Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,001,644	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001643	2 (0.040)	36 (0.720)	33/282	0.5	94.7%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001649 =	2 (0.040)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAG  >  NC_000913/4001509‑4001786                                                                                                                                             |                                                                                                                                           tatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatctc                                                                                                                                          <  2:349914‑M2/3‑1 (MQ=255)  atggtttgatcttgtcgaaccgtacgacgacgagcgactgcgcgtacaagctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                         <  2:678685‑M2/4‑1 (MQ=255)    ggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatctccgt                                                                                                                                       >  2:221385‑M2/138‑141 (MQ=255)     gattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttc                                                                                                                                      >  2:434865‑M2/137‑143 (MQ=255)     gattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttc                                                                                                                                      <  2:812773‑M2/7‑1 (MQ=255)        tgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttc                                                                                                                                   <  1:205103‑M2/10‑1 (MQ=255)           tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttc                                                                                                                                >  2:168802‑M2/131‑143 (MQ=255)                      cggacgacgacgagcaacagcgcggagaagctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                                     <  2:782516‑M2/24‑1 (MQ=255)                             cgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgcttctttgaggacgacgacggcctgcatCGCCTTCTtcttcttcgaagccgcgcaaact                                                                                                              >  1:816982‑M2/113‑132 (MQ=255)                                 gagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACg                                                                                                          >  2:472391‑M2/109‑143 (MQ=255)                                  cgcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGc                                                                                                         <  2:51992‑M2/36‑1 (MQ=255)                                    cgactgcgcgtacaatctgaacttggccagaggctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                                       <  2:616257‑M2/38‑1 (MQ=255)                                          cgcgtacaatctgaacttggccagagcctggcaacccgcccgcaactggaagacatcgaagcatcggcacgttgctttgaagacgacgacggcccgcatCTCCGTCTTCTCCTTTGAAGCTGCGGgggatcacccccgcgccg                                                                                                 >  1:468726‑M2/100‑136 (MQ=255)                                             ggcTGAACATGGCCAGCGCCGGGCAACCGGGCCGGAACTGGAAGACAGCGGAGCAGCGGGACGTTTCTGTTAAGACGACGACGGTCTGCAT‑‑‑‑‑CTCCTTCGTCTTCTTGGAAGATGCGGAAGAGCACGCCGGTAACTCCACtgtg                                                                                         <  1:837839/141‑1 (MQ=255)                                                  atctgaacttggccagagccgggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgacgacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgtg                                                                                         <  2:423437‑M2/52‑1 (MQ=255)                                                          ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACCGCGGCATCTAc                                                                                 >  2:489349‑M2/84‑143 (MQ=255)                                                                    cctcgcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAt                                                                       <  2:484614‑M2/70‑1 (MQ=255)                                                                        gcaacccggccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑Ggtc                                                                  <  2:701340‑M2/74‑1 (MQ=255)                                                                        gcaacccgcccggaactggaagacatcgaagcatcggcacgtttccttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑Ggtc                                                                  <  2:848790‑M2/74‑1 (MQ=255)                                                                          aacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑Ggtcgt                                                                <  2:821318‑M2/76‑1 (MQ=255)                                                                           acccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCCTCCGTGCT‑Ggtcgtc                                                               >  2:36349‑M2/67‑143 (MQ=255)                                                                            cccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCt                                                              >  2:413884‑M2/66‑143 (MQ=255)                                                                              cgcccggaactggaagacatcgaagcatcggcacgcttccttgaagacgacgacggcctgcatCTCCCTCTTCTTCTTTGAAGATGCGGAAGACCACGCCGGCAACTCCACTGTGGCATTTACCATCAGTGAT‑GGTCGCCTGt                                                            >  1:203454‑M2/64‑143 (MQ=255)                                                                                       ctggaagacatcgaagcatcggcacgtttatttgaatacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTgc                                                   <  2:681732‑M2/89‑1 (MQ=255)                                                                                         ggcagacatcgaagcatcggcacgtttcttggaagacgacgacggcctgcatCTCCTTCGTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACGCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTgcgt                                                 <  1:141136‑M2/91‑1 (MQ=255)                                                                                               catcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTgcgtgagcgt                                           <  2:450894‑M2/97‑1 (MQ=255)                                                                                                  cgtggggtcggcacgttgctttgaagccgacgatggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGTTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGaa                                        <  1:297‑M2/100‑1 (MQ=255)                                                                                                      gcatcggcacgtttctttgaagacgacgacggcctgcatCTACTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAGTTACGCTCGGTGAT‑GAGCGTCGGTTTcctctgcgtgggcgtgacccgc                                    >  1:301369‑M2/40‑138 (MQ=255)                                                                                                          cggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGc                                >  2:13368‑M2/36‑143 (MQ=255)                                                                                                           ggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACGGTGGCATTTACGATCTGTGAT‑GGTCGGCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCt                               >  2:746273‑M2/35‑143 (MQ=255)                                                                                                                 ttGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTCA‑GATAGGCGGTAGTTTTTCTGTTTACTCTGCGTGAGCGTGAACTGCGCGCTTCTCGTCTGtc                    >  2:199904/1‑142 (MQ=255)                                                                                                                     tttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGt                     <  2:257185‑M2/119‑1 (MQ=255)                                                                                                                        gaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATc                  <  2:816982‑M2/122‑1 (MQ=255)                                                                                                                        gaagacgacgacggcctgcagCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACGCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATc                  <  2:587506‑M2/122‑1 (MQ=255)                                                                                                                             cgacgacggcctgcatCTCCTTCTTCTTCTTCGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGCGCGTGAACTCCCCCCTTCTCGTCTGTATCgtagg             >  1:153663‑M2/17‑141 (MQ=255)                                                                                                                                 gacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTg         <  1:472391‑M2/131‑1 (MQ=255)                                                                                                                                  ccggcctgcctCTCCTTCTTCTTCTTTGAAGATGCGGAAGTTCACGCCGGTAACGCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGc        <  1:824473‑M2/132‑1 (MQ=255)                                                                                                                                        tgcatCTCCTTCTTCTGCTTTGAGGAGGCGGAAGATCCCGCCGGTTACTCCTCTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAg  <  1:250412‑M2/138‑1 (MQ=255)                                                                                                                                             |                                                                                                                                           ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT‑GGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAG  >  NC_000913/4001509‑4001786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.