New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4218366 =97 (0.940)4 (0.040) 4/246 NT 4.2% coding (1258/1305 nt) aceA isocitrate lyase
?NC_000913 4218401 = NA (NA)coding (1293/1305 nt) aceA isocitrate lyase
Rejected: Frequency below/above cutoff threshold.

Only 100 of 111 total aligned reads displayed.

TGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGG  >  NC_000913/4218253‑4218548
                                                                                                                                                    |                                                                                                                                                   
tGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTgaagaa                                                                                                                                                     <  2:643257/149‑1 (MQ=255)
  cagcagCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCCCCGCGCTGACCGGCTCCACTGAAGAATc                                                                                                                                                   <  3:881487/149‑1 (MQ=255)
   ataagacgcgcaagcgtcgcatcaggcatcgtgcaccgattgccggatgcggcgtgaacgccttatccggcctacagtcagcaacggttgttgttgcttagaactgcgatTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCg                                                                                                                                                  >  3:667416‑M1/111‑149 (MQ=255)
   agcagcCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCg                                                                                                                                                  >  4:803982/1‑149 (MQ=255)
   agcagcCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCg                                                                                                                                                  >  4:518133/1‑149 (MQ=255)
     cagcCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCa                                                                                                                                                <  3:410183/149‑1 (MQ=255)
     cagcCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCa                                                                                                                                                <  1:99803/149‑1 (MQ=255)
     cagcCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCa                                                                                                                                                <  2:901553/149‑1 (MQ=255)
     aagacgcgcaagcgtcgcatcaggcatcgtgcaccgattgccggatgcggcgtgaacgccttatccggcctacagtcagcaacggttgttgttgcttagaactgcgatTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCa                                                                                                                                                >  1:661877‑M1/109‑149 (MQ=255)
            aaTTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTaa                                                                                                                                         <  3:353172/149‑1 (MQ=255)
            aaTTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGCCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTaa                                                                                                                                         <  2:801996/149‑1 (MQ=255)
             aTTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAg                                                                                                                                        >  3:739047/1‑149 (MQ=255)
                tGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGcaa                                                                                                                                     <  4:523254/149‑1 (MQ=255)
                tGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGcaa                                                                                                                                     <  4:698967/149‑1 (MQ=255)
                    gccgcGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGcaacaac                                                                                                                                 >  2:796562/1‑149 (MQ=255)
                       gcgAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCaacaacaac                                                                                                                              <  1:758288/149‑1 (MQ=255)
                       atcaggcatcgtgcaccgattgccggatgcggcgtgaacgccttatccggcctacagtcagcaacggttgttgttgcttagaactgcgatTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATc                                                                                                                                                   >  2:54329‑M1/91‑128 (MQ=255)
                       atcaggcatcgtgcaccgattgccggatgcggcgtgaacgccttatccggcctacagtcagcaacggttgttgttgcttagaactgcgatTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATc                                                                                                                                                   <  1:54329‑M1/38‑1 (MQ=255)
                         gAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCg                                                                                                                            >  1:314344/1‑149 (MQ=255)
                         gAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCg                                                                                                                            >  3:264409/1‑149 (MQ=255)
                           aaGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGtt                                                                                                                          <  2:320703/149‑1 (MQ=255)
                           aaGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGtt                                                                                                                          <  3:803982/149‑1 (MQ=255)
                             gATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGc                                                                                                                        <  3:499649/149‑1 (MQ=255)
                             gATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGc                                                                                                                        <  2:326942/149‑1 (MQ=255)
                                ggCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGa                                                                                                                     <  4:771856/149‑1 (MQ=255)
                                ggCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGa                                                                                                                     <  1:425138/149‑1 (MQ=255)
                                  cTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACt                                                                                                                   >  1:288685/1‑149 (MQ=255)
                                    ataCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGt                                                                                                                 >  1:123509/1‑149 (MQ=255)
                                     taCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTa                                                                                                                <  1:43033/149‑1 (MQ=255)
                                      aCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAg                                                                                                               <  3:500965/149‑1 (MQ=255)
                                         ttCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGcaa                                                                                                                                     >  4:894385/1‑124 (MQ=255)
                                         ttCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGcaa                                                                                                                                     <  3:894385/124‑1 (MQ=255)
                                         ttCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGcc                                                                                                            >  1:294335/1‑149 (MQ=255)
                                          tCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCg                                                                                                           <  4:360257/149‑1 (MQ=255)
                                          tCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCg                                                                                                           <  1:74266/149‑1 (MQ=255)
                                            gTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGa                                                                                                         <  1:495930/149‑1 (MQ=255)
                                               tctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGAtgc                                                                                                      >  3:306283/1‑147 (MQ=255)
                                               tctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATaa                                                                                                      >  4:837033/1‑149 (MQ=255)
                                               tctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATaa                                                                                                      <  2:328093/149‑1 (MQ=255)
                                               tctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATaa                                                                                                      <  2:321795/149‑1 (MQ=255)
                                                ctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAg                                                                                                     <  1:318559/149‑1 (MQ=255)
                                                 tcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAgg                                                                                                    >  4:431861/1‑149 (MQ=255)
                                                  cACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGc                                                                                                   >  1:66622/1‑149 (MQ=255)
                                                   aCCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCg                                                                                                  >  2:572002/1‑149 (MQ=255)
                                                      agcagGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGc                                                                                                             >  1:920473/1‑135 (MQ=255)
                                                      agcagGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGc                                                                                                             <  2:920473/135‑1 (MQ=255)
                                                      agcagGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTc                                                                                               <  3:584454/149‑1 (MQ=255)
                                                       gcagGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCa                                                                                              <  3:837033/149‑1 (MQ=255)
                                                       gcagGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCa                                                                                              >  2:901063/1‑149 (MQ=255)
                                                         agGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGtt                                                                                                <  2:427710/145‑1 (MQ=255)
                                                         agGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAcg                                                                                            >  1:681325/1‑149 (MQ=255)
                                                          ggAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAcgc                                                                                           <  3:162283/149‑1 (MQ=255)
                                                               tGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATGCGGCGTGAAcgccttat                                                                                      <  3:885175/149‑5 (MQ=255)
                                                                  gTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGGTGCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                   <  4:545662/149‑1 (MQ=255)
                                                                     cAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                >  1:373049/1‑149 (MQ=255)
                                                                        gTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTCCTAAGCAACAACAACCGATGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATc                                                                             >  4:551021/1‑149 (MQ=255)
                                                                             ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAc                                                                                             <  4:580874/128‑1 (MQ=255)
                                                                             ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAc                                                                                             >  3:580874/1‑128 (MQ=255)
                                                                             ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGc                                                                        >  4:172649/1‑149 (MQ=255)
                                                                             ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGc                                                                        >  3:154339/1‑149 (MQ=255)
                                                                             ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTACTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGc                                                                        >  3:78281/1‑149 (MQ=255)
                                                                              tCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCa                                                                       <  2:681325/149‑1 (MQ=255)
                                                                                  tAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGAt                                                                   <  2:512747/149‑1 (MQ=255)
                                                                                      gTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCt                                                               <  2:288685/149‑1 (MQ=255)
                                                                                         acgacTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGa                                                                                                                     >  3:402425/1‑92 (MQ=255)
                                                                                         acgacTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGa                                                                                                                     <  4:402425/92‑1 (MQ=255)
                                                                                              tattatTCAGGGCGGTACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGACCGGATAAGGCGTTCACGCCGCAGCCGGCAATCGGTGCACGACGCCTGATGCGAc                                                       >  2:721920/1‑149 (MQ=255)
                                                                                              tattatTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGAt                                                                                                        <  4:779649/100‑1 (MQ=255)
                                                                                              tattatTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGAt                                                                                                        >  3:779649/1‑100 (MQ=255)
                                                                                              tattatTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGAc                                                       >  1:107585/1‑149 (MQ=255)
                                                                                              tattatTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGAc                                                       >  1:506822/1‑149 (MQ=255)
                                                                                                  attCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCtt                                                   <  3:172649/149‑1 (MQ=255)
                                                                                                   ttCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCt                                                               >  2:396578/1‑136 (MQ=255)
                                                                                                   ttCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCt                                                               <  1:396578/136‑1 (MQ=255)
                                                                                                   ttCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTg                                                  >  4:607378/1‑149 (MQ=255)
                                                                                                   ttCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTCCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTg                                                  >  1:632865/1‑149 (MQ=255)
                                                                                                      aGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTgcgc                                               >  2:254758/1‑149 (MQ=255)
                                                                                                             cgattcttcagtggagccggtcagcgcggtgactgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTAt                                        >  3:667416‑M2/40‑149 (MQ=255)
                                                                                                              gattcttcagtggagccggtcagcgcggtgactgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGAt                                                            <  1:54329‑M2/90‑1 (MQ=255)
                                                                                                              gattcttcagtggagccggtcagcgcggtgactgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGAt                                                            >  2:54329‑M2/39‑128 (MQ=255)
                                                                                                              aCGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATc                                       >  3:481011/1‑149 (MQ=255)
                                                                                                              aCGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATc                                       <  3:6337/149‑1 (MQ=255)
                                                                                                                 TCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCTGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATg                                    <  3:762276‑M1/149‑1 (MQ=255)
                                                                                                                 TCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATg                                    <  1:764051‑M1/149‑1 (MQ=255)
                                                                                                                   TTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGcc                                  >  2:107585‑M1/1‑149 (MQ=255)
                                                                                                                   TTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGcc                                  <  1:494050‑M1/149‑1 (MQ=255)
                                                                                                                   TTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGcc                                  <  2:298563‑M1/149‑1 (MQ=255)
                                                                                                                      AGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTAc                               <  3:714769‑M1/149‑1 (MQ=255)
                                                                                                                        TCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAg                             >  2:131306‑M1/1‑149 (MQ=255)
                                                                                                                          aCCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGcc                           >  3:633875/1‑149 (MQ=255)
                                                                                                                                    ccGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAAc                 <  4:714769/149‑1 (MQ=255)
                                                                                                                                      ggCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGt               >  2:873844/1‑149 (MQ=255)
                                                                                                                                            actgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTg         >  1:529271‑M2/9‑149 (MQ=255)
                                                                                                                                            actgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTg         >  3:238567‑M2/9‑149 (MQ=255)
                                                                                                                                             ctgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTGGGCTgg        <  2:661877‑M2/142‑1 (MQ=255)
                                                                                                                                              tgaagaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa       <  2:619173‑M2/143‑1 (MQ=255)
                                                                                                                                                  gaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTACACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa       <  3:845671‑M2/143‑1 (MQ=255)
                                                                                                                                                  gaATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTACACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa       >  4:845671‑M2/3‑145 (MQ=255)
                                                                                                                                                   aATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAgg  >  4:773140‑M2/2‑149 (MQ=255)
                                                                                                                                                   aATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAgg  >  1:295084‑M2/2‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGG  >  NC_000913/4218253‑4218548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.