breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 36,231 T→C intergenic (‑69/+40) caiD ← / ← caiC carnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase
RA 106,554 A→G intergenic (+98/‑3) ftsZ → / → lpxC GTP‑binding tubulin‑like cell division protein/UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
RA 139,187 T→C N680S (AAT→AGT)  gcd ← glucose dehydrogenase
RA 162,973 G→A C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase
JC 190,553 Δ14 bp coding (680‑693/726 nt) rpsB → 30S ribosomal subunit protein S2
RA 216,568 C→T A146T (GCC→ACC)  yaeF ← putative lipoprotein
RA 236,541 C→T R159* (CGA→TGA)  dnaQ → DNA polymerase III epsilon subunit
RA 254,727 A→C G330G (GGT→GGG pepD ← aminoacyl‑histidine dipeptidase (peptidase D)
MC JC 257,908 Δ776 bp [crl] [crl]
RA 318,690 A→G S438P (TCA→CCA)  rclA ← reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
RA 331,668 A→G intergenic (+172/‑703) betT → / → yahA choline transporter of high affinity/c‑di‑GMP‑specific phosphodiesterase
RA 338,334 T→A S4T (TCA→ACA)  yahG → DUF1116 family protein
RA 339,856 T→C intergenic (+113/‑309) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 344,961 T→C N262N (AAT→AAC yahL → uncharacterized protein
RA 401,440 T→G S19A (TCG→GCG)  iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
RA 426,040 A→T T344S (ACG→TCG)  queA → S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase
RA 428,188 G→T R181L (CGC→CTC)  secD → SecYEG protein translocase auxillary subunit
RA 432,771 T→G R27R (CGA→CGC yajI ← putative lipoprotein
RA 497,139 (T)5→6 intergenic (+145/‑36) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
RA 520,993 T→G V193G (GTC→GGC)  ybbP → putative ABC transporter permease
RA 524,866 A→G T536A (ACC→GCC)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 555,549 T→C R313R (CGT→CGC cysS → cysteinyl‑tRNA synthetase
RA 587,625 A→T A119A (GCT→GCA ybcH ← PRK09936 family protein
RA 658,153 A→G pseudogene (123/195 nt) ybeM → pseudogene, C‑N hydrolase superfamily homology;putative enzyme; Not classified; putative amidase
RA 676,403 C→T A49T (GCC→ACC)  ybeQ ← Sel1 family TPR‑like repeat protein
RA 700,805 A→T C264S (TGC→AGC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 720,946 C→T intergenic (+106/+110) ybfK → / ← kdpE uncharacterized protein/response regulator in two‑component regulatory system with KdpD
RA 753,654 G→A A272V (GCG→GTG)  gltA ← citrate synthase
RA 772,209 C→A T251N (ACC→AAC)  cydA → cytochrome d terminal oxidase, subunit I
RA 796,681 C→A A43D (GCC→GAC)  modC → molybdate ABC transporter ATPase; chlorate resistance protein
RA 796,704 C→T Q51* (CAA→TAA)  modC → molybdate ABC transporter ATPase; chlorate resistance protein
RA 801,422 G→A A222T (GCA→ACA)  ybhH → putative PrpF family isomerase
RA 849,405 G→A T231T (ACC→ACT rhtA ← threonine and homoserine efflux system
RA 868,748 Δ1 bp coding (1229/1872 nt) gsiA → glutathione ABC transporter ATPase
RA 873,807 G→A G277S (GGT→AGT)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 875,714 T→G V127G (GTC→GGC)  yliF → putative membrane‑anchored diguanylate cyclase
RA 882,722 G→A R5C (CGC→TGC)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
RA 894,155 T→G D124E (GAT→GAG potF → putrescine ABC transporter periplasmic binding protein
RA 914,948 A→G intergenic (‑91/+404) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 1,034,326 A→G N358S (AAC→AGC)  hyaB → hydrogenase 1, large subunit
RA 1,093,803 (A)6→5 coding (928/1359 nt) ycdT → diguanylate cyclase, membrane‑anchored
RA 1,100,233 G→A intergenic (+39/‑63) ycdY → / → ycdZ redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein
RA 1,105,806 T→C intergenic (+81/‑14) ymdA → / → ymdB uncharacterized protein/O‑acetyl‑ADP‑ribose deacetylase; RNase III inhibitor during cold shock; putative cardiolipin synthase C regulatory subunit
RA 1,127,049 A→G T82A (ACG→GCG)  yceM → putative oxidoreductase
RA 1,143,125 C→T D415N (GAC→AAC)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,188,434 A→G V448A (GTA→GCA)  phoQ ← sensory histidine kinase in two‑component regulatory system with PhoP
RA 1,197,739 A→G S167P (TCC→CCC)  ymfE ← e14 prophage; putative inner membrane protein
RA 1,264,376 T→C I221I (ATT→ATC hemA → glutamyl tRNA reductase
RA 1,292,944 T→C intergenic (‑22/‑583) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,303,332 T→G V145G (GTG→GGG)  oppB → oligopeptide ABC transporter permease
RA 1,369,921 A→G E78G (GAG→GGG)  pspE → thiosulfate:cyanide sulfurtransferase (rhodanese)
RA 1,390,147 G→A A151V (GCC→GTC)  mpaA ← murein peptide amidase A
RA 1,401,718 A→C V19G (GTC→GGC)  abgT ← p‑aminobenzoyl‑glutamate transporter; membrane protein
RA 1,401,844 A→G I471T (ATC→ACC)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit
RA 1,414,756 (T)6→5 coding (38/810 nt) recT ← Rac prophage; recombination and repair protein
RA 1,420,196 T→C N16D (AAC→GAC)  racR ← Rac prophage; putative DNA‑binding transcriptional regulator
RA 1,434,402 T→C intergenic (‑178/+556) ynaE ← / ← ttcC cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related
RA 1,446,972 Δ1 bp coding (312/906 nt) feaR ← transcriptional activator for tynA and feaB
RA 1,462,065 T→G G391G (GGT→GGG paaJ → 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
RA 1,475,315 G→T V58L (GTG→TTG)  ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,501,912 T→A I117I (ATT→ATA tehB → tellurite, selenium methyltransferase, SAM‑dependent; tellurite, selenium resistance protein
RA 1,505,561 T→C M174V (ATG→GTG)  ydcO ← BenE family inner membrane putative transporter
RA 1,519,159 A→T T45S (ACA→TCA)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent
RA 1,530,076 (A)7→8 coding (155/483 nt) ydcD → putative immunity protein for RhsE
RA 1,553,128 T→C V237V (GTA→GTG adhP ← ethanol‑active dehydrogenase/acetaldehyde‑active reductase
RA 1,560,731 Δ1 bp coding (199/1023 nt) ddpB ← D,D‑dipeptide ABC transporter permease
RA 1,580,094 C→T D233N (GAT→AAT)  ydeM ← putative YdeN‑specific sulfatase‑maturating enzyme
RA 1,642,062 +T coding (208/213 nt) cspF → Qin prophage; cold shock protein
RA 1,648,375 T→C intergenic (+34/‑133) dicA → / → ydfA Qin prophage; putative regulator for DicB/Qin prophage; uncharacterized protein
RA 1,665,195 (T)7→8 intergenic (+75/‑120) dmsD → / → clcB twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter
RA 1,673,475 A→G G9G (GGT→GGC mdtJ ← multidrug efflux system transporter
RA 1,681,932 T→C P33P (CCA→CCG ydgC ← GlpM family inner membrane protein
RA 1,701,585 Δ1 bp coding (629/1173 nt) malY → PLP‑dependent beta‑cystathionase and maltose regulon regulator
RA 1,703,693 C→T A206T (GCG→ACG)  ydgJ ← putative oxidoreductase
RA 1,712,306 (T)7→8 intergenic (+148/‑463) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
RA 1,712,518 (C)6→5 intergenic (+360/‑251) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
RA 1,728,444 C→A T33N (ACC→AAC)  rnt → RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair
RA 1,778,470 A→G Y278H (TAC→CAC)  ydiP ← putative DNA‑binding transcriptional regulator
RA 1,785,283 Δ1 bp coding (1830/2379 nt) ppsA ← phosphoenolpyruvate synthase
RA 1,800,755 C→T V606I (GTT→ATT)  thrS ← threonyl‑tRNA synthetase
RA 1,827,031 T→A D410V (GAT→GTT)  astB ← succinylarginine dihydrolase
RA 1,870,998 (T)6→5 coding (614/1476 nt) yeaI → putative membrane‑anchored diguanylate cyclase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 1,983,761 C→T E437K (GAA→AAA)  araG ← L‑arabinose ABC transporter ATPase
RA 1,988,339 C→T Q40* (CAG→TAG)  yecR → lipoprotein, function unknown
RA 2,044,638 C→A intergenic (+14/‑300) asnT → / → yeeJ tRNA‑Asn/putative adhesin
RA 2,071,321 A→G intergenic (+110/‑218) yeeP → / → flu pseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,076,841 G→A A9T (GCC→ACC)  yeeT → CP4‑44 prophage; uncharacterized protein
RA 2,090,860 G→A A223A (GCG→GCA hisG → ATP phosphoribosyltransferase
RA 2,165,590 Δ1 bp intergenic (+69/‑78) yegP → / → yegQ UPF0339 family protein/putative peptidase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,183,656 T→A intergenic (‑5/+60) yegX ← / ← thiD putative family 25 glycosyl hydrolase/hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase
RA 2,191,432 C→T G72S (GGC→AGC)  yehC ← putative periplasmic pilin chaperone
RA 2,210,811 C→T pseudogene (1712/2001 nt) yehQ → pseudogene
RA 2,239,199 T→C N31D (AAC→GAC)  mglA ← methyl‑galactoside ABC transporter ATPase
RA 2,248,192 A→T L114Q (CTG→CAG)  lysP ← lysine transporter
RA 2,278,298 C→T intergenic (‑61/+272) yejG ← / ← bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
RA 2,299,499 A→G L27S (TTA→TCA)  napH ← ferredoxin‑type protein
RA 2,306,399 C→T W119* (TGG→TAG)  mqo ← malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,307,386 A→T D410E (GAT→GAA yojI ← microcin J25 efflux ABC transporter permease/ATPase
RA 2,308,182 A→T L145Q (CTG→CAG)  yojI ← microcin J25 efflux ABC transporter permease/ATPase
RA 2,436,937 A→T D305E (GAT→GAA pdxB ← erythronate‑4‑phosphate dehydrogenase
RA 2,457,953 T→C K403E (AAA→GAA)  fadJ ← enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,461,483 G→T A60S (GCA→TCA)  fadL → long‑chain fatty acid outer membrane transporter
RA 2,469,656 (T)7→8 coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,521,967 C→A A171S (GCG→TCG)  xapR ← transcriptional activator of xapAB
RA 2,563,518 (A)5→6 intergenic (+401/‑74) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,564,525 G→A M1M (ATG→ATA) † yffS → CPZ‑55 prophage; uncharacterized protein
RA 2,600,288 C→T S128L (TCG→TTG)  gcvR → transcriptional repressor, regulatory protein accessory to GcvA
RA 2,715,439 T→C L6S (TTA→TCA)  eamB → cysteine and O‑acetylserine exporter
RA 2,720,543 C→A R197R (CGC→CGA pka → protein lysine acetyltransferase
RA 2,759,132 A→G I50V (ATT→GTT)  yfjI → CP4‑57 prophage; uncharacterized protein
RA 2,759,497 T→C C171C (TGT→TGC yfjI → CP4‑57 prophage; uncharacterized protein
RA 2,782,076 T→C V217V (GTA→GTG ypjA ← adhesin‑like autotransporter
RA 2,810,756 G→A intergenic (+77/‑14) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 2,852,356 T→A L74Q (CTG→CAG)  hypD → hydrogenase maturation protein
RA 2,887,352 T→A intergenic (‑133/+226) ygcB ← / ← cysH Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
RA 2,892,993 G→A E113K (GAA→AAA)  ygcN → putative oxidoreductase
RA 2,964,868 T→C K96K (AAA→AAG thyA ← thymidylate synthetase
RA 2,971,955 G→A R120H (CGC→CAC)  tas → putative NADP(H)‑dependent aldo‑keto reductase
RA 2,987,655 Δ1 bp coding (120/633 nt) yqeH → putative LuxR family transcriptional regulator
RA 2,992,747 A→C E218D (GAA→GAC ygeH → putative transcriptional regulator
RA 2,998,129 C→T pseudogene (700/1137 nt) ygeQ ← pseudogene, glycosyl hydrolase family 15, part of T3SS PAI ETT2 remnant
RA 3,017,118 C→A R353R (CGC→CGA ygfK → putative Fe‑S subunit oxidoreductase subunit
RA 3,070,609 T→C K212K (AAA→AAG fbaA ← fructose‑bisphosphate aldolase, class II
RA 3,118,566 C→T A183T (GCG→ACG)  sslE ← putative secreted and surface‑associated lipoprotein mucinase
RA 3,190,398 T→G M173R (ATG→AGG)  yqiH → putative periplasmic pilin chaperone
RA 3,239,779 C→T intergenic (+234/‑165) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,244,928 T→C intergenic (‑187/‑296) uxaC ← / → exuT uronate isomerase/hexuronate transporter
RA 3,379,768 +C coding (230/1128 nt) zapE ← divisome ATPase
RA 3,452,057 A→T I77N (ATC→AAC)  rplD ← 50S ribosomal subunit protein L4
RA 3,469,544 G→C P104A (CCT→GCT)  chiA ← periplasmic endochitinase
RA 3,542,354 Δ1 bp coding (1948/2322 nt) feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,608,471 T→A L674Q (CTG→CAG)  zntA → zinc, cobalt and lead efflux system
RA 3,622,796 T→G L1202R (CTG→CGG)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 3,638,525 A→G E295G (GAA→GGA)  pitA → phosphate transporter, low‑affinity; tellurite importer
RA 3,639,576 (A)6→5 coding (145/336 nt) uspB ← universal stress (ethanol tolerance) protein B
RA 3,648,042 T→C intergenic (‑254/‑486) dinQ ← / → arsR UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor
RA 3,686,929 G→T Q749K (CAG→AAG)  bcsC ← cellulose synthase subunit
RA 3,727,880 T→C intergenic (‑132/+37) yiaB ← / ← xylB YiaAB family inner membrane protein/xylulokinase
RA 3,783,402 A→G V87A (GTC→GCC)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)
RA 3,828,936 T→C intergenic (‑271/‑9) gltS ← / → xanP glutamate transporter/xanthine permease
RA 3,853,909 A→G intergenic (+267/‑13) tisB → / → emrD toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
RA 3,859,191 T→C D309G (GAT→GGT)  yidK ← putative transporter
RA 3,910,662 A→T D288E (GAT→GAA pstS ← phosphate ABC transporter periplasmic binding protein
RA 3,945,528 T→C noncoding (1825/2904 nt) rrlC → 23S ribosomal RNA of rrnC operon
RA 3,950,468 G→T intergenic (+48/‑39) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein
RA 3,965,820 G→A A20A (GCG→GCA trxA → thioredoxin 1
RA 3,971,690 A→C Q144P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
RA 3,982,684 (C)8→9 noncoding (79/87 nt) leuT → tRNA‑Leu
RA 4,168,540 T→A intergenic (+93/‑101) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA of rrnB operon
RA 4,235,689 +A intergenic (+282/‑217) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
MC JC 4,242,942 Δ66 bp coding (510‑575/891 nt) malG ← maltose transporter subunit
RA 4,251,287 T→C pseudogene (327/1323 nt) yjbI → pseudogene, SopA‑related, pentapeptide repeats‑containing
RA 4,281,967 T→C Q370R (CAG→CGG)  yjcF ← pentapeptide repeats protein
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,479,551 A→G intergenic (‑14/‑179) yjgM ← / → yjgN GNAT family putative N‑acetyltransferase/DUF898 family inner membrane protein
RA 4,490,055 T→G E3D (GAA→GAC yjgR ← DUF853 family protein with NTPase fold
RA 4,551,633 A→G intergenic (‑337/‑3) gntP ← / → uxuA fructuronate transporter/mannonate hydrolase
RA 4,555,796 (T)5→4 coding (525/831 nt) yjiC ← uncharacterized protein
RA 4,572,919 A→T pseudogene (506/1503 nt) yjiT → pseudogene
RA 4,615,859 T→G T403P (ACC→CCC)  yjjI ← DUF3029 family protein, putative glycine radical enzyme
RA 4,616,491 T→C D192G (GAT→GGT)  yjjI ← DUF3029 family protein, putative glycine radical enzyme
RA 4,631,700 T→C L323P (CTT→CCT)  slt → lytic murein transglycosylase, soluble

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423710–3424234 3424590–3424238 5–881 23 [20] [22] 23 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =9 (0.170)31 (0.690) 27/166 0.9 80.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 8 (0.180)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078059 (0.170)44 (0.980) 39/166 0.2 85.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 8 (0.180)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)62 (1.210) 46/190 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein