breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsGLY_28_034_S316_L001_R1_001.good.fq1,724,216240,131,292100.0%139.3 bases149 bases98.2%
errorsGLY_28_034_S316_L001_R2_001.good.fq1,724,216240,131,292100.0%139.3 bases149 bases97.8%
errorsGLY_28_034_S316_L002_R1_001.good.fq1,690,335234,912,276100.0%139.0 bases149 bases98.1%
errorsGLY_28_034_S316_L002_R2_001.good.fq1,690,335234,912,276100.0%139.0 bases149 bases97.7%
total6,829,102950,087,136100.0%139.1 bases149 bases98.0%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,652183.53.9100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000042425
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000391
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.026

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.58367

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input13:25:38 06 Jan 201913:27:11 06 Jan 20191 minute 33 seconds
Read alignment to reference genome13:27:11 06 Jan 201913:40:46 06 Jan 201913 minutes 35 seconds
Preprocessing alignments for candidate junction identification13:40:46 06 Jan 201913:42:11 06 Jan 20191 minute 25 seconds
Preliminary analysis of coverage distribution13:42:11 06 Jan 201913:46:44 06 Jan 20194 minutes 33 seconds
Identifying junction candidates13:46:44 06 Jan 201913:50:02 06 Jan 20193 minutes 18 seconds
Re-alignment to junction candidates13:50:02 06 Jan 201913:54:42 06 Jan 20194 minutes 40 seconds
Resolving best read alignments13:54:42 06 Jan 201913:57:07 06 Jan 20192 minutes 25 seconds
Creating BAM files13:57:07 06 Jan 201914:01:15 06 Jan 20194 minutes 8 seconds
Tabulating error counts14:01:15 06 Jan 201914:02:40 06 Jan 20191 minute 25 seconds
Re-calibrating base error rates14:02:40 06 Jan 201914:02:41 06 Jan 20191 second
Examining read alignment evidence14:02:41 06 Jan 201914:50:02 06 Jan 201947 minutes 21 seconds
Polymorphism statistics14:50:02 06 Jan 201914:50:06 06 Jan 20194 seconds
Output14:50:06 06 Jan 201914:51:33 06 Jan 20191 minute 27 seconds
Total 1 hour 25 minutes 55 seconds