breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 843,749 C→A D578Y (GAT→TAT)  ybiO ← mechanosensitive channel protein, intermediate conductance
RA 1,331,039 A→C intergenic (‑371/‑9) yciN ← / → topA DUF2498 protein YciN/DNA topoisomerase I, omega subunit
MC JC 1,680,573 Δ814 bp [ydgI][folM] [ydgI], [folM]
RA 1,756,529 A→T T278S (ACC→TCC)  pykF → pyruvate kinase I
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,750,720 C→T intergenic (‑12/‑111) grpE ← / → nadK heat shock protein/NAD kinase
RA 2,810,759 G→A intergenic (+80/‑11) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 4,072,171 C→A V161F (GTC→TTC)  yihT ← 6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase
RA 4,078,996 G→T intergenic (+558/‑301) yiiD → / → yiiE GNAT family putative N‑acetyltransferase/CopG family putative transcriptional regulator
RA 4,189,448 C→T Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit
JC 4,215,179 Δ4 bp coding (900‑903/930 nt) metA → homoserine O‑transsuccinylase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423715–3424234 3424592–3424238 5–878 57 [52] [53] 56 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =24 (0.240)46 (0.550) 36/166 2.3 68.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 22 (0.260)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780524 (0.240)74 (0.880) 47/166 1.0 77.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 22 (0.260)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)101 (1.050) 73/190 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein