Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	100%	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	99 [0]	[0] 99	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	83 (1.070)	61/170	NT	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGT  >  NC_000913/1299404‑1299596                                                                                               |                                                                                                   catGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTCAataat                                                                                               <  2:1565963‑M1/100‑6 (MQ=255) catGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataat                                                                                               >  2:685454‑M1/1‑95 (MQ=255) catGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataat                                                                                               <  2:1077011‑M1/100‑6 (MQ=255)  atGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                              >  2:1438244‑M1/1‑94 (MQ=255)    gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                                                                            >  2:1916884‑M1/1‑92 (MQ=255)    gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                                                                            >  2:689442‑M1/1‑92 (MQ=255)        ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                        >  1:1232915‑M1/1‑88 (MQ=255)        ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                        <  1:323589‑M1/100‑13 (MQ=255)         tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGGGGAAAGTGCTAAataataatcaatt                                                                                       >  2:698318‑M1/1‑87 (MQ=255)         tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                       >  2:1285684‑M1/1‑87 (MQ=255)         tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                       >  2:366356‑M1/1‑87 (MQ=255)          ttCGCCACATCATAATCCTGGACTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattg                                                                                      >  1:451840‑M1/1‑86 (MQ=255)            cGCCACATCATAATCCTGGGCTTACTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatgaattgtt                                                                                    >  2:2074001‑M1/1‑84 (MQ=255)             gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                                                                   <  1:1260073‑M1/100‑18 (MQ=255)             gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCATTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                                                                   >  2:231744‑M1/1‑83 (MQ=255)             gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTACTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtta                                                                                   >  2:273629‑M1/1‑83 (MQ=255)               cacaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                 >  2:461585‑M1/1‑81 (MQ=255)                     atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattg                                                                           >  1:1007793‑M1/1‑75 (MQ=255)                      tAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                                                                          <  2:182433‑M1/100‑27 (MQ=255)                        aTCCTTGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgc                                                                        <  2:5273‑M1/100‑29 (MQ=255)                        aTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgc                                                                        >  1:345732‑M1/1‑72 (MQ=255)                          ccTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcat                                                                      >  2:1398564‑M1/1‑70 (MQ=255)                          ccTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcat                                                                      >  1:1479091‑M1/1‑70 (MQ=255)                             gggCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttc                                                                   <  1:618921‑M1/100‑34 (MQ=255)                                cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcact                                                                <  1:1916767‑M1/100‑37 (MQ=255)                                cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcact                                                                <  1:2171321‑M1/100‑37 (MQ=255)                                 ttGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                               <  1:473393‑M1/100‑38 (MQ=255)                                  tGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                              >  1:1845811‑M1/1‑62 (MQ=255)                                    cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                            >  1:1106817‑M1/1‑60 (MQ=255)                                    cTGAAGAATAATTGAAATGATATTATTAATTACACTGACTTTAGTAGAGAAAAGTGCTAAataataatcaattgttaaattattatgcatttaactac                                                              >  2:2126562‑M1/1‑60 (MQ=255)                                     tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                           <  1:1285690‑M1/100‑42 (MQ=255)                                     tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                           >  2:454716‑M1/1‑59 (MQ=255)                                      gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgga                                                          >  1:1132747‑M1/1‑58 (MQ=255)                                         gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                       >  2:1160237‑M1/1‑55 (MQ=255)                                         gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                       >  2:305466‑M1/1‑55 (MQ=255)                                         gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                       >  1:1238102‑M1/1‑55 (MQ=255)                                           ataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                     <  2:2070732‑M1/100‑48 (MQ=255)                                           ataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                     >  1:2146847‑M1/1‑53 (MQ=255)                                           ataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                     >  1:2075886‑M1/1‑53 (MQ=255)                                            taatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgta                                                    <  2:773594‑M1/100‑49 (MQ=255)                                            cgatctaaatgatatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgta                                                    <  2:1925886‑M1/94‑49 (MQ=255)                                                 gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcag                                               <  2:1132744‑M1/100‑54 (MQ=255)                                                  aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaagatcg                                              >  1:1925899‑M1/1‑46 (MQ=255)                                                     tGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                           <  2:1773986‑M1/100‑58 (MQ=255)                                                       atatTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaaga                                         <  2:1479081‑M1/100‑60 (MQ=255)                                                        tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTTAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                        <  2:181742‑M1/100‑61 (MQ=255)                                                        tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                        >  1:255912‑M1/1‑40 (MQ=255)                                                        tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                        >  1:1629113‑M1/1‑40 (MQ=255)                                                         attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                       >  2:1841732‑M1/1‑39 (MQ=255)                                                          ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                      >  1:504615‑M1/1‑38 (MQ=255)                                                          ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                      <  1:860953‑M1/100‑63 (MQ=255)                                                           tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                     <  2:1918606‑M1/100‑64 (MQ=255)                                                           tattaATTCCACTGCCTTTGGAAAAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                     <  2:1797367‑M1/100‑64 (MQ=255)                                                            attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagac                                    <  1:2183799‑M1/100‑65 (MQ=255)                                                            attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagac                                    <  1:2011338‑M1/100‑65 (MQ=255)                                                               aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                 <  1:1764527‑M1/100‑68 (MQ=255)                                                                aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                <  1:254162‑M1/100‑69 (MQ=255)                                                                aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                <  1:1565286‑M1/100‑69 (MQ=255)                                                                 ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgct                               <  1:685454‑M1/100‑70 (MQ=255)                                                                 ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgct                               >  2:840003‑M1/1‑31 (MQ=255)                                                                  tCCACTGCTTTTAGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctt                              <  2:334712‑M1/100‑71 (MQ=255)                                                                  tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctt                              <  2:1970993‑M1/100‑71 (MQ=255)                                                                   ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                             <  2:1606478‑M1/100‑72 (MQ=255)                                                                   ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                             >  1:484199‑M1/1‑29 (MQ=255)                                                                     aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                           <  2:1058592‑M1/100‑74 (MQ=255)                                                                      cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcc                          >  2:152195‑M1/1‑26 (MQ=255)                                                                       tgtctttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcca                         <  1:630897‑M1/97‑76 (MQ=255)                                                                       tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcca                         >  1:1984546‑M1/1‑25 (MQ=255)                                                                        gCCTTTGTTAGAGGAAAGTACTAAataataatcaattgttaaattattatgcatttaactactggaactgtaatcagaaaagatagacatgcttagcca                         >  2:495698‑M1/1‑24 (MQ=255)                                                                        gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaa                        >  2:335611‑M1/1‑24 (MQ=255)                                                                          cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                      <  2:838354‑M1/100‑79 (MQ=255)                                                                           tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatct                     >  1:2040259‑M1/1‑21 (MQ=255)                                                                            ttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctc                    <  2:251332‑M1/100‑81 (MQ=255)                                                                                 agagTAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                 <  2:1622014‑M1/98‑84 (MQ=255)                                                                                 agagGAAAGTGTTAAataataatcaatagttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttatccaatctctattt               >  2:829553‑M1/1‑15 (MQ=255)                                                                                 agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt               <  1:2075041‑M1/100‑86 (MQ=255)                                                                                 agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt               >  2:1828403‑M1/1‑15 (MQ=255)                                                                                  gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttg              >  1:2203310‑M1/1‑14 (MQ=255)                                                                                   agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtactcagaaaagatagacaagcttagccaatctctatgtta             >  1:795881‑M1/1‑13 (MQ=255)                                                                                   agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcatagccaatctctatttg              <  2:2158917‑M1/99‑87 (MQ=255)                                                                                    ggAAAGTGCTAAataataatcacttgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat            >  2:1301822‑M1/1‑12 (MQ=255)                                                                                    ggAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat            <  2:2040245‑M1/100‑89 (MQ=255)                                                                                     gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat            <  1:335610‑M1/99‑89 (MQ=255)                                                                                      aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattg          <  2:246212‑M1/100‑91 (MQ=255)                                                                                      aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattg          <  1:472859‑M1/100‑91 (MQ=255)                                                                                      aaaGTGCTAAataataatcaattgttaaatgattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaagctctatttgattg          <  2:1479972‑M1/100‑91 (MQ=255)                                                                                         gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat       <  2:1530361‑M1/100‑94 (MQ=255)                                                                                         gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat       <  1:1373369‑M1/100‑94 (MQ=255)                                                                                         gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat       >  1:1442011‑M1/1‑7 (MQ=255)                                                                                          tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt      >  2:592311‑M1/1‑6 (MQ=255)                                                                                            cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcataaaagatagacatgcttagccaatctctatttgattgaattga    <  2:1048202‑M1/100‑97 (MQ=255)                                                                                            cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattga    >  1:64600‑M1/1‑4 (MQ=255)                                                                                            cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat       >  1:424218‑M1/1‑4 (MQ=255)                                                                                             tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaa   >  2:958319‑M1/1‑3 (MQ=255)                                                                                             tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaa   >  2:312434‑M1/1‑3 (MQ=255)                                                                                             tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaa   >  2:312433‑M1/1‑3 (MQ=255)                                                                                              aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa  >  1:1630073‑M1/1‑2 (MQ=255)                                                                                              aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa  <  2:732522‑M1/100‑99 (MQ=255)                                                                                              aaataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa  >  2:591885‑M1/1‑2 (MQ=255)                                                                                               |                                                                                                   CATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGT  >  NC_000913/1299404‑1299596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑