Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,798,370	(TTC)4→3	coding (124‑126/405 nt)	stpA ←	DNA‑binding transcriptional repressor StpA with RNA chaperone activity

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2798361	2798363	3	19 [0]	[0] 22	stpA	DNA‑binding transcriptional repressor StpA with RNA chaperone activity

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2798364 =	0 (0.000)	16 (0.720)	14/80	0.3	100%	coding (132/405 nt)	stpA	DNA‑binding transcriptional repressor StpA with RNA chaperone activity
	?	NC_000913	= 2798360	0 (0.000)					coding (136/405 nt)	stpA	DNA‑binding transcriptional repressor StpA with RNA chaperone activity

GGTGCTAATTTTTTCCTGGCGCTCTGCCAGTTCACGCTGCTGCTGTTCTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGCATTT  >  NC_000913/2798316‑2798413                                                 |                                                  ccaggtgctaattttttcctggcgctctgccagttcacgctgctgctgtt                                                  <  1:176264‑M1/2‑1 (MQ=255)  caggtgctaattttttcctggcgctctgccagttcacgctgctgctgttc                                                 <  1:1034522‑M1/3‑1 (MQ=255)      tgctaattttttcctggcgctctgccagttcacgctgctgctgttcttct                                             <  1:1703177‑M1/7‑1 (MQ=255)           attttttcctggcgctctgccagttcacgctgctgctgTTCTTCTTCACg                                        <  1:246196‑M1/12‑1 (MQ=255)            ttttttcctggcgctctgccagttcacgctgctgctgTTCTTCTTCACGt                                       <  1:399790‑M1/13‑1 (MQ=255)                              ccagttcacgctgctgctgTTCTTCTTCACGTCTTTCTTTAGTGACa                        >  1:503920‑M1/20‑47 (MQ=255)                                agttcacgctgctgctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCt                   >  1:2190422‑M1/18‑50 (MQ=255)                                    cacgctgctgctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAAt               <  1:1283621‑M1/37‑1 (MQ=255)                                     acgctgctgctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAAt               >  1:1991102‑M1/13‑49 (MQ=255)                                      cgctgctgctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAAttt             >  1:2103655‑M1/12‑50 (MQ=255)                                      cgctgctgctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAAttt             >  1:188298‑M1/12‑50 (MQ=255)                                       gctgctgctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAAtttt            <  1:1956602‑M1/40‑1 (MQ=255)                                         tgctgctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTc          <  1:67446‑M1/42‑1 (MQ=255)                                            tgctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAg       <  1:449454‑M1/45‑1 (MQ=255)                                              ctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAg       >  1:1981539‑M1/4‑48 (MQ=255)                                              ctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGc      >  1:468604‑M1/4‑49 (MQ=255)                                              ctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGc      >  1:1326953‑M1/4‑49 (MQ=255)                                              ctgTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGCa     >  1:1839663‑M1/4‑50 (MQ=255)                                                gTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGCAtt   <  1:2121259‑M1/49‑1 (MQ=255)                                                 TTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGCAttt  <  1:2069902‑M1/50‑1 (MQ=255)                                                 TTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGCAttt  <  1:684853‑M1/50‑1 (MQ=255)                                                 TTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGCAttt  >  1:837704‑M1/1‑50 (MQ=255)                                                 |                                                  GGTGCTAATTTTTTCCTGGCGCTCTGCCAGTTCACGCTGCTGCTGTTCTTCTTCTTCACGTCTTTCTTTAGTGACAACCCTGAATTTTTCGAGCATTT  >  NC_000913/2798316‑2798413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.