| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 4,185,992 | Δ18 bp | coding (644‑661/4224 nt) | rpoC → | RNA polymerase, beta prime subunit |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 4185992 | 4186009 | 18 | 62 [0] | [0] 62 | rpoC | RNA polymerase, beta prime subunit |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 4185991 | 0 (0.000) | 56 (0.910) | 48/274 | 0.3 | 100% | coding (643/4224 nt) | rpoC | RNA polymerase, beta prime subunit |
| ? | NC_000913 | 4186010 = | 0 (0.000) | coding (662/4224 nt) | rpoC | RNA polymerase, beta prime subunit | |||||
TTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATC > NC_000913/4185862‑4186156 | ctggacgcgctggaagagttcggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaacccactccgaaaccaagcgtat > 2:111113‑M2/149‑149 (MQ=255)ctggacgcgctggaagagttcggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtat > 2:110924‑M2/149‑149 (MQ=255) gctggaagagttcggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAActgc > 1:154507‑M2/141‑149 (MQ=255) ctggaagagttcggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggaggaagagtgcgaacagctgcgtgaagagctgaacgaaacctactccgaaaccaagcgtaTCAAActgct < 1:659170‑M2/10‑1 (MQ=255) ctggaagagttcggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAActgct < 1:260603‑M2/10‑1 (MQ=255) aagagttcggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGaa < 2:163729‑M2/14‑1 (MQ=255) gttcggtgacgaattcgacgcgaagatgggggcggaagcaatccgggctctacggaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGcgt < 1:478400‑M2/18‑1 (MQ=255) ttcggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACAGCTGGAAGcgtt > 2:88430‑M2/131‑149 (MQ=255) ggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGcgttcgt > 1:691659‑M2/128‑149 (MQ=255) ggtgacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGcgttcgt > 1:691553‑M2/128‑149 (MQ=255) gacgaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCa > 1:982247‑M2/125‑149 (MQ=255) gacgaattcgacgcgaagagggggtcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCa < 2:115131‑M2/25‑1 (MQ=255) gaattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaCCAAACTGCTGGAAGCGCACGTTCAGTc > 2:759084‑M2/122‑149 (MQ=255) aattcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCt > 1:27527‑M2/121‑149 (MQ=255) tcgacgcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGt > 1:860264‑M2/118‑149 (MQ=255) gcgaagatgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCCAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACaa > 2:1054560‑M2/113‑149 (MQ=255) gaagatgggggcggaagcaatccaggctctgctgaagagcatggataaggtgcaagattgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGCAAcaaaa > 2:822773‑M2/111‑148 (MQ=255) atgggggcgggagcaatccaggctctggtgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCaga < 1:64538‑M2/43‑1 (MQ=255) tgggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCagag < 2:365060‑M2/44‑1 (MQ=255) ggggcggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTg > 2:674878‑M2/104‑149 (MQ=255) ggaagcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGCAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGGGgatga > 2:850950‑M2/99‑149 (MQ=255) gcaatccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctacgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTGTGGTAACAAACCAGAGTGGATGATCCt > 1:622637‑M2/95‑149 (MQ=255) atccaggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagacctgaaagaaaccaactccgaaaccaagcgtaTCAAACAGGTGGAAGCGTACGTTCAGTCTGGTAACAAACCAGAGTGTAGGATCCTGAc > 1:908434‑M2/92‑149 (MQ=255) caggctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGGGGATGATCCTGACCGt > 1:438469‑M2/89‑149 (MQ=255) caggctctgctgaagagcatggatctggagcaacggccctaacagctgcgtgaagagctgaacgaacccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATGCTGACCGt > 2:775605‑M2/89‑149 (MQ=255) ctctgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACAAGAGTGGATGATCCTGACCGTCCTg > 1:1089468‑M2/85‑149 (MQ=255) tgctgaagagcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCg > 1:822656‑M2/82‑149 (MQ=255) agcatggatctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGATCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgcc > 1:486948‑M2/74‑149 (MQ=255) caggggtctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCGGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTgccgc < 1:310550‑M2/78‑1 (MQ=255) atctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGAt < 2:154507‑M2/83‑1 (MQ=255) tctggagcaagagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATc < 1:676983‑M2/84‑1 (MQ=255) ctggagcaagagtgcgaacagcggcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCt < 2:509904‑M2/85‑1 (MQ=255) agagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCCGATCTGCGTCCGc > 2:513660‑M2/57‑149 (MQ=255) gagtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGGGGATGATCCTGACCGTTCTGCCGGTCCTGCCGCCAGATCTGCGTCCGCt > 2:354286‑M2/56‑149 (MQ=255) agtgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTg < 2:446245‑M2/95‑1 (MQ=255) tgcgaacagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGt > 2:535017‑M2/53‑149 (MQ=255) acagctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaACAAACTGCTGGAAGCGTTCGTTCAGGCTGGTAACAAACAGGAGTGGATGATCCTGACCGTTCTGCCGGTGCTGCCGACAGATCTGCGTCCGATGGTTCGGc > 1:293299‑M2/48‑149 (MQ=255) cagctgcgtgaagggctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCt < 1:361473‑M2/103‑1 (MQ=255) agctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTg > 2:1061961‑M2/46‑149 (MQ=255) gctgcgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTgg < 1:494358‑M2/105‑1 (MQ=255) cgtgaagagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGAtgg > 1:126000‑M2/41‑149 (MQ=255) aagagctgaacgaaaccaaccccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATggtggt < 2:357439‑M2/113‑1 (MQ=255) agagctgaacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTc > 2:2201‑M2/36‑149 (MQ=255) aacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCCGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTcgc > 1:960973‑M2/29‑149 (MQ=255) aacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTcgc < 2:407582‑M2/121‑1 (MQ=255) aacgaaaccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGCTCGTTTcgc > 2:430251‑M2/29‑149 (MQ=255) accaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGGGGATGATCCTGACCGTTCTGCCGGGACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGCGGTCGTTTCGCGACTTc > 2:115178‑M2/23‑149 (MQ=255) accaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCACAGTGGATGATCCTGACAGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTTGTTTCGCGACGTc > 1:909266‑M2/23‑149 (MQ=255) ccaactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGCTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCt > 1:774484‑M2/22‑149 (MQ=255) caactccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGGAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTg > 2:181211‑M2/21‑149 (MQ=255) actccgaaaccaagcgcaTAAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGCGTGGATGATCCTGACCGTTCGGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGAc < 1:288353‑M2/131‑1 (MQ=255) tccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCt > 2:486668‑M2/17‑149 (MQ=255) tccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCt > 1:161949‑M2/17‑149 (MQ=255) ccgaaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTg > 2:819132‑M2/16‑149 (MQ=255) aaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCTAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAAc < 2:691659‑M2/137‑1 (MQ=255) aaaccaagcgtaTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAAc < 2:691553‑M2/137‑1 (MQ=255) caagcgtaGCAACCTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGATGGGATGAGCCTGACCGTTCTGCCGGTACTGCCGCCAGAGCTTCGTCCGCTGGTTCCGCTGGCTGGTGGTCGTTTCGCGACTTCTGACCTGAACGATc < 2:656569‑M2/141‑1 (MQ=255) agcgtaTCAAACCGCCGGAAGCGTTCGTCCAGTCTGGTAACAAACCAGAGTGGATGATCCCGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTg < 2:581606‑M2/143‑1 (MQ=255) gtttCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATTATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTAt < 2:504103/146‑1 (MQ=255) taTCAAACTGCTGGCAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACGGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATc < 1:238583‑M2/147‑1 (MQ=255) taTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATc < 2:822656‑M2/147‑1 (MQ=255) taTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATc < 1:454551‑M2/147‑1 (MQ=255) | TTCGGTGACGAATTCGACGCGAAGATGGGGGCGGAAGCAATCCAGGCTCTGCTGAAGAGCATGGATCTGGAGCAAGAGTGCGAACAGCTGCGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATC > NC_000913/4185862‑4186156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |