breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009132,590,7710C→G16.7% 10.7 ‑1.1 ‑0.630intergenic (+63/+36)acrD/ypfMaminoglycoside/multidrug efflux system/stress‑induced small enterobacterial protein
*NC_000913867,8220T→C16.2% 10.5 ‑1.0 ‑0.937V101V (GTT→GTCgsiAglutathione ABC transporter ATPase
*NC_0009133,875,5210T→C20.5% 10.5 ‑4.2 ‑0.040A28A (GCT→GCCyidXputative lipoprotein
*NC_0009133,762,4660G→C100.0% 1.5 1G95A (GGC→GCC) rhsARhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
*NC_0009131,433,8730C→T100.0% 1.0 2intergenic (‑199/+118)pinR/ynaERac prophage; putative site‑specific recombinase/cold shock protein, Rac prophage
*NC_0009133,942,2380A→C100.0% 1.0 1noncoding (431/1542 nt)rrsC16S ribosomal RNA of rrnC operon
*NC_0009131,468,1120T→G100.0% 0.5 1T239P (ACG→CCG) insD1IS2 transposase TnpB
*NC_0009131,571,0690G→T100.0% ‑0.0 1A326E (GCA→GAA) gadBglutamate decarboxylase B, PLP‑dependent

Marginal mixed read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_000913774,6230A→T15.8% 10.4 0.0 ‑0.738intergenic (+21/‑129)ybgE/ybgCputative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase
*NC_000913774,6330G→T14.3% 12.6 0.0 ‑0.742intergenic (+31/‑119)ybgE/ybgCputative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase
*NC_000913774,6340C→G14.3% 13.4 0.0 ‑0.542intergenic (+32/‑118)ybgE/ybgCputative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase
*NC_000913774,6350T→G14.6% 12.5 0.0 ‑0.241intergenic (+33/‑117)ybgE/ybgCputative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase
*NC_000913774,6360C→A17.1% 12.5 0.0 ‑0.041intergenic (+34/‑116)ybgE/ybgCputative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase
*NC_000913774,6370C→G14.6% 13.6 0.0 ‑0.341intergenic (+35/‑115)ybgE/ybgCputative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase
*NC_000913774,6380A→C14.6% 11.9 0.0 ‑0.341intergenic (+36/‑114)ybgE/ybgCputative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase

Marginal new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 437107 =NA (NA)3 (0.070) 3/416 7.0 6.4% coding (519/519 nt) pgpA phosphatidylglycerophosphatase A
?NC_000913 933756 = 44 (1.070)coding (533/3990 nt) ftsK DNA translocase at septal ring sorting daughter chromsomes
* ? NC_000913 1888472 =53 (1.280)3 (0.080) 3/396 6.8 6.2% coding (1275/1686 nt) fadD acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
?NC_000913 1888616 = 41 (1.050)coding (1131/1686 nt) fadD acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
* ? NC_000913 3040949 =32 (0.780)3 (0.080) 3/400 6.8 9.3% coding (122/267 nt) sdhE flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
?NC_000913 3040995 = 28 (0.710)coding (76/267 nt) sdhE flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
* ? NC_000913 3593269 =39 (0.940)3 (0.070) 3/408 6.9 7.2% coding (169/714 nt) livF branched‑chain amino acid ABC transporter ATPase
?NC_000913 3593337 = 39 (0.970)coding (101/714 nt) livF branched‑chain amino acid ABC transporter ATPase