Predicted mutation
evidence seq id position mutation annotation gene description
MC JC DH1 163,135 Δ95,041 bp hokAyhhJ 83 genes

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ DH1 163135 258175 95041 602 [3] [3] 605 hokA–yhhJ 83 genes
hokA, cspA, yiaG, yiaF, ghrB, yiaD, bisC, yiaC, tag, yhjY, yhjX, eptB, ECDH1_RS00855, ECDH1_RS00860, dppA, ECDH1_RS00870, dppB, dppC, dppD, dppF, yhjV, ECDH1_RS00900, ldrD, bcsG, bcsF, bcsE, yhjR, ECDH1_RS00930, bcsA, bcsB, bcsZ, bcsC, yhjK, dctA, yhjJ, kdgK, ECDH1_RS00975, yhjH, yhjG, yhjE, yhjD, yhjC, yhjB, treF, yhjA, gadA, gadX, gadW, mdtF, mdtE, ECDH1_RS01045, gadE, hdeD, hdeA, hdeB, yhiD, dctR, slp, slp, insH1, ECDH1_RS01095, arsC, arsB, arsR, ECDH1_RS01115, ECDH1_RS01120, gor, rlmJ, prlC, rsmJ, tppB, uspA, uspB, pitA, yhiN, yhiM, ECDH1_RS01175, ECDH1_RS01180, ECDH1_RS01185, yhiJ, yhiI, rbbA, yhhJ

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 1631343 (0.010)590 (1.190) 252/298 0.0 99.5% intergenic (‑51/‑29) insJ/hokA transposase/protein HokA
?DH1 258176 = 3 (0.010)intergenic (+92/+73) yhhJ/yhhI inner membrane transport permease YhhJ/H repeat‑associated protein yhhI

Only 100 of 605 total aligned reads displayed.

GCGCATTTTTCACCATTGCGCTGCTGCGATTCAGGAAGACGATTGGGACAATGGCGTAAATACAATTGCCCGATGCGTAAGCTTATAGGGACTACGCATCGGTGCAATTTATTGAATTTTAGTTGTTTTTAGGCCGGATAAGGCGTTTATGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAA  >  DH1/258026‑258326
                                                                                                                                                      |                                                                                                                                                      
accaaaacgtgccgagatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactg                                                                                                                                                        >  4:1633167‑M2/151‑151 (MQ=255)
     aacgtgccgagatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactgaactg                                                                                                                                                   >  6:1201312‑M2/146‑151 (MQ=255)
          gccgagatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGCcccc                                                                                                                                              >  2:1294378‑M2/141‑151 (MQ=255)
             gagatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCaa                                                                                                                                           <  8:855338‑M2/14‑1 (MQ=255)
             gagatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCa                                                                                                                                            >  6:886186‑M2/138‑150 (MQ=255)
              agatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCaa                                                                                                                                           >  3:1133849‑M2/137‑150 (MQ=255)
               gatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCaaaa                                                                                                                                         <  5:110330‑M2/16‑1 (MQ=255)
                atgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAAc                                                                                                                                        <  8:1960172‑M2/17‑1 (MQ=255)
                 tgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAAcc                                                                                                                                       >  7:1905556‑M2/134‑151 (MQ=255)
                   atcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTg                                                                                                                                     >  4:19099‑M2/132‑151 (MQ=255)
                     cctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTgg                                                                                                                                    >  5:565007‑M2/130‑150 (MQ=255)
                     cctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTgg                                                                                                                                    <  5:424418‑M2/21‑1 (MQ=255)
                        gtaaccatcatcagttgtgaagtagtgattcatgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAg                                                                                                                                <  2:583770‑M2/25‑1 (MQ=255)
                        gtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACACCCAAAACCTGGACAg                                                                                                                                <  6:1399427‑M2/25‑1 (MQ=255)
                         taaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacataatGAACTGACCCCCAAAACCTTGACAGt                                                                                                                               >  7:1113842‑M2/126‑151 (MQ=255)
                            ccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTc                                                                                                                            <  4:946157‑M2/29‑1 (MQ=255)
                             catcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTc                                                                                                                            >  3:247544‑M2/122‑150 (MQ=255)
                              atcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattctatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTc                                                                                                                            >  3:905537‑M2/121‑149 (MQ=255)
                                catcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGtt                                                                                                                        <  1:940114‑M2/33‑1 (MQ=255)
                                 atcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTa                                                                                                                       <  8:1598477‑M2/34‑1 (MQ=255)
                                 atcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTa                                                                                                                       <  1:17983‑M2/34‑1 (MQ=255)
                                    agttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAAcc                                                                                                                    >  7:610325‑M2/115‑151 (MQ=255)
                                       tgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGc                                                                                                                 >  3:1700051‑M2/112‑151 (MQ=255)
                                          gaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCttt                                                                                                              >  5:117146‑M2/109‑151 (MQ=255)
                                          gaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCttt                                                                                                              <  2:1930012‑M2/43‑1 (MQ=255)
                                              tagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTg                                                                                                           >  3:586989‑M2/105‑150 (MQ=255)
                                                gtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGAt                                                                                                         >  2:1203461‑M2/103‑150 (MQ=255)
                                                gtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATa                                                                                                        <  4:366652‑M2/49‑1 (MQ=255)
                                                   attcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTc                                                                                                     >  5:279774‑M2/100‑151 (MQ=255)
                                                   attcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTc                                                                                                     <  7:226502‑M2/52‑1 (MQ=255)
                                                   attcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTc                                                                                                     <  8:505125‑M2/52‑1 (MQ=255)
                                                   attcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgcttgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGATTTTTGATACTc                                                                                                     >  8:1103036‑M2/100‑151 (MQ=255)
                                                     tcaggaattcaaggcgcttttcaatagggtatttttgctttgacatattaggggctattccatttcatcgtcgaacaaaatgggtgcagtacatactGAAGTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCa                                                                                                    <  1:824321‑M2/53‑1 (MQ=255)
                                                         gacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGCCAGTTTCAGTTAACCGGCTTTTTGATACACAGTCCt                                                                                               >  5:1494652‑M2/94‑151 (MQ=255)
                                                         gacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCt                                                                                               <  1:476638‑M2/58‑1 (MQ=255)
                                                         gacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCt                                                                                               >  5:1714803‑M2/94‑151 (MQ=255)
                                                          acttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTg                                                                                              >  7:1383193‑M2/93‑151 (MQ=255)
                                                            ttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGt                                                                                             >  6:1730741‑M2/91‑150 (MQ=255)
                                                             tcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTAt                                                                                           >  4:1502233‑M2/90‑151 (MQ=255)
                                                              caaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTAtt                                                                                          >  7:880443‑M2/89‑151 (MQ=255)
                                                               aaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTc                                                                                         <  4:58106‑M2/64‑1 (MQ=255)
                                                                   cgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAggg                                                                                     <  5:669288‑M2/68‑1 (MQ=255)
                                                                   cgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAggg                                                                                     >  6:302447‑M2/84‑151 (MQ=255)
                                                                      ttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCa                                                                                   >  3:1477429‑M2/81‑150 (MQ=255)
                                                                         tcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCATTTTGATACTCAGTCCTGTATTCAGGCCa                                                                                   >  2:1672252‑M2/78‑147 (MQ=255)
                                                                          caaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGAtt                                                                              >  4:473236‑M2/77‑151 (MQ=255)
                                                                            aaatggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTAc                                                                            <  7:1607701‑M2/77‑1 (MQ=255)
                                                                              agggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACg                                                                           >  2:830364‑M2/73‑150 (MQ=255)
                                                                               gggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGaa                                                                         <  1:1284677‑M2/80‑1 (MQ=255)
                                                                                ggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGaaa                                                                        <  7:338199‑M2/81‑1 (MQ=255)
                                                                                  tattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGc                                                                      >  1:1398303‑M2/69‑151 (MQ=255)
                                                                                           tttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTcccc                                                             >  4:1959756‑M2/60‑151 (MQ=255)
                                                                                            ttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTcccc                                                             <  1:1879530‑M2/92‑1 (MQ=255)
                                                                                            ttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTcccc                                                             <  2:985549‑M2/92‑1 (MQ=255)
                                                                                             tgaaatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacataatGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCACGCTCCCCGc                                                           >  3:1339174‑M2/58‑151 (MQ=255)
                                                                                              gacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGc                                                           >  7:467652‑M2/57‑150 (MQ=255)
                                                                                               acatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGcac                                                         <  1:100068‑M2/96‑2 (MQ=255)
                                                                                                 atattaggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAgg                                                       >  3:37136‑M2/54‑151 (MQ=255)
                                                                                                     taggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACt                                                    <  8:801064‑M2/101‑1 (MQ=255)
                                                                                                     taggggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTg                                                   >  6:1061610‑M2/50‑151 (MQ=255)
                                                                                                      agtggctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGGTCCCCGCAAGGACTGa                                                  <  2:1382158‑M2/103‑1 (MQ=255)
                                                                                                           ctattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCCAGGACTGACGcc                                              >  4:115595‑M2/44‑150 (MQ=255)
                                                                                                            tattccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGcc                                              >  4:1340670‑M2/43‑149 (MQ=255)
                                                                                                             attccatttcatcggccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGa                                           <  6:438031‑M2/110‑1 (MQ=255)
                                                                                                               tccatttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGAt                                          >  8:1466704‑M2/40‑150 (MQ=255)
                                                                                                                 catttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGt                                       >  8:340388‑M2/38‑151 (MQ=255)
                                                                                                                  atttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGtt                                      >  5:751659‑M2/37‑151 (MQ=255)
                                                                                                                  atttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGtt                                      >  5:367536‑M2/37‑151 (MQ=255)
                                                                                                                  atttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGtt                                      >  3:499998‑M2/37‑151 (MQ=255)
                                                                                                                    ttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGttt                                     <  7:1301013‑M2/116‑1 (MQ=255)
                                                                                                                    ttcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGttt                                     <  4:811744‑M2/116‑1 (MQ=255)
                                                                                                                     tcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCt                                   >  4:1001482‑M2/34‑151 (MQ=255)
                                                                                                                     tcatcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCt                                   >  3:675850‑M2/34‑151 (MQ=255)
                                                                                                                      catcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTg                                  >  8:1246072‑M2/33‑151 (MQ=255)
                                                                                                                      catcgtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTg                                  <  7:1735860‑M2/119‑1 (MQ=255)
                                                                                                                          gtccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCa                              <  2:1060572‑M2/123‑1 (MQ=255)
                                                                                                                           tccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCAt                             >  8:1310814‑M2/28‑151 (MQ=255)
                                                                                                                            ccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATg                            <  5:1884545‑M2/125‑1 (MQ=255)
                                                                                                                            ccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATg                            >  2:66615‑M2/27‑151 (MQ=255)
                                                                                                                            ccaacaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATg                            >  2:495046‑M2/27‑151 (MQ=255)
                                                                                                                             caacaaaattggtgcattacattctTAACTTTCCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATgg                           <  8:1706699‑M2/126‑1 (MQ=255)
                                                                                                                               acaaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGgct                         >  6:1842392‑M2/24‑151 (MQ=255)
                                                                                                                                caaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGgctg                        <  1:1610134‑M2/129‑1 (MQ=255)
                                                                                                                                caaaatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGgctg                        <  3:1194822‑M2/129‑1 (MQ=255)
                                                                                                                                   aatgggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCtt                     >  6:1604745‑M2/20‑151 (MQ=255)
                                                                                                                                      gggtgcagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCtttt                   <  8:644382‑M2/134‑1 (MQ=255)
                                                                                                                                           cagtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCtttt                   <  5:1003722‑M2/134‑1 (MQ=255)
                                                                                                                                             gtacatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCtt           >  4:1747671‑M2/10‑151 (MQ=255)
                                                                                                                                               acatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGATTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTAc         <  5:1989515‑M2/144‑1 (MQ=255)
                                                                                                                                               acatactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTa          <  3:1258240‑M2/143‑1 (MQ=255)
                                                                                                                                                  tactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTc      >  7:712628‑M2/5‑151 (MQ=255)
                                                                                                                                                  tactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTc      >  4:281971‑M2/5‑151 (MQ=255)
                                                                                                                                                  tactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTc      >  7:101394‑M2/5‑151 (MQ=255)
                                                                                                                                                  tactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTc      >  6:663166‑M2/5‑151 (MQ=255)
                                                                                                                                                  tactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTc      >  5:1340919‑M2/5‑151 (MQ=255)
                                                                                                                                                  tactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTc      >  2:1880632‑M2/5‑151 (MQ=255)
                                                                                                                                                  tactGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTc      >  5:17899‑M2/5‑151 (MQ=255)
                                                                                                                                                   actGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTc      <  2:358239‑M2/147‑1 (MQ=255)
                                                                                                                                                    ctGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATAGTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCtt    >  5:954422‑M2/3‑151 (MQ=255)
                                                                                                                                                      gAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTa   <  8:1004270/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GCGCATTTTTCACCATTGCGCTGCTGCGATTCAGGAAGACGATTGGGACAATGGCGTAAATACAATTGCCCGATGCGTAAGCTTATAGGGACTACGCATCGGTGCAATTTATTGAATTTTAGTTGTTTTTAGGCCGGATAAGGCGTTTATGAACTGACCCCCAAAACCTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGTCCTGTATTCAGGGCAAGATTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAA  >  DH1/258026‑258326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: