breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsGlu_KHP_A6_F406_I2_R1_S27_L008_R1_001.good.fq4,576,912457,691,200100.0%100.0 bases100 bases95.4%
errorsGlu_KHP_A6_F406_I2_R1_S27_L008_R2_001.good.fq4,492,492449,249,200100.0%100.0 bases100 bases92.2%
total9,069,404906,940,400100.0%100.0 bases100 bases93.8%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,652182.22.6100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000100000
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 50001648
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.075

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.48209

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input10:14:26 23 May 201910:15:59 23 May 20191 minute 33 seconds
Read alignment to reference genome10:15:59 23 May 201910:31:58 23 May 201915 minutes 59 seconds
Preprocessing alignments for candidate junction identification10:31:58 23 May 201910:33:48 23 May 20191 minute 50 seconds
Preliminary analysis of coverage distribution10:33:48 23 May 201910:37:22 23 May 20193 minutes 34 seconds
Identifying junction candidates10:37:22 23 May 201910:48:45 23 May 201911 minutes 23 seconds
Re-alignment to junction candidates10:48:45 23 May 201910:54:42 23 May 20195 minutes 57 seconds
Resolving best read alignments10:54:42 23 May 201910:57:33 23 May 20192 minutes 51 seconds
Creating BAM files10:57:33 23 May 201911:00:45 23 May 20193 minutes 12 seconds
Tabulating error counts11:00:45 23 May 201911:02:01 23 May 20191 minute 16 seconds
Re-calibrating base error rates11:02:01 23 May 201911:02:02 23 May 20191 second
Examining read alignment evidence11:02:02 23 May 201911:14:58 23 May 201912 minutes 56 seconds
Polymorphism statistics11:14:58 23 May 201911:15:01 23 May 20193 seconds
Output11:15:01 23 May 201911:16:06 23 May 20191 minute 5 seconds
Total 1 hour 1 minute 40 seconds