Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 1,159,286 +GTA coding (1418/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 11592821 (0.020)59 (1.220)
+AGT
51/268 0.0 98.3% coding (1414/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component
?NC_000913 1159283 = 1 (0.020)coding (1415/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component

CGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATG‑‑‑AGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC  >  NC_000913/1159144‑1159420
                                                                                                                                              |                                                                                                                                         
cgcgtcagcgttgctgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagta                                                                                                                                           >  1:53646‑M2/143‑143 (MQ=255)
  cgtcagcgttgctgatgtgtctaaagtggatcaggccgtcctgaagaagctgggcgcagcgggcgtagtggtcgctggttctggtgtgcaggcgatttgcggtactaaagccgataacctgaaaaccgagatggatgagtagt                                                                                                                                         <  1:195309‑M2/3‑1 (MQ=255)
         gttgctgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCg                                                                                                                                  <  2:832639‑M2/10‑1 (MQ=255)
          ttgctgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGt                                                                                                                                 >  1:701706‑M2/133‑143 (MQ=255)
              tgatgtgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAAcc                                                                                                                             >  2:465459‑M2/129‑143 (MQ=255)
                atgtggctagagtgggtcagggcgtccgggggggactgggcgcgtcgggcgtagtggttgctggttctgctgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCAc                                                                                                                           <  1:304583‑M2/17‑1 (MQ=255)
                   tgtctaaagtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactacatccgataacctgaaaaccgacatggatgagtAGTACATCCGTACCCACtac                                                                                                                        >  2:388352‑M2/124‑142 (MQ=255)
                          agtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTaa                                                                                                                 <  2:53646‑M2/27‑1 (MQ=255)
                          agtggatcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTaa                                                                                                                 <  2:321926‑M2/27‑1 (MQ=255)
                           gtggatcaggccggcctgaagaaactgggcgccgcgggcgtagtggttgctggttctggtgttcaggcgggtttcggtactaaatccgataacctgaaagccgagatggatgagtAGTACATCCGTGACCACTAATCCGtaac                                                                                                                >  2:564535‑M2/116‑142 (MQ=255)
                                tcaggccggcctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttaaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACgtc                                                                                                           >  1:430367‑M2/111‑142 (MQ=255)
                                     ccggcctgaagaaactgggcgcagcgtgcgtcgtggtggctggttctggtgttcaggcgattttcggtactaaagccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGga                                                                                                      <  1:50900‑M2/38‑1 (MQ=255)
                                       ggcctgaagacactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGgaga                                                                                                    <  2:222040‑M2/40‑1 (MQ=255)
                                          ctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTa                                                                                                 >  2:476037‑M2/101‑143 (MQ=255)
                                          ctgaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTGGGGGAGActc                                                                                                 >  2:179150‑M2/101‑142 (MQ=255)
                                            gaaggaactggacaccgaggtggtggtggttgctggttctgctgttcacgcgcttcacggtactaaatccgataacctgaaaaccgagatggatgattAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAg                                                                                               <  2:663805‑M2/45‑1 (MQ=255)
                                            gaagaaactgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCTGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAg                                                                                               <  2:557723‑M2/45‑1 (MQ=255)
                                            aaagaggctgggcgcagcgggcgtagtggttgctggttgtggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAg                                                                                               <  1:829677‑M2/45‑1 (MQ=255)
                                                 aactgggcgcagctggcttagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGc                                                                                          <  2:266299‑M2/50‑1 (MQ=255)
                                                   ctgggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCa                                                                                        >  2:110469‑M2/92‑143 (MQ=255)
                                                      ggcgcagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt                                                                                     <  2:327536‑M2/55‑1 (MQ=255)
                                                         gcagcgggcgtagtggttgctggttctggtgatcaggcgattttcggtactaaatccgataacctgaaaaccgggatggatgagaAGTACATCCGTCACCACTAATCCGTAAGACGATGCGAAGACTAAGGCAACCAGatgac                                                                                  >  1:225247‑M2/86‑141 (MQ=255)
                                                          cagggtgcgtagtggttgctggttctggtgttcgggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                 <  1:389239‑M2/59‑1 (MQ=255)
                                                          cagcgggcgtagtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                 >  2:477679‑M2/85‑143 (MQ=255)
                                                             cgggcgtagtggttgctggttctggtgttcaggcgattatcggtataataccggataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGcc                                                                              <  1:646692‑M2/62‑1 (MQ=255)
                                                                gcgtagtggttgccggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCctta                                                                           <  2:438648‑M2/65‑2 (MQ=255)
                                                                    agtggttgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTa                                                                       <  2:366886‑M2/69‑1 (MQ=255)
                                                                     gtcgttgctggttctggtggtcagtcgatgctcggtactaaatccgatatccttaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAATGCAGCCAGATGGCTGCCTTTTTTAc                                                                      <  1:837100‑M2/70‑1 (MQ=255)
                                                                       tgttgctggctgtggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                    <  1:408672‑M2/72‑1 (MQ=255)
                                                                          tgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACGACTAATCCGTAAGACGTTGGGGAGAATAAGGCAGCCAGATGGCTGCCACATGTACAGgtg                                                                 >  1:742063‑M2/69‑143 (MQ=255)
                                                                          tgctggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgtg                                                                 <  2:414833‑M2/75‑1 (MQ=255)
                                                                            ctggttctggtgttcaggcgagtttcggtactaaatccgagaacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGtt                                                               <  1:745105‑M2/77‑1 (MQ=255)
                                                                             tggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACCTCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTAc                                                                      <  2:362975‑M2/70‑1 (MQ=255)
                                                                             tggttctggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTAc                                                                      >  1:362975‑M2/66‑135 (MQ=255)
                                                                                 gctcgtgttcaggcgattgtcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAAGCCGTAAGAGGTCGAGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCa                                                          <  1:184846‑M2/82‑1 (MQ=255)
                                                                                  ctggtgttcaggcgattttcgggactaaagccgataacctgaaaaccgagatggatgagtAGGACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAg                                                         <  2:434871‑M2/83‑1 (MQ=255)
                                                                                   tggtgttcaggcgattttcggtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGa                                                        >  2:697727‑M2/60‑143 (MQ=255)
                                                                                      tgttcaggcgatgttctgtactaaatccgataacctgaaaactgagatggatgattAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAAtt                                                     <  1:13485‑M2/87‑1 (MQ=255)
                                                                                       gttcaggcgattttcggtactaaatccgataacgtgaaaaccgagatggatgagtAGTACATCCGTAACAACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCGGCCTTTTTTACAGGTGTGATTCAGAATTg                                                    >  2:740502‑M2/56‑143 (MQ=255)
                                                                                               gattttcggtactaaatccgataacctgaaaaccgagatagatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACCGGTGTTGTTCAGAATTGATACGTGc                                            >  2:723073‑M2/48‑143 (MQ=255)
                                                                                                 ttttcggtacgaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCg                                          <  2:826287‑M2/98‑1 (MQ=255)
                                                                                                       gtactaagtccgatcacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAACGCAGCCAGATGGCTGCCTTGTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATg                                    <  1:594783‑M2/104‑1 (MQ=255)
                                                                                                       gtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATg                                    <  2:701706‑M2/104‑1 (MQ=255)
                                                                                                       gtactaaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGATGGGGAGACTAAGACAGCCAGATGGCTGACTTATTTACAGGTGTTATTCAGCATTGATACGTGCCGGTAATg                                    >  1:86080‑M2/40‑143 (MQ=255)
                                                                                                         acgaaagccgatagcctgaaaccagcgatggatgagtAGTACATCCGTAACCACTAAGCCGTAAGACGTTGGGGAGGCGAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCt                                  <  2:268172‑M2/106‑1 (MQ=255)
                                                                                                           taaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATg                                                                                    >  2:373597‑M2/36‑91 (MQ=255)
                                                                                                           taaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATg                                                                                    <  1:373597‑M2/56‑1 (MQ=255)
                                                                                                           taaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGa                                <  1:110469‑M2/108‑1 (MQ=255)
                                                                                                           taaatccgataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGa                                <  2:643222‑M2/108‑1 (MQ=255)
                                                                                                              atcggataaccggaaaaccgatagggatgggtAGTACCTCCGTAACCACTGATCCGTAAGGCGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTATTGCTGAAAt                             <  1:65377‑M2/111‑1 (MQ=255)
                                                                                                                  gataacctgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTGTTACAGGTGTTATTCAGAATTGATACGTACCGGGGATGCTGAAATTAcg                         >  1:640487‑M2/29‑143 (MQ=255)
                                                                                                                    gaacctgaaaaccgcgatggatgagtAGTACATCCGTAACCGCTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgcg                       <  2:594155‑M2/117‑1 (MQ=255)
                                                                                                                         tgaaaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGGCGGTAATGCTGAAATTACGCGGtgtg                  >  2:459529‑M2/22‑143 (MQ=255)
                                                                                                                         tGAAAACCGAGATGGATGAGTAGTACATCCGTAACCACGAATCCGTAAGACGGAGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACGGGTGTTATTCAGAATGAATCCGTGCGGGTGCTGCTGAGATCAcagactgtg                  >  1:436185/1‑135 (MQ=255)
                                                                                                                            aaaccgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCg               >  2:310056‑M2/19‑143 (MQ=255)
                                                                                                                                cgagatggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGa           <  1:476037‑M2/129‑1 (MQ=255)
                                                                                                                                cgagatcgatgaggAGTACCCCCGCCACAACTAATATGTAACACATTGGGGACACTAAGGCCGCCAGATGGCTGCCTTTTTTACAGGCGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGa           <  1:179150‑M2/129‑1 (MQ=255)
                                                                                                                                 gagttgtatgagtAGGACAGTCGTAAACACTAATCCGTAAGAAGTTGGGGAGATTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc          <  1:219210‑M2/130‑1 (MQ=255)
                                                                                                                                     tggatgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATa      <  1:465459‑M2/134‑1 (MQ=255)
                                                                                                                                       gatgagtATTCCATGCGTAACCGCTAATCGGTAGGTCGTTTGGGATACTAATGCAGCCAGATGGCTGCCTTGTTTACGGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGa    <  1:796947‑M2/136‑1 (MQ=255)
                                                                                                                                        atgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGaa   <  2:757652‑M2/137‑1 (MQ=255)
                                                                                                                                         tgagtAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATCCGTGCCGGTAGTGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAc  >  2:274880‑M2/6‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                         
CGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATG‑‑‑AGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAC  >  NC_000913/1159144‑1159420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.