Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,242,942	Δ66 bp	coding (510‑575/891 nt)	malG ←	maltose transporter subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4242941	2 (0.020)	56 (0.750)	42/184	1.1	96.7%	coding (576/891 nt)	malG	maltose transporter subunit
	?	NC_000913	4243008 =	2 (0.030)					coding (509/891 nt)	malG	maltose transporter subunit

AATGGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4242836‑4242941 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGA  >  NC_000913/4243008‑4243104                                                                                                                                                                                                             AATGGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCC                                                                                                         >  1:528781/1‑100  ATGGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCA                                                                                                        >  1:1753388/1‑100    GGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGC                                                                                                      >  2:252730/1‑100       AGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGTCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCA                                                                                                   <  2:1603359/100‑1          GATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATC                                                                                                >  1:1157916/1‑100          GATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATC                                                                                                >  1:967099/1‑100           ATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCC                                                                                               <  2:1352714/100‑1             AAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCA                                                                                             <  2:335436/100‑1               ACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCAC                                                                                            <  1:1833492/99‑1                 GACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCA                                                                                         <  2:162555/100‑1                     GGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATA                                                                                     >  1:1487726/1‑100                      GATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATAC                                                                                    >  2:313379/1‑100                        TGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGC                                                                                  >  2:172553/1‑100                          AATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGA                                                                                >  2:586585/1‑100                             ACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAA                                                                             >  2:1590185/1‑100                             ACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAA                                                                             <  2:170943/100‑1                               CACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATT                                                                           <  1:1010426/100‑1                                 CCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTAC                                                                         >  1:1903048/1‑100                                    CCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCC                                                                      >  2:1668189/1‑100                                            GGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAG                                                              <  2:131533/100‑1                                              TACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTA                                                            <  1:1520692/100‑1                                               ACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTAT                                                           <  2:846947/100‑1                                                CTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGcg                                                              >  1:890079/1‑94                                                 TGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGCGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTC                                                         <  2:140299/100‑1                                                   ACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAG                                                       >  2:502411/1‑100                                                    CAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGG                                                      >  2:206572/1‑100                                                      GCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCC                                                    <  2:1775192/100‑1                                                          CAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATG                                                >  2:1541137/1‑100                                                          CAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATG                                                >  2:1012251/1‑100                                                            ACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAA                                              >  2:1548676/1‑100                                                             CAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAAT                                             >  1:1460525/1‑100                                                             CAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAA                                              >  1:1078923/1‑99                                                              AGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATG                                            <  1:362741/100‑1                                                              AGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATG                                            <  1:1763602/100‑1                                                               GGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATG                                            <  2:1276620/99‑1                                                                 ACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAA                                         <  2:1903061/100‑1                                                                  CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        >  2:252214/1‑100                                                                  CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        <  2:1305457/100‑1                                                                  CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        >  2:453887/1‑100                                                                  CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        >  2:875381/1‑100                                                                  CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        >  2:1062899/1‑100                                                                    AGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGT                                      >  1:999604/1‑100                                                                      GCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTAC                                    <  2:1227519/100‑1                                                                       CGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACT                                   >  2:704654/1‑100                                                                       CGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACT                                   <  2:1024719/100‑1                                                                       CGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACT                                   <  1:586583/100‑1                                                                       CGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACT                                   >  1:583047/1‑100                                                                              CCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAG                            >  2:1590737/1‑100                                                                              CCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAG                            >  2:1591730/1‑100                                                                                 GCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACG                         <  1:1631474/100‑1                                                                                   CACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATTCGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACG                         <  1:29041/98‑1                                                                                   CACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGAT                       <  1:1692813/100‑1                                                                                   CACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGAT                       <  1:172552/100‑1                                                                                        TGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAAC                  <  1:1012250/100‑1                                                                                        TGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAAC                  >  2:1440996/1‑100                                                                                            GCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACG              <  2:1401348/100‑1                                                                                             CACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGC             <  1:876019/100‑1                                                                                             CACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGC             <  1:1673106/100‑1                                                                                              ACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCA            <  1:252728/100‑1                                                                                                 ATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATAC         <  2:585421/100‑1                                                                                                     AGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACG     <  2:521074/100‑1                                                                                                      GCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGC    <  2:577119/100‑1                                                                                                       CGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCG   >  1:1909685/1‑100                                                                                                       CGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCG   >  2:1052789/1‑100                                                                                                       CGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCG   >  2:1209488/1‑100                                                                                                        GCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGA  <  2:749588/100‑1                                                                                                                                                                                                             AATGGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4242836‑4242941 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGA  >  NC_000913/4243008‑4243104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.