BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp		insH21	insH21

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499	1300697	1199	123 [0]	[0] 123	insH21	insH21

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	108 (1.620)	65/90	0.0	100%	intergenic (+253/‑33)	ychE/insH21	putative inner membrane protein/IS5 transposase and trans‑activator
*	?	NC_000913	1300698 =	0 (0.000)	108 (1.620)	65/90	0.0	100%	intergenic (+151/‑484)	insH21/oppA	IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein

Only 100 of 123 total aligned reads displayed.

ATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAG  >  NC_000913/1299449‑1299547

atTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                                   <  1:2496883‑M1/50‑1 (MQ=255)

 ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                  >  1:3979485‑M1/1‑49 (MQ=255)

 ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                  >  1:4021512‑M1/1‑49 (MQ=255)

  tGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                  >  1:5249239‑M1/1‑48 (MQ=255)

  tGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                  >  1:3357022‑M1/1‑48 (MQ=255)

   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAat                                                 <  1:828271‑M1/49‑3 (MQ=255)

   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAat                                                 <  1:3554455‑M1/49‑3 (MQ=255)

    aaaTGATATTATTAATTTCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                <  1:2802713‑M1/49‑4 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                               <  1:4278828‑M1/50‑5 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                               >  1:5413874‑M1/1‑46 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                               <  1:339453‑M1/50‑5 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                               >  1:1189922‑M1/1‑46 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                               <  1:1179995‑M1/50‑5 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                <  1:3946606‑M1/49‑4 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                <  1:384344‑M1/49‑4 (MQ=255)

     aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataat                                              <  1:5506880‑M1/50‑6 (MQ=255)

     aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataat                                              <  1:442685‑M1/50‑6 (MQ=255)

      aTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                             <  1:1009705‑M1/50‑7 (MQ=255)

      aTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                             <  1:4856584‑M1/50‑7 (MQ=255)

      aTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataat                                              >  1:1298004‑M1/1‑44 (MQ=255)

       tGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                            >  1:482992‑M1/1‑43 (MQ=255)

       tGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                            >  1:4576348‑M1/1‑43 (MQ=255)

       tGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                            <  1:3762733‑M1/50‑8 (MQ=255)

        gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                           >  1:95345‑M1/1‑42 (MQ=255)

         atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                          <  1:137576‑M1/50‑10 (MQ=255)

         atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                          <  1:2030390‑M1/50‑10 (MQ=255)

         atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                           >  1:2509917‑M1/1‑41 (MQ=255)

         atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                           <  1:2265563‑M1/49‑9 (MQ=255)

         atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                           <  1:2746251‑M1/49‑9 (MQ=255)

           attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaa                                        >  1:4067277‑M1/1‑39 (MQ=255)

            ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                       >  1:849497‑M1/1‑38 (MQ=255)

             tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                      >  1:1708353‑M1/1‑37 (MQ=255)

             tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                      >  1:2641619‑M1/1‑37 (MQ=255)

              attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                      <  1:5084525‑M1/49‑14 (MQ=255)

               ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgt                                    >  1:582401‑M1/1‑35 (MQ=255)

               ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattg                                     <  1:29003‑M1/49‑15 (MQ=255)

                 aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                   >  1:1691332‑M1/1‑33 (MQ=255)

                 aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                   >  1:513144‑M1/1‑33 (MQ=255)

                 aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                   >  1:2600448‑M1/1‑33 (MQ=255)

                  aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                 <  1:2132734‑M1/50‑19 (MQ=255)

                  aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                  <  1:1627213‑M1/49‑18 (MQ=255)

                  aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                  >  1:5218178‑M1/1‑32 (MQ=255)

                  aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                  >  1:259358‑M1/1‑32 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                >  1:2903514‑M1/1‑31 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                <  1:1514296‑M1/50‑20 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                >  1:1076243‑M1/1‑31 (MQ=255)

                    tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               >  1:481443‑M1/1‑30 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               <  1:1672990‑M1/49‑21 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               >  1:2238645‑M1/1‑29 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               <  1:3687309‑M1/49‑21 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               <  1:3789475‑M1/49‑21 (MQ=255)

                      cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                             <  1:4775600‑M1/50‑23 (MQ=255)

                      cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                             <  1:4720525‑M1/50‑23 (MQ=255)

                      cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatt                              <  1:3308955‑M1/49‑22 (MQ=255)

                       aCTGCCTTTGGTAGAGGAAAGTGCTAAatactaatcaattgttaaattat                            <  1:2009898‑M1/50‑24 (MQ=255)

                       aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                            >  1:4274585‑M1/1‑27 (MQ=255)

                       aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                            >  1:1083241‑M1/1‑27 (MQ=255)

                          gCCTTTGGTAGAGGAGAGTGCTAAataataatcaattgttaaattattgt                         >  1:456101‑M1/1‑24 (MQ=255)

                          gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                         >  1:3664475‑M1/1‑24 (MQ=255)

                          gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                         >  1:5145346‑M1/1‑24 (MQ=255)

                          gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                         >  1:4708446‑M1/1‑24 (MQ=255)

                           ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                        <  1:4522734‑M1/50‑28 (MQ=255)

                           ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                         <  1:298645‑M1/49‑27 (MQ=255)

                           ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                         <  1:4946709‑M1/49‑27 (MQ=255)

                           ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattg                          <  1:2152757‑M1/48‑26 (MQ=255)

                            cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                        >  1:209190‑M1/1‑22 (MQ=255)

                            cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                        >  1:5367945‑M1/1‑22 (MQ=255)

                             tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                      >  1:1836655‑M1/1‑21 (MQ=255)

                               tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatt                    >  1:101619‑M1/1‑19 (MQ=255)

                               tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatt                    >  1:5280919‑M1/1‑19 (MQ=255)

                                ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatt                    <  1:1861546‑M1/49‑32 (MQ=255)

                                 gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttc                  <  1:3009803‑M1/50‑34 (MQ=255)

                                 gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                   <  1:4733839‑M1/49‑33 (MQ=255)

                                 gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                   <  1:5000504‑M1/49‑33 (MQ=255)

                                   agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcac                >  1:5535455‑M1/1‑15 (MQ=255)

                                    gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcact               <  1:5186679‑M1/50‑37 (MQ=255)

                                    gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcac                >  1:1600671‑M1/1‑14 (MQ=255)

                                    gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcac                >  1:2402513‑M1/1‑14 (MQ=255)

                                     agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta              <  1:1057265‑M1/50‑38 (MQ=255)

                                     agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta              >  1:507203‑M1/1‑13 (MQ=255)

                                      ggAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac             >  1:319707‑M1/1‑12 (MQ=255)

                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact            >  1:4006761‑M1/1‑11 (MQ=255)

                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact            <  1:324802‑M1/50‑40 (MQ=255)

                                        aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactg           >  1:4394816‑M1/1‑10 (MQ=255)

                                         aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactg           >  1:5369077‑M1/1‑9 (MQ=255)

                                           gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa        >  1:4612706‑M1/1‑7 (MQ=255)

                                            tGCTAAataataatcaattgttaaattattgtgcatttcactactggaac       >  1:3612001‑M1/1‑6 (MQ=255)

                                            tGCTAAataataatcaattgttaaattattgtgcatttcactactggaac       <  1:781604‑M1/50‑45 (MQ=255)

                                             gCTAAataataatcaattgttaaattattgtgcatttcactactggaact      >  1:5295357‑M1/1‑5 (MQ=255)

                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaact      <  1:3184781‑M1/49‑46 (MQ=255)

                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaact      <  1:307504‑M1/49‑46 (MQ=255)

                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaact      <  1:3633089‑M1/49‑46 (MQ=255)

                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactg     >  1:247058‑M1/1‑3 (MQ=255)

                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactg     >  1:3286049‑M1/1‑3 (MQ=255)

                                                aaataataatcaattgttaaattattgtgcatttcactactggaactgt    >  1:3260041‑M1/1‑2 (MQ=255)

                                                aaataataatcaattgttaaattattgtgcatttcactactggaactgt    >  1:5574557‑M1/1‑2 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgtaa  >  1:5290008‑M1/1‑1 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgta   <  1:3768600‑M1/49‑49 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgt    <  1:2537983‑M1/48‑48 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgt    <  1:2371805‑M1/48‑48 (MQ=255)

ATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAG  >  NC_000913/1299449‑1299547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`