mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | qtrim-IND_KHP_HOT_A12_F056_I1_R1_S49_L004_R1_001 | 1,305,767 | 130,536,215 | 100.0% | 100.0 bases | 100 bases | 96.6% |
| errors | qtrim-IND_KHP_HOT_A12_F056_I1_R1_S49_L004_R2_001 | 1,305,756 | 130,310,154 | 100.0% | 99.8 bases | 100 bases | 96.1% |
| total | 2,611,523 | 260,846,369 | 100.0% | 99.9 bases | 100 bases | 96.4% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 53.8 | 1.6 | 98.1% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 51623 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 339 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.015 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.78048 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.8 |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum coverage each strand | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 16:04:19 04 Jan 2017 | 16:04:47 04 Jan 2017 | 28 seconds |
| Read alignment to reference genome | 16:04:48 04 Jan 2017 | 16:06:19 04 Jan 2017 | 1 minute 31 seconds |
| Preprocessing alignments for candidate junction identification | 16:06:19 04 Jan 2017 | 16:07:15 04 Jan 2017 | 56 seconds |
| Preliminary analysis of coverage distribution | 16:07:15 04 Jan 2017 | 16:08:17 04 Jan 2017 | 1 minute 2 seconds |
| Identifying junction candidates | 16:08:17 04 Jan 2017 | 16:09:20 04 Jan 2017 | 1 minute 3 seconds |
| Re-alignment to junction candidates | 16:09:20 04 Jan 2017 | 16:09:37 04 Jan 2017 | 17 seconds |
| Resolving alignments with junction candidates | 16:09:37 04 Jan 2017 | 16:11:09 04 Jan 2017 | 1 minute 32 seconds |
| Creating BAM files | 16:11:09 04 Jan 2017 | 16:11:46 04 Jan 2017 | 37 seconds |
| Tabulating error counts | 16:11:46 04 Jan 2017 | 16:12:54 04 Jan 2017 | 1 minute 8 seconds |
| Re-calibrating base error rates | 16:12:54 04 Jan 2017 | 16:12:54 04 Jan 2017 | 0 seconds |
| Examining read alignment evidence | 16:12:54 04 Jan 2017 | 16:21:47 04 Jan 2017 | 8 minutes 53 seconds |
| Polymorphism statistics | 16:21:47 04 Jan 2017 | 16:21:47 04 Jan 2017 | 0 seconds |
| Output | 16:21:47 04 Jan 2017 | 16:22:33 04 Jan 2017 | 46 seconds |
| Total | 18 minutes 13 seconds | ||
