New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3219874	39 (0.600)	3 (0.050)	3/248	NT	6.7%	coding (381/1380 nt)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
	?	NC_000913	= 3219902	47 (0.760)					coding (409/1380 nt)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

ACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCAC  >  NC_000913/3219728‑3220020                                                                                                                                                      |                                                                                                                                                   acCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GAt                                                                                                                                                  <  4:498669/149‑1 (MQ=255)    cAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGtt                                                                                                                                               <  3:360634/149‑1 (MQ=255)           aGGAGTTTATCGACTGCCTGGGAGGGTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGaaa                                                                                                                                        >  4:545048/1‑149 (MQ=255)                tttATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCt                                                                                                                                   >  1:398347/1‑149 (MQ=255)                 ttATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCtt                                                                                                                                  <  4:16146/149‑1 (MQ=255)                    tCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAATCCTtgct                                                                                                                               >  3:534744/1‑149 (MQ=255)                            cTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGttagcgcagcaagggttttcgccaac                                                                                                                          <  3:469229‑M1/149‑27 (MQ=255)                              gggAGGTTTTGGGATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCATCTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAAcgc                                                                                                                     >  1:243514‑M2/1‑146 (MQ=255)                              gggAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑Gttagcgcagcaagggttttcgccaacatcgc                                                                                                                     >  2:349397‑M1/1‑118 (MQ=255)                               ggAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGTATCAACTTGCGAAACAACCGCTGCACAGCCAGGTGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAAcgcc                                                                                                                    <  1:573245‑M2/149‑5 (MQ=255)                                    ttttGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTa                                                                                                               >  3:53934/1‑149 (MQ=255)                                     tttGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑Gttagcgcagcaagggttttcgccaacatcgcccgtaac                                                                                                              <  1:349397‑M1/149‑39 (MQ=255)                                       tGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAAc                                                                                                            <  2:26445/149‑1 (MQ=255)                                       tGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAAc                                                                                                            <  2:568533/149‑1 (MQ=255)                                           aTTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGaa                                                                                                        >  1:243338/1‑149 (MQ=255)                                                 aaCGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAg                                                                                                  <  3:3674/149‑1 (MQ=255)                                                 aaCGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAg                                                                                                  >  2:455339/1‑149 (MQ=255)                                                 aaCGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAg                                                                                                  <  1:458000/149‑1 (MQ=255)                                                            ccGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAATCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTa                                                                                       <  4:534744/149‑1 (MQ=255)                                                                                ccgccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGtt                                                                   <  4:403605/149‑1 (MQ=255)                                                                                  gccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGa                                                                 >  1:438827/1‑149 (MQ=255)                                                                                   ccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTTCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAg                                                                         >  3:570504/1‑140 (MQ=255)                                                                                   ccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAg                                                                         <  4:570504/140‑1 (MQ=255)                                                                                   ccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGaa                                                                <  4:243157/149‑1 (MQ=255)                                                                                   ccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCATTGCTGCGCTAACGACCGGTAAACTGAAATACAGCTTCTTCTGTAATTGCGGCACCGTGTCCGTTGaa                                                                <  2:50305/149‑1 (MQ=255)                                                                                       aCAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAagcag                                                            >  1:428148/1‑149 (MQ=255)                                                                                         aGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAgcg                                                          >  1:179233/1‑149 (MQ=255)                                                                                               aaCTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAg                                                    >  4:260825/1‑149 (MQ=255)                                                                                                 cTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGaaa                                                                                                       >  4:254088/1‑96 (MQ=255)                                                                                                 cTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGaaa                                                                                                       <  3:254088/96‑1 (MQ=255)                                                                                                  ttGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTg                                                 <  3:545048/149‑1 (MQ=255)                                                                                                  ttGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTg                                                 >  3:340147/1‑149 (MQ=255)                                                                                                        aacaacCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGaaa                                           <  4:579373/149‑1 (MQ=255)                                                                                                        aacaacCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCCGAAGCTGGCGaaa                                           >  2:523780/1‑149 (MQ=255)                                                                                                         acaacCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATa                                                                                    >  3:407871/1‑107 (MQ=255)                                                                                                         acaacCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATa                                                                                    <  4:407871/107‑1 (MQ=255)                                                                                                            acCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCtt                                       <  3:99671/149‑1 (MQ=255)                                                                                                              cGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGATAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTAc                                     <  4:52763/149‑1 (MQ=255)                                                                                                                 tGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAg                                  <  3:260825/149‑1 (MQ=255)                                                                                                                    acaGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACa                                                                                                   >  4:98607/1‑81 (MQ=255)                                                                                                                    acaGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACa                                                                                                   <  3:98607/81‑1 (MQ=255)                                                                                                                      aGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCAcc                             >  1:514651/1‑149 (MQ=255)                                                                                                                          aGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACcgcgc                         >  4:48759/1‑149 (MQ=255)                                                                                                                                tgctCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAg                   >  3:146421/1‑149 (MQ=255)                                                                                                                                  ctCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGtt                 <  3:48759/149‑1 (MQ=255)                                                                                                                                   tCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGttt                >  1:33827/1‑149 (MQ=255)                                                                                                                                          gTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAAcgcccgtaacggatcgagcagctcctggctgtgcagcggttgtttcgcaagttgattctgtacggcggaaaccacaactggattacggtgccccacgttgaaaattccaaa         <  1:349397‑M2/149‑112 (MQ=255)                                                                                                                                                ggC‑‑‑Gttagcgcagcaagggttttcgccaacatcgcccgtaacggatcgagcagcacctggctgtgcagcggttgtttcgcaagttgatactgtacggcggaaaccacaactggattacggtgccccacgttgaaaattccaaaacctcc   <  1:573245‑M1/149‑146 (MQ=255)                                                                                                                                                ggC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGcca   >  4:229114/1‑149 (MQ=255)                                                                                                                                                ggC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGcca   <  1:402423/149‑1 (MQ=255)                                                                                                                                                ggC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCCGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGcca   <  1:523780/149‑1 (MQ=255)                                                                                                                                                 gC‑‑‑Gttagcgcagcaagggttttcgccaacatcgcccgtaacggatcgagcagctcctggctgtgcagcggttgtttcgcaagatgattctgtacggcggaaaccacaactggattacggtgccccacgttgaaaatcccaaaacctccc  >  1:243514‑M1/1‑3 (MQ=255)                                                                                                                                                 gC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAAcgcccgtaacggatcgagcagctcctggctgtgcagcggttgtttcgcaagttgattctgtacggcggaaaccacaactggattacggtgccccacgttgaaaattccaaaacctccc  >  2:349397‑M2/1‑31 (MQ=255)                                                                                                                                                      |                                                                                                                                                   ACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGC‑‑‑GATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCAC  >  NC_000913/3219728‑3220020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.