breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009131,666,4660T→G18.8% 13.8 ‑1.0 ‑0.532Y384* (TAT→TAGclcBH(+)/Cl(‑) exchange transporter
*NC_0009131,666,4700C→A20.0% 13.3 ‑1.0 ‑0.130L386I (CTA→ATA) clcBH(+)/Cl(‑) exchange transporter
*NC_0009133,250,9890G→C10.3% 12.6 ‑1.0 ‑0.358intergenic (+35/‑35)yqjF/yqjGputative quinol oxidase subunit/putative S‑transferase
*NC_0009131,666,4680A→T19.4% 11.7 ‑1.0 ‑0.331Q385L (CAG→CTG) clcBH(+)/Cl(‑) exchange transporter
*NC_0009131,571,5950G→T100.0% 0.9 1P151T (CCA→ACA) gadBglutamate decarboxylase B, PLP‑dependent
*NC_0009131,433,8620C→A100.0% 0.8 1intergenic (‑188/+129)pinR/ynaERac prophage; putative site‑specific recombinase/cold shock protein, Rac prophage
*NC_000913566,9530G→C100.0% 0.7 1E38Q (GAA→CAA) insE1IS3 transposase A
*NC_0009134,035,9370T→G100.0% 0.6 1noncoding (407/1542 nt)rrsA16S ribosomal RNA of rrnA operon
*NC_0009133,622,0940A→C100.0% 0.5 1H968P (CAT→CCT) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
*NC_0009133,619,4660T→C100.0% 0.4 1L92P (CTC→CCC) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
*NC_0009133,943,7680T→C100.0% 0.4 1noncoding (65/2904 nt)rrlC23S ribosomal RNA of rrnC operon
*NC_0009133,763,8790A→G100.0% 0.0 2E566G (GAG→GGG) rhsARhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
*NC_0009133,763,8820A→G100.0% 0.0 2E567G (GAA→GGA) rhsARhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
*NC_0009134,038,0300G→T100.0% ‑0.0 1noncoding (512/2905 nt)rrlA23S ribosomal RNA of rrnA operon

Marginal mixed read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_000913458,7380G→A19.4% 11.0 ‑0.9 ‑0.136intergenic (+38/‑150)clpX/lonATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
*NC_000913458,7420T→A19.4% 13.7 ‑0.9 ‑0.137intergenic (+42/‑146)clpX/lonATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
*NC_000913458,7430T→A19.4% 13.3 ‑0.9 ‑0.037intergenic (+43/‑145)clpX/lonATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
*NC_000913458,7440T→A18.9% 10.8 ‑0.9 0.037intergenic (+44/‑144)clpX/lonATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
*NC_000913458,7480T→C18.4% 11.4 ‑0.9 ‑0.238intergenic (+48/‑140)clpX/lonATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
*NC_0009131,594,1241.→C12.9% 13.9 0.0 ‑0.331pseudogene (2/1461 nt)yneOpseudogene, AidA homolog
*NC_0009132,663,4660G→T11.8% 11.5 ‑0.4 ‑0.068V9L (GTG→TTG) suhBinositol monophosphatase
*NC_0009132,663,4740A→T10.4% 11.3 0.0 ‑0.167A11A (GCA→GCTsuhBinositol monophosphatase
*NC_0009132,663,4820A→C10.1% 13.8 0.0 ‑0.170K14T (AAG→ACG) suhBinositol monophosphatase
*NC_0009132,673,7840C→A10.0% 11.6 ‑0.4 ‑0.261intergenic (+16/+32)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
*NC_0009132,673,7900G→T12.5% 12.1 ‑0.3 ‑0.064intergenic (+22/+26)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
*NC_0009132,673,7910A→C10.9% 12.8 0.0 ‑0.164intergenic (+23/+25)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
*NC_0009133,946,6200A→C25.4% 40.0 ‑0.3 ‑0.159intergenic (+13/‑80)rrlC/rrfC23S ribosomal RNA of rrnC operon/5S ribosomal RNA of rrnC operon
*NC_0009134,060,2610G→T17.5% 12.8 ‑0.9 ‑0.040intergenic (+31/‑186)typA/yihLGTP‑binding protein/putative DNA‑binding transcriptional regulator
*NC_0009134,060,2640A→C15.0% 13.6 ‑0.4 ‑0.640intergenic (+34/‑183)typA/yihLGTP‑binding protein/putative DNA‑binding transcriptional regulator
*NC_0009134,296,0600C→T23.3% 12.4 0.0 ‑0.033intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Marginal new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2611254 =43 (1.140)4 (0.110) 4/404 4.6 7.7% coding (549/759 nt) hyfI hydrogenase 4, Fe‑S subunit
?NC_000913 2611305 = 55 (1.510)coding (600/759 nt) hyfI hydrogenase 4, Fe‑S subunit
* ? NC_000913 351579 =29 (0.770)3 (0.080) 3/402 5.1 10.2% coding (365/1452 nt) prpD 2‑methylcitrate dehydratase
?NC_000913 351632 = 25 (0.690)coding (418/1452 nt) prpD 2‑methylcitrate dehydratase
* ? NC_000913 424447 =40 (1.060)3 (0.080) 3/402 5.1 7.8% coding (472/582 nt) acpH acyl carrier protein (ACP) phosphodiesterase; ACP hydrolyase
?NC_000913 424497 = 32 (0.890)coding (422/582 nt) acpH acyl carrier protein (ACP) phosphodiesterase; ACP hydrolyase
* ? NC_000913 643269 =28 (0.740)3 (0.080) 3/404 5.1 11.5% coding (1182/1239 nt) ybdR putative Zn‑dependent NAD(P)‑binding oxidoreductase
?NC_000913 643379 = 19 (0.520)intergenic (+53/+178) ybdR/rnk putative Zn‑dependent NAD(P)‑binding oxidoreductase/regulator of nucleoside diphosphate kinase
* ? NC_000913 1859579 =20 (0.530)3 (0.080) 3/404 5.1 11.9% coding (651/1380 nt) ydjK putative MFS sugar transporter, membrane protein
?NC_000913 1859626 = 25 (0.690)coding (604/1380 nt) ydjK putative MFS sugar transporter, membrane protein
* ? NC_000913 2243148 =37 (0.980)3 (0.080) 3/402 5.1 8.2% coding (505/669 nt) folE GTP cyclohydrolase I
?NC_000913 2243219 = 32 (0.890)coding (434/669 nt) folE GTP cyclohydrolase I
* ? NC_000913 3247830 =62 (1.650)3 (0.080) 3/404 5.1 4.7% coding (58/663 nt) yqjA general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
?NC_000913 3247851 = 62 (1.710)coding (79/663 nt) yqjA general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
* ? NC_000913 3390623 =26 (0.690)3 (0.080) 3/406 5.2 10.1% coding (1406/1446 nt) tldD putative peptidase
?NC_000913 3390643 = 28 (0.770)coding (1386/1446 nt) tldD putative peptidase
* ? NC_000913 = 387285831 (0.820)4 (0.110) 3/412 5.2 11.7% coding (141/1149 nt) dgoD D‑galactonate dehydratase
?NC_000913 = 3872904 30 (0.810)coding (95/1149 nt) dgoD D‑galactonate dehydratase