breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsGLY_26_025_S300_L001_R1_001.good.fq915,292127,928,145100.0%139.8 bases148 bases98.0%
errorsGLY_26_025_S300_L001_R2_001.good.fq915,292127,928,145100.0%139.8 bases148 bases97.9%
errorsGLY_26_025_S300_L002_R1_001.good.fq907,318127,715,152100.0%140.8 bases149 bases97.9%
errorsGLY_26_025_S300_L002_R2_001.good.fq907,318127,715,152100.0%140.8 bases149 bases97.7%
total3,645,220511,286,594100.0%140.3 bases149 bases97.9%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,652102.63.9100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000020794
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000182
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.012

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.71573

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input21:24:23 05 Jan 201921:25:16 05 Jan 201953 seconds
Read alignment to reference genome21:25:16 05 Jan 201921:32:36 05 Jan 20197 minutes 20 seconds
Preprocessing alignments for candidate junction identification21:32:36 05 Jan 201921:33:27 05 Jan 201951 seconds
Preliminary analysis of coverage distribution21:33:27 05 Jan 201921:35:59 05 Jan 20192 minutes 32 seconds
Identifying junction candidates21:35:59 05 Jan 201921:37:06 05 Jan 20191 minute 7 seconds
Re-alignment to junction candidates21:37:06 05 Jan 201921:39:28 05 Jan 20192 minutes 22 seconds
Resolving best read alignments21:39:28 05 Jan 201921:40:52 05 Jan 20191 minute 24 seconds
Creating BAM files21:40:52 05 Jan 201921:43:07 05 Jan 20192 minutes 15 seconds
Tabulating error counts21:43:07 05 Jan 201921:43:50 05 Jan 201943 seconds
Re-calibrating base error rates21:43:50 05 Jan 201921:43:52 05 Jan 20192 seconds
Examining read alignment evidence21:43:52 05 Jan 201921:59:30 05 Jan 201915 minutes 38 seconds
Polymorphism statistics21:59:30 05 Jan 201921:59:33 05 Jan 20193 seconds
Output21:59:33 05 Jan 201922:00:45 05 Jan 20191 minute 12 seconds
Total 36 minutes 22 seconds