Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,293,015	IS1 (+) +8 bp	44.6%	intergenic (‑93/‑505)	hns ← / → tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	54 (0.460)	45/260	NT	46.2%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	1293015 =	59 (0.540)					intergenic (‑93/‑512)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
*	?	NC_000913	= 258675	NA (NA)	41 (0.350)	35/260	NT	39.5%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 1293022	59 (0.540)					intergenic (‑100/‑505)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

TAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/258532‑258675 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGAACAACATCCGTACTCTTCGTGCG  <  NC_000913/1293022‑1292875                                                                                                                                                                                                                                                                                                      TAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATT                                                                                                                                                 <  1:199617/149‑1                            CCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTG                                                                                                                      <  1:570201/149‑1                                     ACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTG                                                                                                              >  4:186758/1‑148                                      CCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAA                                                                                                            >  1:1026905/1‑149                                            CGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAA                                                                                                       <  4:604894/148‑1                                            CGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCC                                                                                                                               >  4:715068/1‑124                                            CGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCC                                                                                                                               <  3:715068/124‑1                                                 GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCTCCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTC                                                                                                  <  4:890181/148‑1                                                         ACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACCACTGAATTTAAGGCTCTATTATTAC                                                                                         <  2:165925/149‑1                                                                 GACAGCTGATAGAAACCGAAGCCACTGGAGCACATCAAAAAAACCATCATACACTAAATCAGTAAGTTGGCAGCATCAACCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGACTTTAAGGCTCTATTATTAACTCAACc                                                                                  >  4:501252/1‑147                                                                        GATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACC                                                                           >  3:341797/1‑148                                                                          aAGAAACAGAAGCCACTGGAGCACCTCAAAAACA‑CATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCA                                                                        <  3:603069/147‑1                                                                           AGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAA                                                                       <  2:1026905/149‑1                                                                            GAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAA                                                                       >  3:779497/1‑148                                                                             AAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATA                                                                     >  2:608282/1‑149                                                                                CAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAA                                                                  <  1:1041842/149‑1                                                                                CAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAA                                                                  <  1:820105/149‑1                                                                                CAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATA                                                                   <  3:6388/148‑1                                                                                        ACTGGAGCACCTCAATAACACCATCATACACTAAATCGGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGA                                                          >  2:835339/1‑149                                                                                              GCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTA                                                    >  2:634300/1‑149                                                                                                  CTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAAT                                                >  2:550384/1‑149                                                                                                   TCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAGTATAAGTTTGAGATTACTACAAT                                                >  3:280233/1‑148                                                                                                   TCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATG                                               >  2:200599/1‑149                                                                                                   TCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATG                                               >  2:66463/1‑149                                                                                                     AAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAG                                             >  1:490181/1‑149                                                                                                     AAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGA                                              >  4:217348/1‑148                                                                                                         ACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAA                                         <  1:608282/149‑1                                                                                                         ACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAA                                                                                <  3:480914/110‑1                                                                                                         ACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAA                                                                                >  4:480914/1‑110                                                                                                             CATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATT                                                                                              <  1:854056/92‑1                                                                                                             CATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATT                                                                                              >  2:854056/1‑92                                                                                                              ATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTC                                                                                                  >  3:522154/1‑87                                                                                                              ATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTC                                                                                                  <  4:522154/87‑1                                                                                                                     ACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATT                             >  1:1011727/1‑149                                                                                                                     ACTAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATT                             >  2:457463/1‑149                                                                                                                       TAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCT                           >  1:417184/1‑149                                                                                                                       TAAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTC                            <  3:304527/148‑1                                                                                                                        AAATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCT                           >  3:201332/1‑148                                                                                                                          ATCAGTAAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGA                         <  4:341797/148‑1                                                                                                                                AAGTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGAACAACA                   <  3:597954/148‑1                                                                                                                                  GTTGGCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGAACAACATC                 >  3:991674/1‑148                                                                                                                                      GCAGCATCACCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGAACAACATCCGTAC            <  1:457463/149‑1                                                                                                                                               CCCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGAACAACATCCGTACTCTTCGTGC   >  1:120912/1‑149                                                                                                                                                CCAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGAACAACATCCGTACTCTTCGTGCG  >  2:870676/1‑149                                                                                                                                                                                                                                                                                                      TAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/258532‑258675 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAATTTTGAATTCCTTACATTCCTGGCTATTGCACAACTGAATTTAAGGCTCTATTATTACCTCAACAAACCACCCCAATATAAGTTTGAGATTACTACAATGAGCGAAGCACTTAAAATTCTGAACAACATCCGTACTCTTCGTGCG  <  NC_000913/1293022‑1292875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.