New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4201782 =81 (0.940)10 (0.140) 9/164 8.3 13.4% intergenic (‑94/‑144) zraP/zraS Zn‑dependent periplasmic chaperone/sensory histidine kinase in two‑component regulatory system with ZraR
?NC_000913 4201828 = 62 (0.870)intergenic (‑140/‑98) zraP/zraS Zn‑dependent periplasmic chaperone/sensory histidine kinase in two‑component regulatory system with ZraR
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

TTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGAT  >  NC_000913/4201730‑4201927
                                                                                                  |                                                                                                   
ttCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCAt                                                                                                    <  2:830768/100‑1 (MQ=255)
 tCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCAtt                                                                                                   >  2:1847035/1‑100 (MQ=255)
  ccGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTg                                                                                                  >  2:893626/1‑100 (MQ=255)
  ccGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTg                                                                                                  <  2:471053/100‑1 (MQ=255)
    gTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGaa                                                                                                <  1:2080150/100‑1 (MQ=255)
       ccAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACt                                                                                             >  1:182400/1‑100 (MQ=255)
            gATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGc                                                                                        >  2:478389/1‑100 (MQ=255)
                aaaCGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCttt                                                                                    >  2:1325400/1‑100 (MQ=255)
                 aaCGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTtg                                                                                   <  1:1055364/100‑1 (MQ=255)
                   cGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTtgtg                                                                                 <  2:1608780/100‑1 (MQ=255)
                     cTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGtt                                                                               <  1:1770189/100‑1 (MQ=255)
                     cTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGtt                                                                               >  1:2004385/1‑100 (MQ=255)
                     cTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGtt                                                                               <  2:183802/100‑1 (MQ=255)
                       gATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTAc                                                                             >  2:143619/1‑100 (MQ=255)
                         tatGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAg                                                                           >  1:500863/1‑100 (MQ=255)
                           tGACGGGTAATCTGGCATGATAAACGAGTAAAAATGCCTCGCCTGCTGCGGGTAGCGAGGCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTa                                                                              >  2:807953/1‑95 (MQ=255)
                           tGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAgcg                                                                         >  1:1600898/1‑100 (MQ=255)
                           tGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAgcg                                                                         >  2:2187266/1‑100 (MQ=255)
                             aCGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCa                                                                       >  1:1481384/1‑100 (MQ=255)
                                 tctaTCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGt                                                                   <  2:215423/97‑1 (MQ=255)
                                 gTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGt                                                                   <  1:739549/100‑1 (MQ=255)
                                 gTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGt                                                                   <  1:533720/100‑1 (MQ=255)
                                 gTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGt                                                                   <  1:558417/100‑1 (MQ=255)
                                    aTCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTaga                                                                >  1:215422/1‑98 (MQ=255)
                                      cTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAgcg                                                              <  1:2281953/100‑1 (MQ=255)
                                       tGGCATGATAAACGAGTAAAAATGCCTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAgcgc                                                             <  1:1410839/100‑1 (MQ=255)
                                       tGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAgcgc                                                             <  1:379161/100‑1 (MQ=255)
                                        ggCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCt                                                            <  1:1990186/100‑1 (MQ=255)
                                         gCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTg                                                           >  1:1488216/1‑100 (MQ=255)
                                         gCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTg                                                           >  2:1186040/1‑100 (MQ=255)
                                           agtttcaatgAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGAt                                                         <  1:1269708/91‑1 (MQ=255)
                                           aTGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGAt                                                         >  1:674429/1‑100 (MQ=255)
                                                aaaCGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAg                                                    <  2:528633/100‑1 (MQ=255)
                                                aaaCGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAg                                                    >  1:924096/1‑100 (MQ=255)
                                                aaaCGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAg                                                    >  1:668657/1‑100 (MQ=255)
                                                 aaCGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGa                                                   >  1:1267362/1‑100 (MQ=255)
                                                   cGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATg                                                 >  1:1728195/1‑100 (MQ=255)
                                                    gAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATgg                                                >  2:1731396/1‑100 (MQ=255)
                                                    gAGGAAAAATGAGTCGCGTGCTGGGGGTGGTGAGTCATGTTTACTGATTGAAACTTAAGCCTTGGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATg                                                 <  2:1451351/99‑1 (MQ=255)
                                                     aGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGc                                               >  2:2070960/1‑100 (MQ=255)
                                                      gTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCa                                              >  1:1895306/1‑100 (MQ=255)
                                                       tAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCAt                                             <  2:1998820/100‑1 (MQ=255)
                                                        aaaaaTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATg                                            >  2:2207346/1‑100 (MQ=255)
                                                         aaaaTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGa                                           <  2:1806296/100‑1 (MQ=255)
                                                          aaaTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGAt                                          >  1:1001235/1‑100 (MQ=255)
                                                          aaaTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGAt                                          <  1:1325403/100‑1 (MQ=255)
                                                               aCTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctg                                     <  1:1166353/100‑1 (MQ=255)
                                                               aCTCGCCTGCTGCGGGTAGCGAGTCATTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctgc                                    <  2:895056/100‑1 (MQ=255)
                                                                cTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctgc                                    >  2:1845847/1‑100 (MQ=255)
                                                                  cGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctgctg                                  >  1:490969/1‑100 (MQ=255)
                                                                  cGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctgctg                                  <  1:1960034/100‑1 (MQ=255)
                                                                  cGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctgctg                                  >  2:1465997/1‑100 (MQ=255)
                                                                  cGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctgctg                                  <  2:647411/100‑1 (MQ=255)
                                                                    ccTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTc                                >  1:1387700/1‑100 (MQ=255)
                                                                    ccTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTc                                <  1:70641/100‑1 (MQ=255)
                                                                     ctgctgCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCa                               <  1:124900/100‑1 (MQ=255)
                                                                      tgctgcGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAg                              <  2:472784/100‑1 (MQ=255)
                                                                        ctgcGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGaa                            >  1:564721/1‑100 (MQ=255)
                                                                           cGGGTAGCGAGTCATTTTTACTCATTGAATCTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGCTAAAAGATGGCATGATTTCTGCTGTCAGAAAgg                         <  2:1387697/100‑1 (MQ=255)
                                                                            gggTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAggg                        <  2:924094/100‑1 (MQ=255)
                                                                            gggTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAggg                        <  2:1481380/100‑1 (MQ=255)
                                                                              gTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGAt                      <  1:2009895/100‑1 (MQ=255)
                                                                               tAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATg                     <  2:298286/100‑1 (MQ=255)
                                                                                   gAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCa                 >  2:1659289/1‑100 (MQ=255)
                                                                                     gTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGGATGATTTCTGCTGTCAGAAAGGGATGAGCAgg               <  1:2187276/100‑1 (MQ=255)
                                                                                      tcttccgatcTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGc              <  1:29657/91‑1 (MQ=255)
                                                                                      tCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGc              <  2:1001233/100‑1 (MQ=255)
                                                                                       cATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCa             <  1:1847042/100‑1 (MQ=255)
                                                                                        aTTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGcn             <  2:512696/99‑2 (MQ=255)
                                                                                        aTTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCaa            >  1:2097838/1‑100 (MQ=255)
                                                                                         ttccgatctaTTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCaaa           <  1:2111041/91‑1 (MQ=255)
                                                                                          ttttACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAaag          >  1:1650471/1‑100 (MQ=255)
                                                                                             tACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAaagaag       <  2:1269705/100‑1 (MQ=255)
                                                                                               cTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAaagaagaa     >  1:1219492/1‑100 (MQ=255)
                                                                                                tCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAaagaagaag    <  2:674428/100‑1 (MQ=255)
                                                                                                tCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAaagaagaag    <  2:779221/100‑1 (MQ=255)
                                                                                                 cATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCagatcggaaga   >  2:29657/1‑90 (MQ=255)
                                                                                                 cATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAagaagaaga   <  2:1267359/100‑1 (MQ=255)
                                                                                                  aTTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCaaaagatcggaa  >  2:2111033/1‑91 (MQ=255)
                                                                                                  aTTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGAt  >  2:1946185/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGAT  >  NC_000913/4201730‑4201927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.