New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3210131 =76 (0.790)3 (0.030) 3/244 NT 3.8% coding (413/1014 nt) tsaD tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein
?NC_000913 3210172 = 81 (0.900)coding (372/1014 nt) tsaD tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein
Rejected: Frequency below/above cutoff threshold.

GCGCGCTACTGGTTGGCGCGACCGTGGGGCGTTCTCTGGCGTTTGCCTGGGACGTTCCGGCGATCCCTGTACACCATATGGAAGGGCATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTCCGGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3210287‑3210131
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttccggcggGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACA  >  NC_000913/3210172‑3210319
                                                                                                                                                                                                                                                                                                                 
GCGCGCTACTGGTTGGCGCGACCGTGGGGCGTTCTCTGGCGTTTGCCTGGGACGTTCCGGCGATCCCTGTACACCATATGGAAGGGCATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTT                                                                                                                                                              <  2:189667/149‑1
GCGCGCTACTGGTTGGCGCGACCGTGGGGCGTTCTCTGGCGTTTGCCTGGGACGTTCCGGCGATCCCTGTACACCATATGGAAGGGCATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTT                                                                                                                                                              <  2:816654/149‑1
GCGCGCTACTGGTTGGCGCGACCGTGGGGCGTTCTCTGGCGTTTGCCTGGGACGTTCCGACGATCCCTGTACACCATATGGAAGGGCATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTGGTTGCGCTGCTTGTT                                                                                                                                                              >  4:854398/1‑149
  GCGCTACTGGTTGGCGCGACCGTGGGGCGTTCTCTGGCGTTTGCCTGGGACGTTCCGGCGATCCCTGTACACCATATGGAAGGGCATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTC                                                                                                                                                            <  3:425555/149‑1
       ACTGGTTGGCGCGACCATGGGGCGTTCTCTGGCGTTTGCCTGGGACGTTCCGGCGCTCCCTGTACACCATATGGAAGGGCATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTCCGGCG                                                                                                                                                       >  4:379312/1‑149
                                                                                       ATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTCCGGCG                                                                                                                                                       >  4:409493/1‑69
                                                                                       ATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTCCGGCG                                                                                                                                                       <  3:409493/69‑1
                                                                                                             AGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGC                                                 >  1:772122/1‑149
                                                                                                                      GCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATC                                                                                               >  2:199201/1‑94
                                                                                                                      GCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATC                                                                                               <  1:199201/94‑1
                                                                                                                                                    TTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTAT          >  2:90496/1‑149
                                                                                                                                                     TCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATA         <  3:701053/149‑1
                                                                                                                                                     TCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATA         <  1:426835/149‑1
                                                                                                                                                     TCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATA         <  4:532761/149‑1
                                                                                                                                                      CCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAG        <  2:27034/149‑1
                                                                                                                                                       CGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGG       <  1:749758/149‑1
                                                                                                                                                          CGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCA    <  1:320974/149‑1
                                                                                                                                                           GGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCAAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACAAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCAC   >  2:79275/1‑149
                                                                                                                                                            GGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACA  <  3:187320/149‑1
                                                                                                                                                            GGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACA  <  4:610708/149‑1
                                                                                                                                                                                                                                                                                                                 
GCGCGCTACTGGTTGGCGCGACCGTGGGGCGTTCTCTGGCGTTTGCCTGGGACGTTCCGGCGATCCCTGTACACCATATGGAAGGGCATCTGTTAGCGCCGATGCTGGAAGATAACCCGCCGGAATTTCCGTTTGTTGCGCTGCTTGTTTCCGGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3210287‑3210131
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttccggcggGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACA  >  NC_000913/3210172‑3210319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.