New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4286330 =136 (0.820)4 (0.020) 3/258 NT 2.9% coding (1042/1959 nt) acs acetyl‑CoA synthetase
?NC_000913 4286367 = 136 (0.830)coding (1005/1959 nt) acs acetyl‑CoA synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGATATCTACTGGTGCACCGCCGATGTGGGCTGGGTGACCGGACACAGTTACTTGCTGTACGGCCCGCTGGCCTGCGGTGCGACCACGCTGATGTTTGAAGGCGTACCCAACTGGCCGACGCCTGCCCGTATGGCGCAGGTGGTGGACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4286478‑4286330
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCAC  >  NC_000913/4286367‑4286466
                                                                                                                                                                                                                                                         
TGATATCTACTGGTGCACCGCCGATGTGGGCTGGGTGACCGGACACAGTTACTTGCTGTACGGCCCGCTGGCCTGCGGTGCGACCACGCTGATGTTTGAAGGCGTACCCAACTGGCCGACGCCTGCCCGTATGGCGCAGGTGGTGGACA                                                                                                      >  4:310066/1‑149
                                                                                                           CCAACTGGCCGACGCCTGCCCGTATGGCGCAGGTGGTGGACAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCAC  <  1:629780/142‑1
                                                                                                           CCAACTGGCCGACGCCTGCCCGTATGGCGCAGGTGGTGGACAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCAC  >  2:629780/1‑142
                                                                                                           CCAACTGGCCGACGCCTGCCCGTATGGCGCAGGTGGTGGACAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCG        <  3:1374774/136‑1
                                                                                                           CCAACTGGCCGACGCCTGCCCGTATGGCGCAGGTGGTGGACAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCG        >  4:1374774/1‑136
                                                                                                                                                                                                                                                         
TGATATCTACTGGTGCACCGCCGATGTGGGCTGGGTGACCGGACACAGTTACTTGCTGTACGGCCCGCTGGCCTGCGGTGCGACCACGCTGATGTTTGAAGGCGTACCCAACTGGCCGACGCCTGCCCGTATGGCGCAGGTGGTGGACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4286478‑4286330
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCAC  >  NC_000913/4286367‑4286466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.