Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 1,293,015 IS1 (+) +8 bp 44.6% intergenic (‑93/‑505) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)54 (0.460) 45/260 NT 46.2% noncoding (768/768 nt) IS1 repeat region
?NC_000913 1293015 = 59 (0.540)intergenic (‑93/‑512) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 258675NA (NA)41 (0.350) 35/260 NT 39.5% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 1293022 59 (0.540)intergenic (‑100/‑505) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

TTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGAC  >  NC_000913/257768‑257916
                                                                                                                                            |        
ttAAAAATTTGCTAAATTTTGCCAATTTGATAAAACAGGTGCATAACAACAGGAGCTAGCAATGACGTTACCGAGTGGACACCATAAGAGCAGATTGATCAAAAAAATTACCGCACTACGCCCGTATAATCGCGAaggtaagtgcaaag  >  2:969847/1‑143 (MQ=255)
                                                                                                                                            |        
TTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGAC  >  NC_000913/257768‑257916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.