Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 1,159,286 +GTA coding (1418/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 11592821 (0.020)59 (1.220)
+AGT
51/268 0.0 98.3% coding (1414/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component
?NC_000913 1159283 = 1 (0.020)coding (1415/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component

TGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATG‑‑‑AGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC  >  NC_000913/1159142‑1159420
                                                                                                                                            |                                                                                                                                             
tGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGag                                                                                                                                             >  2:399485‑M1/1‑141 (MQ=255)
  cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagta                                                                                                                                           >  1:53646‑M1/1‑139 (MQ=255)
    cgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGTCCTGAAGAAGCTGGGCGCAGCGGGCGTAGTGGTCGCTGGTTCTGGTGTGCAGGCGATTTGCGGTACTAAAGCCGATAACCTGAAAACCGAGATGGATGagtagt                                                                                                                                         <  1:195309‑M1/143‑7 (MQ=255)
           gTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccg                                                                                                                                  <  2:832639‑M1/143‑14 (MQ=255)
            ttGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgt                                                                                                                                 >  1:701706‑M1/1‑129 (MQ=255)
                tGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaacc                                                                                                                             >  2:465459‑M1/1‑125 (MQ=255)
                  aTGTGGCTAGAGTGGGTCAGGGCGTCCGGGGGGGACTGGGCGCGTCGGGCGTAGTGGTTGCTGGTTCTGCTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccac                                                                                                                           <  1:304583‑M1/143‑21 (MQ=255)
                     tgtCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTACATCCGATAACCTGAAAACCGACATGGATGagtagtacatccgtacccactac                                                                                                                        >  2:388352‑M1/1‑120 (MQ=255)
                            aGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaa                                                                                                                 <  2:53646‑M1/143‑31 (MQ=255)
                            aGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaa                                                                                                                 <  2:321926‑M1/143‑31 (MQ=255)
                             gTGGATCAGGCCGGCCTGAAGAAACTGGGCGCCGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGGGTTTCGGTACTAAATCCGATAACCTGAAAGCCGAGATGGATGagtagtacatccgtgaccactaatccgtaac                                                                                                                >  2:564535‑M1/1‑112 (MQ=255)
                                  tCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTAAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgtc                                                                                                           >  1:430367‑M1/1‑107 (MQ=255)
                                       ccggccTGAAGAAACTGGGCGCAGCGTGCGTCGTGGTGGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAAGCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttgggga                                                                                                      <  1:50900‑M1/143‑42 (MQ=255)
                                         ggccTGAAGACACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggaga                                                                                                    <  2:222040‑M1/143‑44 (MQ=255)
                                            cTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagacta                                                                                                 >  2:476037‑M1/1‑97 (MQ=255)
                                            cTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgtgggggagactc                                                                                                 >  2:179150‑M1/1‑97 (MQ=255)
                                              gaaggaACTGGACACCGAGGTGGTGGTGGTTGCTGGTTCTGCTGTTCACGCGCTTCACGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGattagtacatccgtaaccactaatccgtaagacgttggggagactaag                                                                                               <  2:663805‑M1/143‑49 (MQ=255)
                                              gaagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatctgtaaccactaatccgtaagacgttggggagactaag                                                                                               <  2:557723‑M1/143‑49 (MQ=255)
                                              aaagaggctgggcgcAGCGGGCGTAGTGGTTGCTGGTTGTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaag                                                                                               <  1:829677‑M1/136‑49 (MQ=255)
                                                   aaCTGGGCGCAGCTGGCTTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagc                                                                                          <  2:266299‑M1/143‑54 (MQ=255)
                                                     cTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagcca                                                                                        >  2:110469‑M1/1‑88 (MQ=255)
                                                        ggCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagat                                                                                     <  2:327536‑M1/143‑59 (MQ=255)
                                                           gcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGATCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGGGATGGATGagaagtacatccgtcaccactaatccgtaagacgatgcgaagactaaggcaaccagatgac                                                                                  >  1:225247‑M1/1‑82 (MQ=255)
                                                            cagggtgcgtagtGGTTGCTGGTTCTGGTGTTCGGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggct                                                                                 <  1:389239‑M1/137‑63 (MQ=255)
                                                            cAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggct                                                                                 >  2:477679‑M1/1‑81 (MQ=255)
                                                               cGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTATCGGTATAATACCGGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccatctggctgcc                                                                              <  1:646692‑M1/143‑66 (MQ=255)
                                                                  gCGTAGTGGTTGCCGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccatctggctgcctta                                                                           <  2:438648‑M1/143‑69 (MQ=255)
                                                                      aGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgcctttttta                                                                       <  2:366886‑M1/143‑73 (MQ=255)
                                                                       gtcgttgCTGGTTCTGGTGGTCAGTCGATGCTCGGTACTAAATCCGATATCCTTAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaatgcagccagatggctgccttttttac                                                                      <  1:837100‑M1/140‑74 (MQ=255)
                                                                         tgtTGCTGGCTGTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacag                                                                    <  1:408672‑M1/142‑76 (MQ=255)
                                                                            tGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaacgactaatccgtaagacgttggggagaataaggcagccagatggctgccacatgtacaggtg                                                                 >  1:742063‑M1/1‑65 (MQ=255)
                                                                            tGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtg                                                                 <  2:414833‑M1/143‑79 (MQ=255)
                                                                              cTGGTTCTGGTGTTCAGGCGAGTTTCGGTACTAAATCCGAGAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgtt                                                               <  1:745105‑M1/143‑81 (MQ=255)
                                                                               tGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacctccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttac                                                                      <  2:362975‑M1/135‑74 (MQ=255)
                                                                               tGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttac                                                                      >  1:362975‑M1/1‑62 (MQ=255)
                                                                                   gctcgtgttcagGCGATTGTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaagccgtaagaggtcgaggagactaaggcagccagatggctgccttttttacaggtgttattca                                                          <  1:184846‑M1/139‑86 (MQ=255)
                                                                                    cTGGTGTTCAGGCGATTTTCGGGACTAAAGCCGATAACCTGAAAACCGAGATGGATGagtaggacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcag                                                         <  2:434871‑M1/143‑87 (MQ=255)
                                                                                     tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcaga                                                        >  2:697727‑M1/1‑56 (MQ=255)
                                                                                        tgtTCAGGCGATGTTCTGTACTAAATCCGATAACCTGAAAACTGAGATGGATGattagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaatt                                                     <  1:13485‑M1/143‑91 (MQ=255)
                                                                                         gtTCAGGCGATTTTCGGTACTAAATCCGATAACGTGAAAACCGAGATGGATGagtagtacatccgtaacaactaatccgtaagacgttggggagactaaggcagccagatggcggccttttttacaggtgtgattcagaattg                                                    >  2:740502‑M1/1‑52 (MQ=255)
                                                                                                 gATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATAGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttaccggtgttgttcagaattgatacgtgc                                            >  2:723073‑M1/1‑44 (MQ=255)
                                                                                                   ttttCGGTACGAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccg                                          <  2:826287‑M1/143‑102 (MQ=255)
                                                                                                         gTACTAAGTCCGATCACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaacgcagccagatggctgccttgtttacaggtgttattcagaattgatacgtgccggtaatg                                    <  1:594783‑M1/143‑108 (MQ=255)
                                                                                                         gTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatg                                    <  2:701706‑M1/143‑108 (MQ=255)
                                                                                                         gTACTAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgatggggagactaagacagccagatggctgacttatttacaggtgttattcagcattgatacgtgccggtaatg                                    >  1:86080‑M1/1‑36 (MQ=255)
                                                                                                           acgaaagccGATAGCCTGAAACCAGCGATGGATGagtagtacatccgtaaccactaagccgtaagacgttggggaggcgaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgct                                  <  2:268172‑M1/140‑110 (MQ=255)
                                                                                                             tAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgctga                                <  1:110469‑M1/143‑112 (MQ=255)
                                                                                                             tAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgctga                                <  2:643222‑M1/143‑112 (MQ=255)
                                                                                                             tAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatg                                                                                    >  2:373597‑M1/1‑32 (MQ=255)
                                                                                                             tAAATCCGATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatg                                                                                    <  1:373597‑M1/91‑60 (MQ=255)
                                                                                                                aTCGGATAACCGGAAAACCGATAGGGATGggtagtacctccgtaaccactgatccgtaaggcgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtattgctgaaat                             <  1:65377‑M1/143‑115 (MQ=255)
                                                                                                                    gATAACCTGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgcctttgttacaggtgttattcagaattgatacgtaccggggatgctgaaattacg                         >  1:640487‑M1/1‑25 (MQ=255)
                                                                                                                      gaacCTGAAAACCGCGATGGATGagtagtacatccgtaaccgctaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgctgaaattacgcg                       <  2:594155‑M1/142‑121 (MQ=255)
                                                                                                                           tGAAAACCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtggcggtaatgctgaaattacgcggtgtg                  >  2:459529‑M1/1‑18 (MQ=255)
                                                                                                                           tGAAAACCGAGATGGATGAGTAGTACATCCGTAACCACGAATCCGTAAGACGGAGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACGGGTGTTATTCAGAATGAATCCGTGCGGGTGCTGCTGAGATCAcagactgtg                  >  1:436185/1‑135 (MQ=255)
                                                                                                                              aaaCCGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgctgaaattacgcggtgtgccg               >  2:310056‑M1/1‑15 (MQ=255)
                                                                                                                                  cGAGATGGATGagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgctgaaattacgcggtgtgccgtaga           <  1:476037‑M1/143‑133 (MQ=255)
                                                                                                                                  cGAGATCGATGaggagtacccccgccacaactaatatgtaacacattggggacactaaggccgccagatggctgccttttttacaggcgttattcagaattgatacgtgccggtaatgctgaaattacgcggtgtgccgtaga           <  1:179150‑M1/143‑133 (MQ=255)
                                                                                                                                   gagtTGTATGagtaggacagtcgtaaacactaatccgtaagaagttggggagattaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgctgaaattacgcggtgtgccgtagac          <  1:219210‑M1/143‑134 (MQ=255)
                                                                                                                                       tggatgagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgctgaaattacgcggtgtgccgtagacgata      <  1:465459‑M1/143‑138 (MQ=255)
                                                                                                                                         gatgagtattccatgcgtaaccgctaatcggtaggtcgtttgggatactaatgcagccagatggctgccttgtttacgggtgttattcagaattgatacgtgccggtaatgctgaaattacgcggtgtgccgtagacgataga    <  1:796947‑M1/143‑140 (MQ=255)
                                                                                                                                          atgagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatacgtgccggtaatgctgaaattacgcggtgtgccgtagacgatagaa   <  2:757652‑M1/143‑141 (MQ=255)
                                                                                                                                           tgagtagtacatccgtaaccactaatccgtaagacgttggggagactaaggcagccagatggctgccttttttacaggtgttattcagaattgatccgtgccggtagtgctgaaattacgcggtgtgccgtagacgatagaac  >  2:274880‑M1/1‑2 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATG‑‑‑AGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC  >  NC_000913/1159142‑1159420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.