breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009134,296,0600C→T19.7% 29.7 ‑3.4 ‑0.071intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_000913380,0121.→G13.5% 19.8 ‑3.2 ‑0.337intergenic (‑131/+57)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
*NC_0009133,781,1200T→C28.6% 15.0 ‑7.5 0.036intergenic (+103/‑95)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
*NC_0009133,781,1400A→G38.7% 12.9 ‑5.7 0.031intergenic (+123/‑75)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
*NC_0009133,781,1520A→G30.3% 12.5 ‑5.0 ‑0.033intergenic (+135/‑63)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
*NC_0009133,424,8560T→G100.0% 1.0 1noncoding (1928/2904 nt)rrlD23S ribosomal RNA of rrnD operon
*NC_0009133,915,6450G→T100.0% 0.7 1H110N (CAC→AAC) atpCF1 sector of membrane‑bound ATP synthase, epsilon subunit
*NC_0009133,915,9200G→T100.0% 0.7 1S18Y (TCT→TAT) atpCF1 sector of membrane‑bound ATP synthase, epsilon subunit
*NC_0009133,366,5530A→C100.0% 0.6 1F56V (TTT→GTT) insH1IS5 transposase and trans‑activator
*NC_0009133,915,7430A→G100.0% 0.6 1V77A (GTG→GCG) atpCF1 sector of membrane‑bound ATP synthase, epsilon subunit
*NC_000913279,7430A→G100.0% 0.5 1Y45H (TAC→CAC) insAIS1 repressor TnpA
*NC_000913270,7350G→C100.0% 0.4 1G45R (GGC→CGC) insI1IS30 transposase
*NC_000913270,7380G→C100.0% 0.4 1G46R (GGC→CGC) insI1IS30 transposase
*NC_0009133,470,3690T→A100.0% 0.0 1T321S (ACT→TCT) tufAtranslation elongation factor EF‑Tu 1
*NC_0009133,427,1480A→G100.0% ‑0.2 1noncoding (5/77 nt)ileUtRNA‑Ile
*NC_0009133,427,1640A→C100.0% ‑0.2 1intergenic (‑12/+57)ileU/rrsDtRNA‑Ile/16S ribosomal RNA of rrnD operon
*NC_0009133,427,1710A→T100.0% ‑0.2 1intergenic (‑19/+50)ileU/rrsDtRNA‑Ile/16S ribosomal RNA of rrnD operon
*NC_0009133,427,1750T→A100.0% ‑0.2 1intergenic (‑23/+46)ileU/rrsDtRNA‑Ile/16S ribosomal RNA of rrnD operon
*NC_0009133,427,1790C→A100.0% ‑0.2 1intergenic (‑27/+42)ileU/rrsDtRNA‑Ile/16S ribosomal RNA of rrnD operon
*NC_0009133,427,1830C→A100.0% ‑0.2 1intergenic (‑31/+38)ileU/rrsDtRNA‑Ile/16S ribosomal RNA of rrnD operon
*NC_0009133,427,1860T→C100.0% ‑0.2 1intergenic (‑34/+35)ileU/rrsDtRNA‑Ile/16S ribosomal RNA of rrnD operon
*NC_0009133,427,1870C→T100.0% ‑0.2 1intergenic (‑35/+34)ileU/rrsDtRNA‑Ile/16S ribosomal RNA of rrnD operon
*NC_0009133,424,9840G→A100.0% ‑0.5 1noncoding (1800/2904 nt)rrlD23S ribosomal RNA of rrnD operon
*NC_0009133,424,9850C→A100.0% ‑0.5 1noncoding (1799/2904 nt)rrlD23S ribosomal RNA of rrnD operon
*NC_0009133,424,9870C→A100.0% ‑0.5 1noncoding (1797/2904 nt)rrlD23S ribosomal RNA of rrnD operon
*NC_0009133,424,9890G→A100.0% ‑0.5 1noncoding (1795/2904 nt)rrlD23S ribosomal RNA of rrnD operon

Marginal mixed read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_000913556,6400T→A11.7% 12.6 ‑0.4 ‑0.560intergenic (‑87/+21)ybcI/ybcJDUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
*NC_000913556,6410G→T13.6% 12.6 0.0 0.059intergenic (‑88/+20)ybcI/ybcJDUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
*NC_000913556,6420A→C13.8% 16.2 0.0 ‑0.059intergenic (‑89/+19)ybcI/ybcJDUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
*NC_000913556,6430T→A12.5% 12.8 ‑0.4 ‑0.258intergenic (‑90/+18)ybcI/ybcJDUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
*NC_0009131,297,2670A→G12.7% 12.6 ‑0.4 ‑0.555V285V (GTT→GTCadhEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
*NC_0009131,297,2680A→T12.7% 13.2 ‑0.4 ‑0.555V285D (GTT→GAT) adhEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
*NC_0009131,297,2690C→T13.0% 12.3 ‑0.4 ‑0.454V285I (GTT→ATT) adhEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
*NC_0009133,181,6000A→G11.5% 11.6 0.0 ‑0.162intergenic (+19/+19)ygiC/ygiDATP‑Grasp family ATPase/4,5‑ DOPA‑extradiol‑dioxygenase
*NC_0009133,181,6010C→A11.3% 13.8 0.0 ‑0.262intergenic (+20/+18)ygiC/ygiDATP‑Grasp family ATPase/4,5‑ DOPA‑extradiol‑dioxygenase
*NC_0009133,181,6020T→G11.5% 15.1 0.0 ‑0.361intergenic (+21/+17)ygiC/ygiDATP‑Grasp family ATPase/4,5‑ DOPA‑extradiol‑dioxygenase
*NC_0009133,181,6030C→T11.7% 11.5 0.0 ‑0.361intergenic (+22/+16)ygiC/ygiDATP‑Grasp family ATPase/4,5‑ DOPA‑extradiol‑dioxygenase

Marginal new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 315651NA (NA)6 (0.120) 5/402 7.7 NA noncoding (423/1255 nt) IS3 repeat region
?NC_000913 = 315703 NA (NA)noncoding (475/1255 nt) IS3 repeat region
* ? NC_000913 1207790 =42 (0.780)5 (0.100) 5/386 7.5 11.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 35 (0.710)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 270589463 (1.180)4 (0.080) 4/400 8.3 6.3% coding (1231/1800 nt) lepA back‑translocating elongation factor EF4, GTPase
?NC_000913 = 2705947 58 (1.130)coding (1178/1800 nt) lepA back‑translocating elongation factor EF4, GTPase
* ? NC_000913 = 341418563 (1.180)4 (0.080) 4/406 8.4 6.1% coding (322/1158 nt) acrE cytoplasmic membrane lipoprotein
?NC_000913 = 3414221 62 (1.190)coding (358/1158 nt) acrE cytoplasmic membrane lipoprotein
* ? NC_000913 224575 =1 (0.020)3 (0.060) 3/404 9.1 75.6% noncoding (805/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 = 2730305 NA (NA)noncoding (853/1542 nt) rrsG 16S ribosomal RNA of rrnG operon
* ? NC_000913 = 200646045 (0.840)3 (0.060) 3/406 9.1 6.3% coding (305/1488 nt) amyA cytoplasmic alpha‑amylase
?NC_000913 = 2006465 45 (0.860)coding (310/1488 nt) amyA cytoplasmic alpha‑amylase
* ? NC_000913 2647212 =62 (1.160)3 (0.060) 3/412 9.2 4.8% coding (114/2313 nt) pbpC penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
?NC_000913 2647262 = 57 (1.080)coding (64/2313 nt) pbpC penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
* ? NC_000913 = 2730312NA (NA)3 (0.060) 3/404 9.1 NA noncoding (846/1542 nt) rrsG 16S ribosomal RNA of rrnG operon
?NC_000913 = 2730346 NA (NA)noncoding (812/1542 nt) rrsG 16S ribosomal RNA of rrnG operon
* ? NC_000913 4080093 =52 (0.970)3 (0.060) 3/404 9.1 5.6% noncoding (74/98 nt) REP297 (repetitive extragenic palindromic) element; contains 2 REP sequences REP297 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913 4080118 = 51 (0.990)intergenic (+149/+181) yiiF/fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
* ? NC_000913 4142066 =46 (0.860)3 (0.060) 3/400 9.0 6.2% coding (156/2502 nt) frwA putative PTS enzyme: Hpr, enzyme I and II components
?NC_000913 4142096 = 47 (0.920)coding (126/2502 nt) frwA putative PTS enzyme: Hpr, enzyme I and II components