Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 4,001,644 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40016431 (0.020)50 (0.970) 44/284 0.1 98.0% coding (219/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913 4001649 = 1 (0.020)coding (225/951 nt) corA magnesium/nickel/cobalt transporter

TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT  >  NC_000913/4001514‑4001774
                                                                                                                                       |                                                                                                                             
attgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggccggcatctccgtgt                                                                                                                        >  1:341304‑M2/136‑139 (MQ=255)
    atcttgtcgaaccggacgacgacgagcgacggagcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgttgctttgcagacggcgacggcctgcatctcctgcgcctt                                                                                                                    >  2:473309‑M2/132‑136 (MQ=255)
      cttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcgcggcactggaagccatcgaagcatcggcacgtttctttgcagacgacgacggcctgcatCTCcttcttcttct                                                                                                                  >  1:600126‑M2/130‑143 (MQ=255)
          tcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGa                                                                                                              <  1:627497‑M2/18‑1 (MQ=255)
           cgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcatgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGaa                                                                                                             <  1:757827‑M2/19‑1 (MQ=255)
                cggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                        <  1:817438‑M2/24‑1 (MQ=255)
                cggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                        <  1:421015‑M2/24‑1 (MQ=255)
                        gacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgccggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATc                                                                                                >  1:825045‑M2/112‑143 (MQ=255)
                          cgagcgactgcgcgtacaagctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCAc                                                                                              <  1:642697‑M2/34‑1 (MQ=255)
                             gcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggacctggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAGGATCACgca                                                                                           >  1:275363‑M2/107‑142 (MQ=255)
                              cgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                          >  2:488122‑M2/106‑143 (MQ=255)
                                actgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGCAGATCACGCCGGt                                                                                        >  2:789311‑M2/104‑143 (MQ=255)
                                actgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGt                                                                                        >  1:240836‑M2/104‑143 (MQ=255)
                                  tgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtgtctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGtag                                                                                      >  2:130419‑M2/102‑142 (MQ=255)
                                    cgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcaagtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGACGATGCGGAAGATCACGCCGGTCacc                                                                                    >  1:272193‑M2/100‑142 (MQ=255)
                                     gggtacaatctgagcgtggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcaggtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTc                                                                                   <  2:734731‑M2/45‑1 (MQ=255)
                                     gcgtccaatcagcactcggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTc                                                                                   <  1:770905‑M2/45‑1 (MQ=255)
                                        tacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtatctttgaagacgacgaccgcctgcatCTCCTTCTTCTTCTTTGAAAATGCGGGAGATCACGCCGATAGCTCTAc                                                                                >  2:737959‑M2/96‑143 (MQ=255)
                                           aatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggaacggttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgt                                                                             >  1:258179‑M2/93‑143 (MQ=255)
                                            atctgaacttggccagcgcatcgcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgtg                                                                            <  2:829926‑M2/52‑1 (MQ=255)
                                            atctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgtg                                                                            >  1:621054‑M2/92‑143 (MQ=255)
                                             tctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTgg                                                                           <  2:195689‑M2/53‑1 (MQ=255)
                                             tctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTgg                                                                           <  1:126351‑M2/53‑1 (MQ=255)
                                             tctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACCGTgg                                                                           >  1:578875‑M2/91‑143 (MQ=255)
                                             tctgaacgtggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTgg                                                                           <  1:128218‑M2/53‑1 (MQ=255)
                                               tgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgttactttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTCACTCCACTGTGGCa                                                                         >  1:390117‑M2/89‑143 (MQ=255)
                                                gaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagaccacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAt                                                                        <  1:441019‑M2/56‑1 (MQ=255)
                                                         cagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATcc                                                               >  1:718156‑M2/79‑143 (MQ=255)
                                                             gcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGa                                                           <  2:33776‑M2/69‑1 (MQ=255)
                                                              cctggcaacccgcccggaactggaagacatcgaagcatcggcatctttctttgtagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAt                                                          <  2:188024‑M2/70‑1 (MQ=255)
                                                               ctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATg                                                         <  1:513726‑M2/71‑1 (MQ=255)
                                                                tggcatcccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATccgtgcggg                                                        >  2:326543‑M2/72‑139 (MQ=255)
                                                                 ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGCAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTCCCATCACTGatgca                                                       >  2:31361‑M2/71‑141 (MQ=255)
                                                                       ccgcccggaactggaagacatcgaagcatcgacacgtttctttgaagacgacgccggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGCAACCACAATGTGGCATTTACCATCCGTGATGGTCGTATg                                                 >  1:853267‑M2/65‑143 (MQ=255)
                                                                           ccggatctggaagacctcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTa                                             <  1:303006‑M2/83‑1 (MQ=255)
                                                                            cgggactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAc                                            <  1:555826‑M2/84‑1 (MQ=255)
                                                                                actggaagacatcggtgcatcggcacgtttctgtgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCGGGTAACTCCACTGTGGCACTTACCATCCGTGATGGTCGTCTGTTTACTCTg                                        <  2:474627‑M2/88‑1 (MQ=255)
                                                                                 gtggaagacatcgaagcagcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                       <  1:824241‑M2/89‑1 (MQ=255)
                                                                                   gcaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgt                                     <  1:635586‑M2/91‑1 (MQ=255)
                                                                                    gaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGCAGATGCGGAAGCTCACGCCGGTGACTCCACTGTGGCATTTAGCAGCCGTGATGGTCGTCGGTTTACTctgcgcg                                    >  1:296441‑M2/52‑141 (MQ=255)
                                                                                    gaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTACCTCCACTGTGTCATTTCCCATCCGTGATGGTCGTCTGTTTACTGTgcgtg                                    >  1:588684‑M2/52‑143 (MQ=255)
                                                                                     aTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGACCTGCAT‑‑‑‑‑CTCCATCTTCTACTTTGAACATCCGAACCCTCACGCCAGTAACGCCCCTCTGCTACTTACAATCCGTAGTGGTCGCGTGTTCACCCTcagtgagcgtg                              >  2:317306/1‑143 (MQ=255)
                                                                                         cagcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagcgt                               <  1:460223‑M2/97‑1 (MQ=255)
                                                                                          atcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagcgtg                              >  1:490355‑M2/46‑143 (MQ=255)
                                                                                            cgacgtatcgggaggtttctttgacgacggcgacggcctgcatCGCCTGCTTCTTCTTGGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAGTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGaa                            <  2:349218‑M2/100‑1 (MQ=255)
                                                                                                          gtttctttgaaggcggcgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCg              <  2:560606‑M2/114‑1 (MQ=255)
                                                                                                          gtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTGGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCCTTTACCATCCGTGATGGTCGTCGGTGTACTCTGCGTGCGCGTGAACGCGccgcttgtgc              >  2:702249‑M2/30‑139 (MQ=255)
                                                                                                           tgtcgtggaagacgacgacggcatgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGt             <  1:279957‑M2/115‑1 (MQ=255)
                                                                                                               tttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGGGAACTGCCCGCTTGTCGCCTGt         >  2:668712‑M2/25‑143 (MQ=255)
                                                                                                                   aagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTGGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCg     >  1:733065‑M2/21‑143 (MQ=255)
                                                                                                                      tcgacgacgctctgcatGTTCGTCTTCTTCTTTGTAGATGCGGCAGATCACGCCGGTAATTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTATTCTGTGTGTGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAt  <  2:550741‑M2/126‑1 (MQ=255)
                                                                                                                                       |                                                                                                                             
TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT  >  NC_000913/4001514‑4001774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.