breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 843,749 | C→A | D578Y (GAT→TAT) | ybiO ← | mechanosensitive channel protein, intermediate conductance |
RA | 1,331,039 | A→C | intergenic (‑371/‑9) | yciN ← / → topA | DUF2498 protein YciN/DNA topoisomerase I, omega subunit |
MC JC | 1,680,573 | Δ814 bp | [ydgI]–[folM] | [ydgI], [folM] | |
RA | 1,756,529 | A→T | T278S (ACC→TCC) | pykF → | pyruvate kinase I |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,750,720 | C→T | intergenic (‑12/‑111) | grpE ← / → nadK | heat shock protein/NAD kinase |
RA | 2,810,759 | G→A | intergenic (+80/‑11) | ygaH → / → mprA | putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
JC | 3,815,859 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
RA | 4,072,171 | C→A | V161F (GTC→TTC) | yihT ← | 6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase |
RA | 4,078,996 | G→T | intergenic (+558/‑301) | yiiD → / → yiiE | GNAT family putative N‑acetyltransferase/CopG family putative transcriptional regulator |
RA | 4,189,448 | C→T | Q1367* (CAG→TAG) | rpoC → | RNA polymerase, beta prime subunit |
JC | 4,215,179 | Δ4 bp | coding (900‑903/930 nt) | metA → | homoserine O‑transsuccinylase |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3423715–3424234 | 3424592–3424238 | 5–878 | 57 [52] | [53] 56 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 24 (0.240) | 46 (0.550) | 36/166 | 2.3 | 68.6% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 22 (0.260) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 24 (0.240) | 74 (0.880) | 47/166 | 1.0 | 77.9% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 22 (0.260) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 101 (1.050) | 73/190 | 0.1 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |