New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4526710 =83 (0.910)4 (0.050) 4/162 NT 5.2% coding (179/783 nt) sgcR putative DNA‑binding transcriptional regulator
?NC_000913 4526743 = 69 (0.830)coding (146/783 nt) sgcR putative DNA‑binding transcriptional regulator
Rejected: Frequency below cutoff threshold.

CAGCAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCCTGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4526816‑4526710
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcctggaTACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCAC  >  NC_000913/4526743‑4526841
                                                                                                                                                                                                              
CAGCAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTT                                                                                                            <  2:2203752/100‑1
CAGCAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTT                                                                                                            >  1:2332871/1‑100
  GCAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGC                                                                                                          >  2:2505681/1‑100
  GCAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGC                                                                                                          >  2:1547090/1‑100
  GCAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTG                                                                                                           <  1:1217333/99‑1
   CAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCC                                                                                                         <  2:1971731/100‑1
   CAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCC                                                                                                         <  1:824470/100‑1
    AGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCCT                                                                                                        >  1:405899/1‑100
     GGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCCTG                                                                                                       >  2:2537184/1‑100
      GCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCCTGG                                                                                                      <  2:114067/100‑1
       CCATGGAGCTTTTTGGCTGTGCGTAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGACACGGTTGCCT                                                                                                        <  2:737897/97‑1
                                                                 CAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCCTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAA                                           >  1:702158/1‑100
                                                                   ACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCCTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGC                                         >  1:602876/1‑100
                                                                        TATCCAGGCATGATCCGCGGTCACGGTTGCCTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCAT                                    >  1:21642/1‑100
                                                                         ATCCAGGCATGATCCGCGGTCACGGTTGCCTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCACAAAGCTCCATG                                   >  1:1188554/1‑100
                                                                                                     CCTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTn       <  2:1160578/100‑2
                                                                                                      caGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTG      <  1:1322445/98‑1
                                                                                                      CTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTG      >  1:1977933/1‑100
                                                                                                          ATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCAC  <  1:2092933/100‑1
                                                                                                          ATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCAC  <  1:202449/100‑1
                                                                                                                                                                                                              
CAGCAGGCCATGGAGCTTTTTGGCTATGCGGAAGCGACTGTCCGTCGTGATTTCCAGTATATTGTCAACCAGTATCCAGGCATGATCCGCGGTCACGGTTGCCTGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4526816‑4526710
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcctggaTACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCAC  >  NC_000913/4526743‑4526841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.