Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 4,001,644 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40016431 (0.050)20 (0.940) 19/284 0.1 95.2% coding (219/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913 4001649 = 1 (0.050)coding (225/951 nt) corA magnesium/nickel/cobalt transporter

TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT  >  NC_000913/4001514‑4001774
                                                                                                                                       |                                                                                                                             
attgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggcactggacgacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCattct                                                                                                                        >  1:60667‑M2/136‑143 (MQ=255)
   tatcttgtcgacccgaccgactgcgtgcgcctgcgcgtacaatctcaacttggccagagcctgtcatcccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagtcgacgacggcctgcatCTCcttcttct                                                                                                                     <  2:350974‑M2/11‑1 (MQ=255)
                cggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                        <  2:46276‑M2/24‑1 (MQ=255)
                 ggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagccatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTCACGATGCg                                                                                                       >  1:194073‑M2/119‑143 (MQ=255)
                                  tgcgcgtagtgtgtgagcttggccagagcctggcaaccggcccggaactggaagacatcgaggcgtcggcacgtttctttgaagacgacgacggcgtgcatCTCCTTGTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTaa                                                                                      <  2:273131‑M2/42‑1 (MQ=255)
                                  ggcgcgtacactcgcaagctggcccgagcctggcaacccgcgcggaactagaagacgtcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTaa                                                                                      <  2:273041‑M2/42‑1 (MQ=255)
                                                 aacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAtt                                                                       <  2:10708‑M2/57‑1 (MQ=255)
                                                       gccgcggcctggcgtcccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgaccgcctgcacCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCAt                                                                 <  2:294990‑M2/63‑1 (MQ=255)
                                                                      cccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTATTCATTAAAGATGAAGAAGAACACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCt                                                  >  1:289730‑M2/66‑143 (MQ=255)
                                                                         gcccgcaacttgaagacatcgtagcatcggcacgtttctttgaagacgactacggcctgcatTTCTTTTTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACCCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                               <  1:174292‑M2/81‑1 (MQ=255)
                                                                                  tggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTCGAAGCTGCGGAAGATCACGCCGGCAACCCCACTGTGCCATCTACCATCCCGGATGCTCGTctgtctcctccccg                                      >  2:171291‑M2/54‑139 (MQ=255)
                                                                                          atcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCTGTGATTGTAAACTGTTTACTCTgcgtgagcgtg                              >  1:5328‑M2/46‑143 (MQ=255)
                                                                                            cgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTTCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGaa                            <  2:359505‑M2/100‑1 (MQ=255)
                                                                                                    cGTTTCTTTGAAGGAGACGACGCCCTGC‑‑‑‑‑CTCTCCTTCTTCTTCTTTGACGATGCGGAAGATCACGCCGGTAACTCCTCTGTGGTATTTACCATCCGTGATGGTCGTCTGTTTTCTCTGCGTGAGCGTGTACTGCCCGCTTTTc               <  2:280092/143‑1 (MQ=255)
                                                                                                      gcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGACTGTATACTCTGAGAGAGCATGAACTACAcgcat                  >  1:347562‑M2/34‑141 (MQ=255)
                                                                                                           tttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCGGGTAACCCCACGGTGGCGTTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGGTCTGCCCGCCGTTcgg             >  2:83226‑M2/29‑142 (MQ=255)
                                                                                                            ttctgtgaaggcgacgacggcctgcatGTCCTTCTTCTTCTTGGAAGATGCGGAAGAGCACGCCGGTAACTCCACTTGGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTc            <  2:351671‑M2/116‑1 (MQ=255)
                                                                                                               tttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGCAGTCTGTTTACTCGCCGTGAGAGTGAACTGCCCGCGTCTCGCCTGt         >  2:316668‑M2/25‑143 (MQ=255)
                                                                                                                  gaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGTCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATc      <  1:281678‑M2/122‑1 (MQ=255)
                                                                                                                     ggcgacgacggcctgcatTTCTTTGTTTTTCTTTGAAGATTCGGAAGATTACGCGGGTAACTCCACTGTGGGATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa   <  1:158527‑M2/125‑1 (MQ=255)
                                                                                                                      acgacgacggcctgcatCTCCTTCTTCTTCTTTGATGATGCGGAAGATCACTCTGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAt  <  1:235620‑M2/126‑1 (MQ=255)
                                                                                                                                       |                                                                                                                             
TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT  >  NC_000913/4001514‑4001774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.