BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp		insH21	insH21

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499	1300697	1199	135 [0]	[0] 134	insH21	insH21

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	115 (1.150)	65/90	0.0	100%	intergenic (+253/‑33)	ychE/insH21	putative inner membrane protein/IS5 transposase and trans‑activator
*	?	NC_000913	1300698 =	0 (0.000)	115 (1.150)	65/90	0.0	100%	intergenic (+151/‑484)	insH21/oppA	IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein

Only 100 of 135 total aligned reads displayed.

ATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAG  >  NC_000913/1299449‑1299547

atTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                                   <  1:3951650‑M1/50‑1 (MQ=255)

atTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                                   <  1:1647253‑M1/50‑1 (MQ=255)

 ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                  <  1:2385973‑M1/50‑2 (MQ=255)

  tGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                  >  1:1095549‑M1/1‑48 (MQ=255)

   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAat                                                 <  1:7589041‑M1/49‑3 (MQ=255)

   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAat                                                 <  1:5190836‑M1/49‑3 (MQ=255)

   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAat                                                 <  1:2910851‑M1/49‑3 (MQ=255)

   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAat                                                 <  1:2541920‑M1/49‑3 (MQ=255)

   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAat                                                 >  1:1815786‑M1/1‑47 (MQ=255)

   gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAat                                                 <  1:7330190‑M1/49‑3 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                               >  1:5778160‑M1/1‑46 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                <  1:7102057‑M1/49‑4 (MQ=255)

    aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                <  1:4777122‑M1/49‑4 (MQ=255)

     aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataat                                              <  1:7961831‑M1/50‑6 (MQ=255)

       tGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataa                                            <  1:5534819‑M1/50‑8 (MQ=255)

        gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                           >  1:2413817‑M1/1‑42 (MQ=255)

         atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                          <  1:4266848‑M1/50‑10 (MQ=255)

         atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                           >  1:1975340‑M1/1‑41 (MQ=255)

          tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatca                                         <  1:4190363‑M1/50‑11 (MQ=255)

          tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatca                                         <  1:3863953‑M1/50‑11 (MQ=255)

          tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                          >  1:5669848‑M1/1‑40 (MQ=255)

            ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                       >  1:2463711‑M1/1‑38 (MQ=255)

            ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                       <  1:621458‑M1/50‑13 (MQ=255)

            ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                       >  1:3452779‑M1/1‑38 (MQ=255)

              attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattg                                     >  1:6538342‑M1/1‑36 (MQ=255)

                taATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgt                                    >  1:2690225‑M1/1‑34 (MQ=255)

                taATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgt                                    >  1:4938699‑M1/1‑34 (MQ=255)

                taATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgt                                    >  1:1753946‑M1/1‑34 (MQ=255)

                 aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                   >  1:1543346‑M1/1‑33 (MQ=255)

                 aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                   >  1:323238‑M1/1‑33 (MQ=255)

                 aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                   <  1:6211934‑M1/49‑17 (MQ=255)

                  aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                 <  1:523610‑M1/50‑19 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                >  1:615526‑M1/1‑31 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                >  1:2651981‑M1/1‑31 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                <  1:4576289‑M1/50‑20 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                <  1:4403563‑M1/50‑20 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                >  1:1689366‑M1/1‑31 (MQ=255)

                   ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                <  1:1414784‑M1/50‑20 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               <  1:5677833‑M1/49‑21 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               >  1:5056279‑M1/1‑29 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               >  1:5013538‑M1/1‑29 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               >  1:855119‑M1/1‑29 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               <  1:1328831‑M1/49‑21 (MQ=255)

                     ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                               <  1:3663288‑M1/49‑21 (MQ=255)

                      cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                             >  1:3706671‑M1/1‑28 (MQ=255)

                      cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatt                              <  1:801383‑M1/49‑22 (MQ=255)

                      cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatt                              <  1:2235358‑M1/49‑22 (MQ=255)

                       aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                            <  1:3304366‑M1/50‑24 (MQ=255)

                        cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                           <  1:4111270‑M1/50‑25 (MQ=255)

                        cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                           >  1:6413385‑M1/1‑26 (MQ=255)

                        cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                           >  1:3913070‑M1/1‑26 (MQ=255)

                        cTGCCTTTGGTAAAGGAAAGTGCTAAataataatcaattgttaaattatt                           >  1:5898415‑M1/1‑26 (MQ=255)

                          gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                         >  1:4825219‑M1/1‑24 (MQ=255)

                           ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                        <  1:3055405‑M1/50‑28 (MQ=255)

                           ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                         <  1:2912009‑M1/49‑27 (MQ=255)

                           ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                         <  1:6717599‑M1/49‑27 (MQ=255)

                            cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgc                       <  1:3854417‑M1/50‑29 (MQ=255)

                            cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                        >  1:6430849‑M1/1‑22 (MQ=255)

                             tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                      >  1:761275‑M1/1‑21 (MQ=255)

                              ttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcat                     >  1:833263‑M1/1‑20 (MQ=255)

                               tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatt                    >  1:636005‑M1/1‑19 (MQ=255)

                               tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatt                    >  1:4203475‑M1/1‑19 (MQ=255)

                               tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcat                     <  1:1530518‑M1/49‑31 (MQ=255)

                                ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                   >  1:1656020‑M1/1‑18 (MQ=255)

                                ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                   >  1:4912235‑M1/1‑18 (MQ=255)

                                 gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                   >  1:538656‑M1/1‑17 (MQ=255)

                                 gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                   <  1:7734748‑M1/49‑33 (MQ=255)

                                    gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcact               <  1:5939171‑M1/50‑37 (MQ=255)

                                    gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcact               <  1:3705807‑M1/50‑37 (MQ=255)

                                    gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcac                <  1:7088889‑M1/49‑36 (MQ=255)

                                     agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta              <  1:3559186‑M1/50‑38 (MQ=255)

                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact            <  1:7315239‑M1/50‑40 (MQ=255)

                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact            <  1:5528599‑M1/50‑40 (MQ=255)

                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact            >  1:2714804‑M1/1‑11 (MQ=255)

                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact            <  1:5041996‑M1/50‑40 (MQ=255)

                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact            <  1:4291300‑M1/50‑40 (MQ=255)

                                        aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactg           <  1:6440165‑M1/50‑41 (MQ=255)

                                        aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactg           >  1:7273735‑M1/1‑10 (MQ=255)

                                         aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg          <  1:1846691‑M1/50‑42 (MQ=255)

                                          aGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg          >  1:2528310‑M1/1‑8 (MQ=255)

                                           gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa        >  1:8177617‑M1/1‑7 (MQ=255)

                                           gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa        <  1:5490186‑M1/50‑44 (MQ=255)

                                            tGCTAAataataatcaattgttaaattattgtgcatttcactactggaac       >  1:2803734‑M1/1‑6 (MQ=255)

                                            tGCTAAataataatcaattgttaaattattgtgcatttcactactggaac       <  1:5154340‑M1/50‑45 (MQ=255)

                                             gCTAAataataatcaattgttaaattattgtgcatttcactactggaact      >  1:4766013‑M1/1‑5 (MQ=255)

                                             gCTAAataataatcaattgttaaattattgtgcatttcactactggaact      >  1:5645357‑M1/1‑5 (MQ=255)

                                             gCTAAataataatcaattgttaaattattgtgcatttcactactggaact      >  1:6551394‑M1/1‑5 (MQ=255)

                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaactg     >  1:356867‑M1/1‑4 (MQ=255)

                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaactg     >  1:3834239‑M1/1‑4 (MQ=255)

                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaactg     <  1:4800932‑M1/50‑47 (MQ=255)

                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaactg     >  1:3646583‑M1/1‑4 (MQ=255)

                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaactg     >  1:3303194‑M1/1‑4 (MQ=255)

                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactg     >  1:2174701‑M1/1‑3 (MQ=255)

                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactg     >  1:3197389‑M1/1‑3 (MQ=255)

                                                aaataataatcaattgttaaattattgtgcatttcactactggaactgt    >  1:4321151‑M1/1‑2 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgtaa  >  1:7064243‑M1/1‑1 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgtaa  <  1:5411193‑M1/50‑50 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgta   <  1:1235182‑M1/49‑49 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgta   <  1:6255055‑M1/49‑49 (MQ=255)

                                                 aataataatcaattgttaaattattgtgcatttcactactggaactgt    <  1:5332474‑M1/48‑48 (MQ=255)

ATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAG  >  NC_000913/1299449‑1299547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`