Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	92,854	(GCG)4→3	coding (1442‑1444/1767 nt)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	92844	92846	3	25 [0]	[0] 25	ftsI	peptidoglycan DD‑transpeptidase FtsI

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	92847 =	0 (0.000)	23 (1.080)	18/76	0.1	100%	coding (1435/1767 nt)	ftsI	peptidoglycan DD‑transpeptidase FtsI
	?	NC_000913	= 92843	0 (0.000)					coding (1431/1767 nt)	ftsI	peptidoglycan DD‑transpeptidase FtsI

CGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAA  >  NC_000913/92805‑92895                                           |                                                 gtccgcactgtggtgcatatgatggaaagcgtggcgctaccaggcggc                                             >  1:1134229‑M1/43‑48 (MQ=255)      cactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTg                                       >  1:506200‑M1/38‑49 (MQ=255)      cactgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTg                                       >  1:1566601‑M1/38‑49 (MQ=255)       actgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTg                                       <  1:1823989‑M1/12‑1 (MQ=255)        ctgtggtgcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGaa                                     >  1:16194‑M1/36‑49 (MQ=255)               gcatatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAggcggc                               <  1:455091‑M1/20‑1 (MQ=255)                catatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGa                             <  1:835851‑M1/22‑1 (MQ=255)                 atatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGAt                            >  1:1412313‑M1/27‑49 (MQ=255)                  tatgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGAtt                           >  1:1267974‑M1/26‑49 (MQ=255)                    tgatggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTaa                         >  1:1177836‑M1/24‑49 (MQ=255)                      atggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTaaa                        >  1:1338820‑M1/22‑48 (MQ=255)                      atggaaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAg                       >  1:1591160‑M1/22‑49 (MQ=255)                          aaagcgtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCta                   >  1:1135301‑M1/18‑49 (MQ=255)                               gtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgta              <  1:684887‑M1/37‑1 (MQ=255)                               gtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgta              <  1:1741776‑M1/37‑1 (MQ=255)                               gtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgta              <  1:1114124‑M1/37‑1 (MQ=255)                               gtggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgta              <  1:1068097‑M1/37‑1 (MQ=255)                                tggcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtat             <  1:1447003‑M1/38‑1 (MQ=255)                                  gcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtatcg           >  1:891259‑M1/10‑49 (MQ=255)                                  gcgctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtatcg           >  1:1921122‑M1/10‑49 (MQ=255)                                    gctaccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGc          <  1:814020‑M1/41‑1 (MQ=255)                                       accaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCAt       <  1:1440173‑M1/44‑1 (MQ=255)                                        ccaGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTa     >  1:1902072‑M1/4‑49 (MQ=255)                                          aGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaa   >  1:1724717‑M1/2‑49 (MQ=255)                                           GGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaaa  <  1:1106525‑M1/49‑1 (MQ=255)                                           |                                                 CGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAA  >  NC_000913/92805‑92895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.