Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,978,503 Δ776 bp insB1insA insB1, insA

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978503–1979269 1979278 10–776 63 [1] [0] 64 insB1–insA insB1, insA

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 19785021 (0.010)57 (0.890) 43/266 0.5 99.2% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein

GTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCG  >  NC_000913/1978363‑1978643
                                                                                                                                           |                                                                                                                                             
gTCTTGTCACGTAGCTAAGATTTTTCGTAAATCGACGCAAGTTTACTCGTCACTACACTCTCATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                            <  2:776640‑M1/143‑4 (MQ=255)
  cTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                          <  1:9272‑M1/143‑6 (MQ=255)
  cTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                          <  1:772686‑M1/143‑6 (MQ=255)
             tCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttt                                                                                                                               >  2:289592‑M1/1‑127 (MQ=255)
               tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGGTTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgt                                                                                                                             >  1:746245‑M1/1‑125 (MQ=255)
               tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATACATGtatacgtcatacttttgt                                                                                                                             >  1:442108‑M1/1‑125 (MQ=255)
                 aGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtactacttttgttt                                                                                                                           >  2:1080492‑M1/1‑123 (MQ=255)
                      tttCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                                                                      <  1:119272‑M1/143‑26 (MQ=255)
                        tCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATCCTGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATACATGtataagtcatacttttgttttgggtgt                                                                                                                    <  2:77948‑M1/143‑28 (MQ=255)
                           tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAACTGtataagtcatacttttgttttgggtgtatt                                                                                                                 >  2:454184‑M1/1‑113 (MQ=255)
                           tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatt                                                                                                                 >  1:132009‑M1/1‑113 (MQ=255)
                            aaaTCGACGCTACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCGTATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtattt                                                                                                                <  2:438608‑M1/143‑32 (MQ=255)
                            aaaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtattt                                                                                                                <  1:643800‑M1/143‑32 (MQ=255)
                            aaaTCGACGCAACTGGACTCGTCACTACACGCACATAGAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACATTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtattt                                                                                                                <  2:748648‑M1/143‑32 (MQ=255)
                              aTCGACGCACCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttca                                                                                                              <  2:1105107‑M1/143‑34 (MQ=255)
                              aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttca                                                                                                              >  2:1173776‑M1/1‑110 (MQ=255)
                                     cAAGTGCACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAGTGCGTGATGCATTTCGCATAGACCATTTAATTACTTATTATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtt                                                                                                       <  2:1032433‑M1/143‑41 (MQ=255)
                                      aaCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtta                                                                                                      >  1:342075‑M1/1‑102 (MQ=255)
                                      aaCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTCTCAATAATGCGTGATGCAGATCACACAAAACACTCAGTGACGTACCATAAATTtataaggccgacttctgttatgagtctatctcaatctgtta                                                                                                      >  1:546989‑M1/1‑102 (MQ=255)
                                         tctacTCGACACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagacatacttttgttttgggtgtatttcaatctgttaaaa                                                                                                   <  2:1112078‑M1/141‑45 (MQ=255)
                                         tGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaa                                                                                                   >  1:491119‑M1/1‑99 (MQ=255)
                                         tGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaa                                                                                                   >  1:649791‑M1/1‑99 (MQ=255)
                                         tGTACTCGTCACTACACGCACATACAACGGAGGCGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttaggtgtatttcaatctgttaaaa                                                                                                   >  2:7100‑M1/1‑99 (MQ=255)
                                                     tACAGGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAACTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcta                                                                                       <  2:764946‑M1/143‑57 (MQ=255)
                                                          gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtatacgtcatacttttgatttgggtgtctttcaatctggtaaaaagtttttcgctacgcta                                                                                  >  2:268260‑M1/1‑82 (MQ=255)
                                                              aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                                                              <  1:686114‑M1/143‑66 (MQ=255)
                                                              aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtattgggtgtatttcaatctgttaaaaagtttttcgctacgctcaaac                                                                              >  2:402103‑M1/1‑78 (MQ=255)
                                                                 cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttccatctgttaaaacgtttttcgctacgatagcaagca                                                                           >  1:97375‑M1/1‑75 (MQ=255)
                                                                 cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGACGCAGATCACACAAAACACTCAATTACTTAACATAAATGtatcagtcatacttttgttcggggtgtatctccatctgttaacaagagtttcgctacgctcgcaaacg                                                                           >  1:97306‑M1/1‑75 (MQ=255)
                                                                  aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGCTGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggatgtatttcaatctgtgaaaaagtttttcgctaccctagcacgcca                                                                          >  2:1029106‑M1/1‑74 (MQ=255)
                                                                  aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAACTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctccgctagcaagcaa                                                                          >  1:467972‑M1/1‑74 (MQ=255)
                                                                    cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                                                                        <  2:489455‑M1/143‑72 (MQ=255)
                                                                    cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtggatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                                                                        >  1:741467‑M1/1‑72 (MQ=255)
                                                                     ggAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATCtataagtcatacttttgttttgggtgtatttcagtctgttaaaaagtttttcgctacgctcgcaagcaaaca                                                                       >  1:82768‑M1/1‑71 (MQ=255)
                                                                     ggAGGGGGGCTGCGATTTTCAATAATGAGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtctttcgctaggctagcaagcaaaaa                                                                       >  2:733865‑M1/1‑71 (MQ=255)
                                                                      gAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaat                                                                      <  1:1173776‑M1/143‑74 (MQ=255)
                                                                         gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaa                                                                   >  2:287591‑M1/1‑67 (MQ=255)
                                                                          agggcTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaagctgttaaaaagtttttcgctacgctagaaagcaaaaatgaaa                                                                  <  2:913324‑M1/142‑78 (MQ=255)
                                                                             gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAGATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                               <  2:584177‑M1/143‑81 (MQ=255)
                                                                               tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagga                                                             >  1:506282‑M1/1‑61 (MQ=255)
                                                                                 cGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaat                                                           <  1:1011897‑M1/143‑85 (MQ=255)
                                                                                          ataacGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat                                                  <  1:187487‑M1/143‑94 (MQ=255)
                                                                                                      gCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgc                                      >  1:560551‑M1/1‑38 (MQ=255)
                                                                                                         gATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                   >  2:674897‑M1/1‑35 (MQ=255)
                                                                                                         gATCACACAAAACACTCAATTACTTAACATAAATGtataagacatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                   >  2:1019611‑M1/1‑35 (MQ=255)
                                                                                                          aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacag                                  >  2:330485‑M1/1‑34 (MQ=255)
                                                                                                             acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtca                               <  1:728483‑M1/143‑113 (MQ=255)
                                                                                                               acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagccagcaaaaatgaaccaggaataatagaaatgggatgttgcgcacagtcaaa                             >  1:678385‑M1/1‑29 (MQ=255)
                                                                                                                caAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                            >  1:605384‑M1/1‑28 (MQ=255)
                                                                                                                 aaaaCTCTTAATTTTTTAATATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacatgaataatcgaaatgggatgttgcgcacagtcaaaat                           <  2:82768‑M1/143‑117 (MQ=255)
                                                                                                                  aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaata                          >  1:15771‑M1/1‑26 (MQ=255)
                                                                                                                   aaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatggtgcgcgcaatcaagagtg                         >  2:828707‑M1/1‑25 (MQ=255)
                                                                                                                          aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaacaatgacacaggaataatcgaaatggaacattgcgcacagtcaaaataactgaccg                  >  2:190936‑M1/1‑18 (MQ=255)
                                                                                                                          aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgacacagcaatcatcgaaatgggatgttgcacacagtcaaaataactaacag                  >  1:239998‑M1/1‑18 (MQ=255)
                                                                                                                          aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccg                  >  1:4352‑M1/1‑18 (MQ=255)
                                                                                                                           agtagttaacatAAATGtatgagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgt                 <  2:232703‑M1/138‑127 (MQ=255)
                                                                                                                                ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaata            >  1:1119537‑M1/1‑12 (MQ=255)
                                                                                                                                     aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcat       >  1:292648‑M1/1‑7 (MQ=255)
                                                                                                                                       atgtGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATg     <  2:292271/140‑1 (MQ=1)
                                                                                                                                         aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc   <  2:342075‑M1/143‑141 (MQ=255)
                                                                                                                                         aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc   <  1:330485‑M1/143‑141 (MQ=255)
                                                                                                                                          tGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcagcgtaaataatcatctgct  >  1:196793‑M1/1‑2 (MQ=255)
                                                                                                                                          tGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgct  <  1:544509‑M1/143‑142 (MQ=255)
                                                                                                                                           |                                                                                                                                             
GTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCG  >  NC_000913/1978363‑1978643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.