mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | 25-36_S25_L001_R1_001_150209.good.fq | 903,704 | 228,898,449 | 100.0% | 253.3 bases | 275 bases | 98.5% |
| errors | 25-36_S25_L001_R1_001_150513.good.fq | 827,565 | 216,154,639 | 100.0% | 261.2 bases | 278 bases | 98.1% |
| errors | 25-36_S25_L001_R2_001_150209.good.fq | 903,703 | 229,193,633 | 100.0% | 253.6 bases | 275 bases | 91.2% |
| errors | 25-36_S25_L001_R2_001_150513.good.fq | 827,565 | 217,142,273 | 100.0% | 262.4 bases | 278 bases | 74.9% |
| total | 3,462,537 | 891,388,994 | 100.0% | 257.4 bases | 278 bases | 90.8% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 171.3 | 5.6 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 2788 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 231 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.028 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.81079 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 19:04:41 07 Jan 2019 | 19:05:48 07 Jan 2019 | 1 minute 7 seconds |
| Read alignment to reference genome | 19:05:48 07 Jan 2019 | 19:27:15 07 Jan 2019 | 21 minutes 27 seconds |
| Preprocessing alignments for candidate junction identification | 19:27:15 07 Jan 2019 | 19:28:10 07 Jan 2019 | 55 seconds |
| Preliminary analysis of coverage distribution | 19:28:10 07 Jan 2019 | 19:31:23 07 Jan 2019 | 3 minutes 13 seconds |
| Identifying junction candidates | 19:31:23 07 Jan 2019 | 19:31:29 07 Jan 2019 | 6 seconds |
| Re-alignment to junction candidates | 19:31:29 07 Jan 2019 | 19:35:36 07 Jan 2019 | 4 minutes 7 seconds |
| Resolving best read alignments | 19:35:36 07 Jan 2019 | 19:37:14 07 Jan 2019 | 1 minute 38 seconds |
| Creating BAM files | 19:37:14 07 Jan 2019 | 19:39:59 07 Jan 2019 | 2 minutes 45 seconds |
| Tabulating error counts | 19:39:59 07 Jan 2019 | 19:41:16 07 Jan 2019 | 1 minute 17 seconds |
| Re-calibrating base error rates | 19:41:16 07 Jan 2019 | 19:41:18 07 Jan 2019 | 2 seconds |
| Examining read alignment evidence | 19:41:18 07 Jan 2019 | 19:54:46 07 Jan 2019 | 13 minutes 28 seconds |
| Polymorphism statistics | 19:54:46 07 Jan 2019 | 19:54:46 07 Jan 2019 | 0 seconds |
| Output | 19:54:46 07 Jan 2019 | 19:55:05 07 Jan 2019 | 19 seconds |
| Total | 50 minutes 24 seconds | ||
