New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4218366 =97 (0.940)4 (0.040) 4/246 NT 4.2% coding (1258/1305 nt) aceA isocitrate lyase
?NC_000913 4218401 = NA (NA)coding (1293/1305 nt) aceA isocitrate lyase
Rejected: Frequency below/above cutoff threshold.

CTGTAGGCATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAAGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4218521‑4218366
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑actgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGG  >  NC_000913/4218401‑4218548
                                                                                                                                                                                                                                                                                                                
CTGTAGGCATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGA                                                                                                                                                             >  2:131306/1‑149
  GTAGGCATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACT                                                                                                                                                           <  3:714769/149‑1
     GGCATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAA                                                                                                                                                        <  1:494050/149‑1
     GGCATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAA                                                                                                                                                        >  2:107585/1‑149
     GGCATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAA                                                                                                                                                        <  2:298563/149‑1
     GGCATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAA                                                                                                                                                        <  2:507247/149‑1
       CATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAAGA                                                                                                                                                      <  1:764051/149‑1
       CATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCAGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAAGA                                                                                                                                                      <  3:762276/149‑1
                                                                                                                   TGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTT                                          >  1:661877/1‑149
                                                                                                                     CGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTAT                                        >  3:667416/1‑149
                                                                                                                      GATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGAT                                                            >  2:54329/1‑128
                                                                                                                      GATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGAT                                                            <  1:54329/128‑1
                                                                                                                                                    ACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTG         >  3:238567/1‑149
                                                                                                                                                    ACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTG         >  1:529271/1‑149
                                                                                                                                                     CTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTGGGCTGG        <  2:661877/149‑1
                                                                                                                                                      TGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGA       <  2:619173/149‑1
                                                                                                                                                          GAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTACACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGA       <  3:845671/145‑1
                                                                                                                                                          GAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTACACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGA       >  4:845671/1‑145
                                                                                                                                                           AATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGG  >  1:295084/1‑149
                                                                                                                                                           AATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGG  >  4:773140/1‑149
                                                                                                                                                                                                                                                                                                                
CTGTAGGCATGATAAGACGCGCAAGCGTCGCATCAGGCATCGTGCACCGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAGTCAGCAACGGTTGTTGTTGCTTAGAACTGCGATTCTTCAGTGGAGCCGGTCAGCGCGGTGACTGAAGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4218521‑4218366
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑actgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGG  >  NC_000913/4218401‑4218548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.