New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3124692 =	44 (0.680)	3 (0.050)	3/252	NT	6.2%	coding (768/1224 nt)	glcF	glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
	?	NC_000913	3124729 =	48 (0.760)					coding (731/1224 nt)	glcF	glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

TTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAG  >  NC_000913/3124581‑3124874                                                                                                                                                     |                                                                                                                                                  tttttCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCaaaa                                                                                                                                                   <  3:431932/149‑1 (MQ=255) tttttCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCaaaa                                                                                                                                                   <  1:537978/149‑1 (MQ=255)     tCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGc                                                                                                                                               <  3:330759/149‑1 (MQ=255)       cAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCt                                                                                                                                             >  3:290217/1‑149 (MQ=255)               ccTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCt                                                                                                                                     <  2:180419/149‑1 (MQ=255)               ccTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCt                                                                                                                                     <  4:109824/149‑1 (MQ=255)                       aGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAAt                                                                                                                             >  1:482746/1‑149 (MQ=255)                           gTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATcgcg                                                                                                                         >  2:14016/1‑149 (MQ=255)                               gACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGcc                                                                                                                     >  1:593236/1‑149 (MQ=255)                                 cTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGccac                                                                                                                   >  2:150124/1‑149 (MQ=255)                                    aaTCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAg                                                                                                                <  1:150124/149‑1 (MQ=255)                                     aTCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAgg                                                                                                               >  4:128686/1‑149 (MQ=255)                                        gACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAACTTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCAt                                                                                                            <  4:290217/149‑1 (MQ=255)                                            gCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAat                                                                                                        >  4:98869/1‑149 (MQ=255)                                             ccAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAata                                                                                                       <  1:165163/149‑1 (MQ=255)                                                              cGTTCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGc                                                                                      <  4:288503/149‑1 (MQ=255)                                                                  cTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGc                                                                                  >  2:411529/1‑149 (MQ=255)                                                                   tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGc                                                                                                                                         <  2:344899/93‑1 (MQ=255)                                                                   tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGc                                                                                                                                         >  1:344899/1‑93 (MQ=255)                                                                   tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatta                                                                                                    <  1:168199/130‑1 (MQ=255)                                                                   tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatta                                                                                                    >  2:168199/1‑130 (MQ=255)                                                                   tttATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGcc                                                                                 >  1:186218/1‑149 (MQ=255)                                                                       ccGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCttt                                                                             >  2:230516/2‑149 (MQ=255)                                                                        cGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTtc                                                                            >  1:135119/1‑149 (MQ=255)                                                                        cGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTtc                                                                            <  3:128686/149‑1 (MQ=255)                                                                         ggCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTtct                                                                           >  4:582990/1‑149 (MQ=255)                                                                            atatAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatta                                                                                                    >  1:21096/1‑121 (MQ=255)                                                                            atatAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatta                                                                                                    <  2:21096/121‑1 (MQ=255)                                                                             tataACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc                                                                                                              >  4:6237/1‑110 (MQ=255)                                                                             tataACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc                                                                                                              <  3:6237/110‑1 (MQ=255)                                                                              ataACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTgc                                                                      >  1:509955/1‑149 (MQ=255)                                                                                 aCGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTgcgga                                                                   >  1:290898/1‑147 (MQ=255)                                                                                      tCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAg                                                              >  3:162191/1‑149 (MQ=255)                                                                                            ttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATa                                                        <  2:308958/149‑1 (MQ=255)                                                                                            ttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATa                                                        <  1:565616/149‑1 (MQ=255)                                                                                            ttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATa                                                        <  1:377142/149‑1 (MQ=255)                                                                                              cAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGt                                                      >  2:295771/1‑149 (MQ=255)                                                                                                            aCTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCacaa                                        <  3:37728/149‑1 (MQ=255)                                                                                                              tctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc                                                                                                              >  1:590341/1‑77 (MQ=255)                                                                                                              tctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc                                                                                                              <  2:590341/77‑1 (MQ=255)                                                                                                              tctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCacaaca                                      >  2:297683/1‑149 (MQ=255)                                                                                                               ctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAg                                     >  3:559956/1‑149 (MQ=255)                                                                                                                tttgcaaaccgccagcggctgcggcgcgtttgtcaaaATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATa                                                        <  2:593475‑M2/92‑1 (MQ=255)                                                                                                                tttgcaaaccgccagcggctgcggcgcgtttgtcaaaATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATa                                                        >  1:593475‑M2/38‑129 (MQ=255)                                                                                                                 TTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGcc                                   >  3:98869‑M1/1‑149 (MQ=255)                                                                                                                 TTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGcc                                   >  2:43990‑M1/1‑149 (MQ=255)                                                                                                                     cAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCt                               >  1:329374/1‑149 (MQ=255)                                                                                                                       aaccgccagcggctgcggcgcgtttgcaaaATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTc                             >  3:87340‑M2/31‑149 (MQ=255)                                                                                                                       aaCGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTc                             <  2:408486/149‑1 (MQ=255)                                                                                                                         cgcgCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGt                           <  4:443591/149‑1 (MQ=255)                                                                                                                              cgcAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCt                      <  3:84053/149‑1 (MQ=255)                                                                                                                                  gCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAgat                                                            >  3:2611/1‑107 (MQ=255)                                                                                                                                  gCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAgat                                                            <  4:2611/107‑1 (MQ=255)                                                                                                                                  gCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCa                  >  1:29486/1‑149 (MQ=255)                                                                                                                                     gCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATTGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGa               <  1:521942/149‑1 (MQ=255)                                                                                                                                        ggCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGc            <  1:14016/149‑1 (MQ=255)                                                                                                                                             tttGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATc       <  1:230516/149‑1 (MQ=255)                                                                                                                                                  aaaATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAg  >  2:25840‑M2/4‑149 (MQ=255)                                                                                                                                                     aTTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTc                             <  2:435149/119‑1 (MQ=255)                                                                                                                                                     aTTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTc                             >  1:435149/1‑119 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAG  >  NC_000913/3124581‑3124874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.