New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 232564	57 (1.170)	4 (0.090)	4/270	6.2	7.3%	intergenic (+15/+33)	yafE/mltD	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
	?	NC_000913	= 232571	50 (1.120)					intergenic (+22/+26)	yafE/mltD	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGT  >  NC_000913/232424‑232711                                                                                                                                                    |                                                                                                                                             ttAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATc                                                                                                                                             <  1:646877/149‑1 (MQ=255)      agACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCAACATGGTAAAAGAACATAATCTTTCATAAATAAAAAAGAAACCGGGGGAATCGGTGc                                                                                                                                        <  2:178168/149‑1 (MQ=255)       gACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTAATTGTCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAACAAAAAAGGCACCGGGGGAATCGGTGcc                                                                                                                                       >  1:364822/1‑149 (MQ=255)          gCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCCTAAATAAAAAAGGCCCCGGGGGAATCGGTGCCttt                                                                                                                                    >  2:243527/1‑149 (MQ=255)            tATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCGCATAAAGCGGCATAAATAAAAAAGGCCACGGGGGAATCGGTGCCttttt                                                                                                                                  >  2:109945/1‑149 (MQ=255)            tATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCttttt                                                                                                                                  <  1:140019/149‑1 (MQ=255)            tATTCGCATTGACCAACAGAGCGCATCTACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCttttt                                                                                                                                  <  1:78289/149‑1 (MQ=255)               tCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCtttttttt                                                                                                                               >  2:289660/1‑149 (MQ=255)                   aTTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCt                                                                                                                           >  1:489367/1‑149 (MQ=255)                       aCCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGtt                                                                                                                       >  1:562404/1‑149 (MQ=255)                         cAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGGGCCTTTTTATTATCTGGTTTg                                                                                                                     >  1:323453/1‑149 (MQ=255)                          aaCAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGt                                                                                                                    <  2:715427/149‑1 (MQ=255)                          aaCAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGt                                                                                                                    <  1:292566/149‑1 (MQ=255)                             cgagCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCTGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAACGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAg                                                                                                                 <  2:93046/148‑1 (MQ=255)                             agagCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGGGCCTTTTTATTATCTGGTTTGTCAg                                                                                                                 >  1:377973/1‑149 (MQ=255)                                 cgcATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAAt                                                                                                             >  2:182464/1‑149 (MQ=255)                                    aTCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCGCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          >  2:41970/1‑149 (MQ=255)                                    aTCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTGTATTATCTGGTTTCTCAGGACTCTg                                                                                                          >  2:197260/1‑149 (MQ=255)                                              gTGAGAACGGATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCCCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgtt                                                                                                <  1:465456/149‑1 (MQ=255)                                                    aCGTATTTTGCCGTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAACTAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGttttt                                                                                          <  1:125239/149‑1 (MQ=255)                                                     cGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCATTGATAGCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGCATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAGTCTGGCATGTTGTTGTTTTTc                                                                                         <  1:578591/149‑1 (MQ=255)                                                         ttttGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaa                                                                                     >  1:382036/1‑149 (MQ=255)                                                          tttGCCTTGCAGAATGATGGCACTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaaa                                                                                    <  1:106096/149‑1 (MQ=255)                                                             gCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaaacaa                                                                                 >  1:584124/1‑149 (MQ=255)                                                                    aGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAACCAACGTCAGc                                                                          >  2:534536/1‑149 (MQ=255)                                                                     gAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAATCAACGTCAGCt                                                                         >  1:311021/1‑149 (MQ=255)                                                                              cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTg                                                                >  1:763010/1‑149 (MQ=255)                                                                              cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTg                                                                <  2:94118/149‑1 (MQ=255)                                                                               tttttCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTgg                                                               >  1:334970/1‑149 (MQ=255)                                                                                 tttCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGtt                                                             <  2:284924/149‑1 (MQ=255)                                                                                 tttCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGGGCCGTTTTATTATATGGGTTGTCAGGAATCTGGCATGGTGTTGTTTTTCACAACAAACGTCAGCTCATCGCCTGGtt                                                             >  2:230791/1‑149 (MQ=255)                                                                                     aCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCCGCTTATCGCCTGGTTGCAg                                                         >  1:337879/1‑149 (MQ=255)                                                                                         gTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTGAGCTTATCGCCTGGTTGCAGATTc                                                     <  1:415469/149‑1 (MQ=255)                                                                                         gTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTc                                                     <  1:41970/149‑1 (MQ=255)                                                                                           gATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGc                                                   <  1:594362/149‑1 (MQ=255)                                                                                            atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGCATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTGGTTGTTTTTCAAAAACAACGTCAGCTTATCGCCTGGTTGGAGATTCgac                                                  >  2:2418/1‑147 (MQ=255)                                                                                              atcagcATGGTAGCTGCATATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgt                                                <  1:109945/149‑1 (MQ=255)                                                                                               tcatcaTGGTAGATGTACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgtg                                               <  1:35517/149‑1 (MQ=255)                                                                                                catcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgtgt                                              >  1:170619/1‑149 (MQ=255)                                                                                                         aGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTATTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATGGCCTGATTGCAGATTCGCAGGGCAGCTTTTcc                                     >  2:669752/1‑149 (MQ=255)                                                                                                          gATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCa                                    <  2:334970/149‑1 (MQ=255)                                                                                                            tGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTGTTGCACAAACACAGTCGTCTTATCGCCTGGTTGCAGATTCGCAGTTTCGCTGTTCCAgc                                  <  2:377973/149‑1 (MQ=255)                                                                                                                 aTAAAGCGGCAGAAATAAAAAAGGCGCCGGGGGGATCGGTGCCTTTTTATTATCGGGTTTGTCATGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCacc                             <  2:648283/149‑3 (MQ=255)                                                                                                                   aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATcac                           >  2:774679/1‑149 (MQ=255)                                                                                                                       cGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCt                       <  2:584124/149‑1 (MQ=255)                                                                                                                        ggaataaataaAATAGGCACCTGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCtt                      <  1:154771/146‑1 (MQ=255)                                                                                                                        ggCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCtt                      >  2:620932/1‑149 (MQ=255)                                                                                                                           ataaataaAAAAGGCACCGGGGTGATCGGTGTCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGa                   <  2:383488/149‑1 (MQ=255)                                                                                                                           ataaataaAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGa                   >  1:749058/1‑149 (MQ=255)                                                                                                                           ataaataaAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACAACTTTGa                   >  1:775124/1‑149 (MQ=255)                                                                                                                            taaataaaAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGGTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATGCGCATGTTCGCTTTGCCAGCGCATCACATCTTTGAt                  <  2:382036/149‑1 (MQ=255)                                                                                                                                       ggCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGt       <  2:489367/149‑1 (MQ=255)                                                                                                                                         cACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTATTGTTTTTCACAAACAACGTCAGCTTATCGCCTTGTTGAAGATTCCCAGTGTCGCTGTTCCAGCGCATCACATCTTTTTATTTCACGCCGTgg     >  1:110573/1‑149 (MQ=255)                                                                                                                                          aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGttttt                                                                                          <  2:164977/63‑1 (MQ=255)                                                                                                                                          aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGttttt                                                                                          >  1:164977/1‑63 (MQ=255)                                                                                                                                          aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGc    >  2:670823/1‑149 (MQ=255)                                                                                                                                          aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGc    <  1:522689/149‑1 (MQ=255)                                                                                                                                            cGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCgg  >  2:35698/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                             TTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGT  >  NC_000913/232424‑232711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.