:347111/143‑1 (MQ=255) gAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaac > 2:621348/1‑142 (MQ=255) aaGAGTACAAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACg < 1:343776/143‑1 (MQ=255) aaCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCCGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTAGAAGACTACGCCCTGCACGCCGAGGCGCGCGCAGAAGGTGAAAGGCCGGCGACg > 1:45068/1‑143 (MQ=255) gTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGCGTTGAACGTCGAAACTAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCCACccagcg > 1:605739/1‑142 (MQ=255) tATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAACATAAACTGTGGGTTACCACGCTTGATGACTACACCCTGCAAGGAGAGGGGACCCCCCGAGGCGAAACGCTCGCGGCGCAGCAGa > 1:652501/1‑143 (MQ=255) aTCGCGTCGGTAAAAATCCACGCGCGGGCTCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGAt < 1:274955/143‑1 (MQ=255) cgcCGGTAAAAATCCACGCGCGGGTTCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTaaa < 2:170488/143‑1 (MQ=255) acgcaggcgcgGCACGTGGCTCGAGGGGGGTTGAAGGGCGAAAATAAGCTGTGGTTTACCACGCTTGATGACTACCCCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGt < 1:136206/137‑1 (MQ=255) cgcgcgGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTgcgc < 2:232549/143‑1 (MQ=255) gcgcgGGATCTAACTCGCGGCGAGATGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATACCTACACCCTACACGCAGAGGTACGCGCCGAAGGAGAAACACTCGCGACGAAGCAGAACAAACAgcgccccctacacac > 1:186433/1‑132 (MQ=255) gctCTGACTCGCGGCGAGGTGAAAGTCGAGTATACACTCGGGCGTACCGCGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAg < 1:137839/141‑1 (MQ=255) gATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAg < 2:564330/143‑1 (MQ=255) tCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGCGCAGCGAAGccc > 2:356451/1‑143 (MQ=255) tCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGccc > 2:552630/1‑143 (MQ=255) cggcgAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGCACAGCGAAccccccc > 1:28819/1‑142 (MQ=255) ttGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTACACTGCGCGACGTGGCGCCGAACAGCGAAGcccccacgccgac > 1:305306/1‑135 (MQ=255) tCGAAACTAAACTGTGGTTTACTACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTg < 1:571019/143‑1 (MQ=255) cGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGc < 2:374693/143‑1 (MQ=255) cGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGACCGCCGAAGGTGAACCGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGc > 2:155447/1‑143 (MQ=255) gtaagtAAACTGTGCTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGcc < 2:119479/138‑1 (MQ=255) tgtgGGTGACCGCTCTTGGTGACTATACCCTGCGCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGAc < 1:602125/143‑1 (MQ=255) cTTGATTACTACACCCTGCACGCATAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGtt < 2:345342/143‑1 (MQ=255) gaggaCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCt < 1:149032/140‑1 (MQ=255) gaCTACACCCTGCTCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGGTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCaa < 2:593409/143‑1 (MQ=255) gaCTACACCCTGCACGCATAGGTGCGCGCCGAAGGCGCAACGCTCGCGACGCAGCAGATGAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCTCCTTGCAGATCTCGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCaa < 1:46793/143‑1 (MQ=255) gaCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CCGCTGGACGCCCGCGACGCGTTCCTCaa > 2:575095/1‑143 (MQ=255) aCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGAACACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGCTCCTCAAc > 1:131105/1‑143 (MQ=255) cTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACa < 2:42899/143‑1 (MQ=255) acacCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCCAACAGCGAAGCCCACTTATAAAAC‑CATTAACGTCAGCTGGACGCCCGCGAAGCGTTCCTCAACAtt > 2:488098/1‑143 (MQ=255) cacCCTGCACGCAGAGGTGCGCGCCGAAGGCAAGAATCCCGCTGCGCAGCAGATTAAACTGCGCGGCGATGCGCCGAACAGCGCACCCCCCTCGCAGATCACGCTGCCC‑CAGCCGCACGCCCGCCAAGCGATCCTCAACCTTa > 1:171377/1‑143 (MQ=255) acCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAACCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGCAGCGGTCCTCAACAttgc > 2:294303/1‑141 (MQ=255) ccccGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCGCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg < 2:453768/143‑1 (MQ=255) cccTGCACGCAGAGGTGCGCGCCGAAGGTGAACCGCTCGCGACGCAGCAGATTAAACTGCGAGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg < 1:202055/143‑1 (MQ=255) cccTCCCCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg < 2:17241/143‑1 (MQ=255) ccTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAGCCGTTCCTCAACATTACgg > 2:675969/1‑143 (MQ=255) ccTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACgg < 1:494082/143‑1 (MQ=255) cACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGAc > 1:550867/1‑143 (MQ=255) gCAGAGGTGCGCGCCGAAGGTGAGACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCaa < 1:87874/143‑1 (MQ=255) cggagGTGCGCGCCGAAGGGGAACCGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCaaa < 2:92088/141‑1 (MQ=255) tGCGCGTCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTcc < 1:597094/143‑1 (MQ=255) | GGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC > NC_000913/3224395‑3224673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |