New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 23256457 (1.170)4 (0.090) 4/270 6.2 7.3% intergenic (+15/+33) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
?NC_000913 = 232571 50 (1.120)intergenic (+22/+26) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCGTACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/232407‑232564
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaaaaggcaccgATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGAATAGCGTCTACTAA  <  NC_000913/232571‑232424
                                                                                                                                                                                                                                                                                                                  
TGCGTACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAA                                                                                                                                                               <  1:120673/149‑1
TGCGTACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAA                                                                                                                                                               >  1:286574/1‑149
  CGTACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAA                                                                                                                                                             <  1:705835/149‑1
    TACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACATAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGG                                                                                                                                                           >  1:435448/1‑149
    TACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGG                                                                                                                                                           >  1:604317/1‑149
                                  CATTTACCAACAGAGCGCATCGACAGAGGTGAGAACATATTGTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGATTCCCCCGGTGCCTTTTTTATTTA                                                                                                                             <  1:247349/149‑1
                                                  GCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCA                                                                                                             <  2:544894/149‑1
                                                              GTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCATCTACCATGATG                                                                                                 <  1:432685/149‑1
                                                                                                                                               aaAAAAAAGGCACCGATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGA                <  2:289660/147‑1
                                                                                                                                                  AAAAAGGCACCGATTCCCCCGTGGCCTTTTTTATTTATGCCGCTTTATGCGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGAATA             <  2:109945/149‑1
                                                                                                                                                  AAAAAGGCACCGATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGAATA             >  1:140019/1‑149
                                                                                                                                                  AAAAAGGCACCGATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTAGATGCGCTCTGTTGGTCAATGCGAATA             >  1:78289/1‑149
                                                                                                                                                    AAAGGCACCGATTCCCCCGGGGCCTTTTTTATTTAGGCCGCTTTATGTGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGAATAGC           <  2:243527/149‑1
                                                                                                                                                       GGCACCGATTCCCCCGGTGCCTTTTTTGTTTATGCCGCTTTATGTGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGACAATTACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGAATAGCGTC        <  1:364822/149‑1
                                                                                                                                                        GCACCGATTCCCCCGGTTTCTTTTTTATTTATGAAAGATTATGTTCTTTTACCATGTTGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGAATAGCGTCT       >  2:178168/1‑149
                                                                                                                                                             GATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGAATAGCGTCTACTAA  >  1:646877/1‑149
                                                                                                                                                                                                                                                                                                                  
TGCGTACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/232407‑232564
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaaaaggcaccgATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCATCTACCATGATGATATCACTGGTGAAAAAGCCATCATTCTGCAAGGCAAAATACGTTCTCACCTCTGTCGATGCGCTCTGTTGGTAAATGCGAATAGCGTCTACTAA  <  NC_000913/232571‑232424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.