Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	60 [0]	[0] 59	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	56 (0.850)	46/274	0.4	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCT  >  NC_000913/1299356‑1299639                                                                                                                                               |                                                                                                                                              aaaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                                                                                                                               >  2:193907‑M1/1‑143 (MQ=255)         gttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGCATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCGAAataataat                                                                                                                                       >  2:449877‑M1/1‑135 (MQ=255)            tgttatAAGGATTTCCTTAACTGCTTCTCCTCACCGTCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                                                                                                                    <  2:616970‑M1/142‑12 (MQ=255)              ttaTAAGGCTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCCTAATCCTGGCCTTCCTGACGAATAATTGAAATGATATTATTAATTCCCCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                  >  2:461179‑M1/1‑130 (MQ=255)              ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                  <  2:1062435‑M1/143‑14 (MQ=255)                ataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgc                                                                                                                                >  2:650968‑M1/1‑128 (MQ=255)                 taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                               >  1:307445‑M1/1‑127 (MQ=255)                    ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGGAAGGTGTTTTGGTAGataataatcaattgttaaa                                                                                                                            >  1:918131‑M1/1‑124 (MQ=255)                      aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTCGGCTTGCTGCAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataacaatcaattcttaaatc                                                                                                                          >  2:963804‑M1/1‑122 (MQ=255)                         tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                       <  1:228373‑M1/143‑25 (MQ=255)                         tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                       <  1:247760‑M1/143‑25 (MQ=255)                            ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATAATATTATTAATTCCACTACCTTTGATATATGAAAATGCTAAataataatcaatagttaaattattttg                                                                                                                    >  2:74221‑M1/1‑116 (MQ=255)                                   cTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                             <  1:123184‑M1/143‑35 (MQ=255)                                   cTTCTCCTCACCATCATGTTATTTTCGCCACAGCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                             <  1:491517‑M1/143‑35 (MQ=255)                                        cctcACCATCATGTTATTTTCGCCGCATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact                                                                                                        >  1:873565‑M1/1‑104 (MQ=255)                                         ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                       >  1:1057979‑M1/1‑103 (MQ=255)                                              catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                  >  2:202534‑M1/1‑98 (MQ=255)                                                 catGTTATTTTCGCCACATCATACTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATCAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAaatataatcaattgttaaattattgtgcatttcactactggaactgta                                                                                               <  1:840298‑M1/143‑49 (MQ=255)                                                   tGTTATTTGCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                             <  2:157593‑M1/143‑51 (MQ=255)                                                   tGTTATTGTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                             <  1:176315‑M1/143‑51 (MQ=255)                                                    gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                            >  1:889890‑M1/1‑92 (MQ=255)                                                    gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                            >  1:84577‑M1/1‑92 (MQ=255)                                                    gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATAAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                            >  1:79929‑M1/1‑92 (MQ=255)                                                     ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                           >  1:1095125‑M1/1‑91 (MQ=255)                                                        ttgtcgcCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGACGAAAGTGCTAAataataatcaattgttaaattattgatcatttcactactagaactataatcagaa                                                                                        >  2:1135261‑M1/4‑88 (MQ=255)                                                         tttCGTCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                       <  2:1031362‑M1/143‑57 (MQ=255)                                                               cacaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                 <  1:477655‑M1/143‑63 (MQ=255)                                                               cacaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaccactgaaactgtaatcagaaaagatag                                                                                 >  2:445200‑M1/1‑81 (MQ=255)                                                                acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                <  1:682467‑M1/143‑64 (MQ=255)                                                                acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                >  1:233415‑M1/1‑80 (MQ=255)                                                                  atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagaca                                                                              <  1:983920‑M1/143‑66 (MQ=255)                                                                    catAATCCTGCGCTTGCGTAAGAATAATTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                            <  1:189841‑M1/143‑68 (MQ=255)                                                                     atAATCCTGGGCTTGCTGGAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                           <  1:218339‑M1/143‑69 (MQ=255)                                                                         tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                       >  2:741333‑M1/1‑71 (MQ=255)                                                                              ggCTTGCGGAAGAATAATTGAAATGAGATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaat                                                                  <  2:8943‑M1/143‑78 (MQ=255)                                                                                     tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactataatcagaaaagatagacatgcatagccactctcgatt                                                           >  2:911517‑M1/1‑59 (MQ=255)                                                                                      gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt                                                          <  1:837090‑M1/143‑86 (MQ=255)                                                                                      gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt                                                          <  1:193907‑M1/143‑86 (MQ=255)                                                                                        agaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttga                                                        <  2:119782‑M1/143‑88 (MQ=255)                                                                                        agaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttga                                                        <  2:647208‑M1/143‑88 (MQ=255)                                                                                          aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                      >  2:590232‑M1/1‑54 (MQ=255)                                                                                             aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaa                                                   <  1:1137836‑M1/143‑93 (MQ=255)                                                                                              actcatgagatattattAGTGCCACCGCCTCTGGTAGAGGAAAGTGCTACataataatcaattgtcaaattattgtgcacttcactactggaactgtaatcagaaaagatagacatgcgtagccaatctctatttgattgaat                                                  <  1:874944‑M1/135‑94 (MQ=255)                                                                                                  aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgagtgacttgaa                                              >  2:1076743‑M1/1‑46 (MQ=255)                                                                                                   aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                             <  1:217266‑M1/143‑99 (MQ=255)                                                                                                       atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatg                                         >  2:541039‑M1/1‑41 (MQ=255)                                                                                                         attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagctagacatgcttagccaatatctatttgattgaactgaaagatgtt                                       >  1:384529‑M1/1‑39 (MQ=255)                                                                                                         attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtt                                       >  1:384534‑M1/1‑39 (MQ=255)                                                                                                         attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtt                                       >  1:384535‑M1/1‑39 (MQ=255)                                                                                                         attagtAATTCCACGGGCTTTGGTAGAGGAAAGTGCTAAataataatcaagtgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtt                                       <  2:1119257‑M1/143‑105 (MQ=255)                                                                                                               aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                 <  2:1183593‑M1/143‑111 (MQ=255)                                                                                                                aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtacatcgtactaagagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                >  1:547145‑M1/1‑32 (MQ=255)                                                                                                                 ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                               >  1:189734‑M1/1‑31 (MQ=255)                                                                                                                    cACTGCCTTTGGTCGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                            <  2:1095125‑M1/143‑116 (MQ=255)                                                                                                                    cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                            >  1:412047‑M1/1‑28 (MQ=255)                                                                                                                           tttGGTAGAGGAAAGTGCTACataataatcaattgttaaattattgtgcatttaacaactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgca                     >  1:345402‑M1/1‑21 (MQ=255)                                                                                                                            ttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                    <  1:706152‑M1/143‑124 (MQ=255)                                                                                                                                            cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgata    <  2:1057979‑M1/143‑140 (MQ=255)                                                                                                                                             tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgagttgaaagatgtgtgttaaggcatggatgcaagctatagattctgatag   >  1:726923‑M1/1‑3 (MQ=255)                                                                                                                                              aaataataatcaattgttaaattattgtgcatttcaccactggaactgtaatcagaaaagatagacatgcttagccggtcgctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg  <  1:445200‑M1/143‑142 (MQ=255)                                                                                                                                               |                                                                                                                                              AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCT  >  NC_000913/1299356‑1299639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑