New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3124692 =	44 (0.680)	3 (0.050)	3/252	NT	6.2%	coding (768/1224 nt)	glcF	glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
	?	NC_000913	3124729 =	48 (0.760)					coding (731/1224 nt)	glcF	glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

GTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAG  >  NC_000913/3124545‑3124839                                                                                                                                                    |                                                                                                                                                    gTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCtt                                                                                                                                                    <  3:245506/149‑1 (MQ=255)  tGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCttt                                                                                                                                                   <  3:383470/149‑1 (MQ=255)  ctggggatcagcgtcatgccagctaacgaagcaggctgttgtggcgcggtggactatcatcttaatgcgcaggagaaagggctggcacgggcgcgcaataatattgatgcctggtggcccgcgattgaagcaggtgccgaggcaatttt                                                                                                                                                   >  2:25840‑M1/147‑149 (MQ=255)          cAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAAcg                                                                                                                                           >  1:408486/1‑149 (MQ=255)            gCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAAcgcg                                                                                                                                         >  3:527814/1‑149 (MQ=255)                           gAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCg                                                                                                                          <  1:35637/149‑1 (MQ=255)                             gaagcaggctgttgtggcgcggtggactatcatcttaatgcgcaggagaaagggctggcacgggcgcgcaataatattgatgcctggtggcccgcgattgaagcaggtgccgaggcaatTTTG‑CAAACGCGCCGCAGCCGCTGGCGGtt                                                                                                                       >  3:87340‑M1/120‑149 (MQ=255)                               tGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGttt                                                                                                                      <  4:527814/149‑1 (MQ=255)                               tGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGttt                                                                                                                      <  2:188455/149‑1 (MQ=255)                               tGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGttt                                                                                                                      <  3:254211/149‑1 (MQ=255)                               tGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGttt                                                                                                                      <  1:591680/149‑1 (MQ=255)                                     tttttCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCaaaa                                                                                                                <  1:537978/149‑1 (MQ=255)                                     tttttCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCaaaa                                                                                                                <  3:431932/149‑1 (MQ=255)                                         tCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGc                                                                                                            <  3:330759/149‑1 (MQ=255)                                           cAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCt                                                                                                          >  3:290217/1‑149 (MQ=255)                                                   ccTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCt                                                                                                  <  4:109824/149‑1 (MQ=255)                                                   ccTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCt                                                                                                  <  2:180419/149‑1 (MQ=255)                                                        tatcatcttaatgcgcaggagaaagggctggcacgggcgcgcaataatattgatgcctggtggcccgcgattgaagcaggtgccgaggcaatTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCaaa                                                                                                                 >  1:593475‑M1/93‑129 (MQ=255)                                                        tatcatcttaatgcgcaggagaaagggctggcacgggcgcgcaataatattgatgcctggtggcccgcgattgaagcaggtgccgaggcaatTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCaaa                                                                                                                 <  2:593475‑M1/37‑1 (MQ=255)                                                           aGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAAt                                                                                          >  1:482746/1‑149 (MQ=255)                                                               gTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATcgcg                                                                                      >  2:14016/1‑149 (MQ=255)                                                                   gACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGcc                                                                                  >  1:593236/1‑149 (MQ=255)                                                                     cTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGccac                                                                                >  2:150124/1‑149 (MQ=255)                                                                        aaTCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAg                                                                             <  1:150124/149‑1 (MQ=255)                                                                         aTCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAgg                                                                            >  4:128686/1‑149 (MQ=255)                                                                            gACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAACTTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCAt                                                                         <  4:290217/149‑1 (MQ=255)                                                                                gCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAat                                                                     >  4:98869/1‑149 (MQ=255)                                                                                 ccAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAata                                                                    <  1:165163/149‑1 (MQ=255)                                                                                              ctgacGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCttt                                                                                                                                                   <  3:453342/57‑1 (MQ=255)                                                                                              ctgacGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCttt                                                                                                                                                   >  4:453342/1‑57 (MQ=255)                                                                                                  cGTTCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGc                                                   <  4:288503/149‑1 (MQ=255)                                                                                                      cTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGc                                               >  2:411529/1‑149 (MQ=255)                                                                                                       tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGc                                                                                                      >  1:344899/1‑93 (MQ=255)                                                                                                       tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGc                                                                                                      <  2:344899/93‑1 (MQ=255)                                                                                                       tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatta                                                                 <  1:168199/130‑1 (MQ=255)                                                                                                       tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatta                                                                 >  2:168199/1‑130 (MQ=255)                                                                                                       tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGcc                                              >  1:186218/1‑149 (MQ=255)                                                                                                           ccGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCttt                                          >  2:230516/2‑149 (MQ=255)                                                                                                            cGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTtc                                         >  1:135119/1‑149 (MQ=255)                                                                                                            cGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTtc                                         <  3:128686/149‑1 (MQ=255)                                                                                                             ggCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTtct                                        >  4:582990/1‑149 (MQ=255)                                                                                                                atatAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatta                                                                 <  2:21096/121‑1 (MQ=255)                                                                                                                atatAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatta                                                                 >  1:21096/1‑121 (MQ=255)                                                                                                                 tataACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc                                                                           >  4:6237/1‑110 (MQ=255)                                                                                                                 tataACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc                                                                           <  3:6237/110‑1 (MQ=255)                                                                                                                  ataACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTgc                                   >  1:509955/1‑149 (MQ=255)                                                                                                                     aCGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTgcgga                                >  1:290898/1‑147 (MQ=255)                                                                                                                          tCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAg                           >  3:162191/1‑149 (MQ=255)                                                                                                                                ttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATa                     <  1:377142/149‑1 (MQ=255)                                                                                                                                ttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATa                     <  1:565616/149‑1 (MQ=255)                                                                                                                                ttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATa                     <  2:308958/149‑1 (MQ=255)                                                                                                                                  cAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGt                   >  2:295771/1‑149 (MQ=255)                                                                                                                                                aCTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCacaa     <  3:37728/149‑1 (MQ=255)                                                                                                                                                  tctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc                                                                           >  1:590341/1‑77 (MQ=255)                                                                                                                                                  tctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc                                                                           <  2:590341/77‑1 (MQ=255)                                                                                                                                                  tctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCacaaca   >  2:297683/1‑149 (MQ=255)                                                                                                                                                   ctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAg  >  3:559956/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAG  >  NC_000913/3124545‑3124839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.