BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,674,769	Δ12 bp	coding (857‑868/1035 nt)	tqsA →	pheromone AI‑2 transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1674769	1674780	12	414 [0]	[0] 417	tqsA	pheromone AI‑2 transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1674768	0 (0.000)	390 (0.870)	200/288	0.2	100%	coding (856/1035 nt)	tqsA	pheromone AI‑2 transporter
*	?	NC_000913	1674781 =	0 (0.000)	390 (0.870)	200/288	0.2	100%	coding (869/1035 nt)	tqsA	pheromone AI‑2 transporter

Only 100 of 417 total aligned reads displayed.

TTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCG  >  NC_000913/1674632‑1674930

cccgaatattggttcagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatgggggtt                                                                                                                                                      >  2:1173522‑M2/150‑151 (MQ=255)

cccgaatattggttcagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggctt                                                                                                                                                      >  8:1224150‑M2/150‑151 (MQ=255)

 ccgaatattggttcagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcttt                                                                                                                                                     <  2:463587‑M2/3‑1 (MQ=255)

   gaatattggttcagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTcc                                                                                                                                                   <  4:76837‑M2/5‑1 (MQ=255)

   gaatattggttcagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTcc                                                                                                                                                   >  1:437413‑M2/147‑151 (MQ=255)

          ggttcagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcctcggcaatattctggagccgcgtatcatggggcTTTCCACATtgg                                                                                                                                            <  3:401205‑M2/12‑1 (MQ=255)

           gttcagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATtggt                                                                                                                                           >  2:584795‑M2/139‑151 (MQ=255)

             tcagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggctgctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATtggtg                                                                                                                                          <  2:955967‑M2/14‑1 (MQ=255)

              cagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTggtggt                                                                                                                                        <  7:1624978‑M2/16‑1 (MQ=255)

              cagtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTggtggt                                                                                                                                        >  8:1530061‑M2/136‑151 (MQ=255)

               agtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATtggtggtt                                                                                                                                       >  3:1385537‑M2/135‑150 (MQ=255)

                gtcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTAt                                                                                                                                      >  1:370135‑M2/134‑151 (MQ=255)

                 tcctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTAtt                                                                                                                                     >  5:661866‑M2/133‑151 (MQ=255)

                  cctcgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTAttt                                                                                                                                    >  7:1221901‑M2/132‑151 (MQ=255)

                     cgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTAttttt                                                                                                                                  <  3:1560018‑M2/22‑1 (MQ=255)

                     cgcggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTg                                                                                                                                 <  2:453225‑M2/23‑1 (MQ=255)

                       cggcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTc                                                                                                                               <  3:1041416‑M2/25‑1 (MQ=255)

                         gcaatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGt                                                                                                                             >  7:205077‑M2/125‑151 (MQ=255)

                          caatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgatggtgctggcgggatatctgctgattaatctggtcttcggcaatattatggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTTTCGtt                                                                                                                            >  6:520808‑M2/124‑151 (MQ=255)

                           aatcccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTg                                                                                                                           <  3:207925‑M2/29‑1 (MQ=255)

                           aatcccccctatcgctcaggtactggtgattaatggcttttacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTg                                                                                                                           <  2:1430706‑M2/29‑1 (MQ=255)

                              cccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGAt                                                                                                                         <  7:284169‑M2/31‑1 (MQ=255)

                               ccccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTGGTTGAttt                                                                                                                       >  2:751601‑M2/119‑151 (MQ=255)

                                 ccctatcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGAttttt                                                                                                                     <  7:529016‑M2/35‑1 (MQ=255)

                                     atcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTggg                                                                                                                 <  2:515097‑M2/39‑1 (MQ=255)

                                      tcgctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgggg                                                                                                                >  3:70613‑M2/112‑151 (MQ=255)

                                       cgctcagggactggtgtttaatggcttctacgaagcgttgctgctgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGa                                                                                                               <  6:1303619‑M2/41‑1 (MQ=255)

                                         gtcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                                             <  8:1842665‑M2/43‑1 (MQ=255)

                                         ctcaggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                                             >  8:753274‑M2/109‑151 (MQ=255)

                                           caggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg                                                                                                             <  2:1720761‑M2/43‑1 (MQ=255)

                                            aggtactggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgtt                                                                                                          <  1:924641‑M2/46‑1 (MQ=255)

                                                actggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttgtt                                                                                                       >  5:308318‑M2/102‑150 (MQ=255)

                                                actggtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTa                                                                                                      <  5:991943‑M2/50‑1 (MQ=255)

                                                    gtgtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAc                                                                                                  <  8:1183690‑M2/54‑1 (MQ=255)

                                                      gtttaatggcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGAcc                                                                                                 <  5:1185940‑M2/55‑1 (MQ=255)

                                                              gcttctacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAt                                                                                        <  4:1799629‑M2/64‑1 (MQ=255)

                                                                   tacgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTAtgctgc                                                                                   >  5:472669‑M2/83‑151 (MQ=255)

                                                                    acgaagcgttgctggtgctggcgggatatctgctgagtaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttg                                                                                                         >  5:1284219‑M2/82‑128 (MQ=255)

                                                                     cgaagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCtt                                                                                 <  6:1435145‑M2/71‑1 (MQ=255)

                                                                      gaagcgttgctggtgctggctggatatctgctgattaatctggtcttcggcaatattgtggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGTATGGTTGTTAGGACCGGTGGGTATGCTGCttt                                                                                <  6:854287‑M2/72‑1 (MQ=255)

                                                                       aagcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTc                                                                               >  4:929953‑M2/79‑151 (MQ=255)

                                                                        agcgttgctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTcc                                                                              >  6:1166483‑M2/78‑151 (MQ=255)

                                                                              gctggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCg                                                                        <  3:1451334‑M2/80‑1 (MQ=255)

                                                                                tggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                                      >  6:242449‑M2/70‑151 (MQ=255)

                                                                                 tgtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggtgcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGTTTGTTAGGACCGGGGGGTATGCTGCTTTCCGTGCCGtt                                                                      <  5:1534097‑M2/82‑1 (MQ=255)

                                                                                 ggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGtt                                                                      <  2:1493497‑M2/82‑1 (MQ=255)

                                                                                 ggtgctggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGt                                                                       <  1:1794239‑M2/81‑1 (MQ=255)

                                                                                  gtggtggcgggagatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGa                                                                    <  8:294333‑M2/84‑1 (MQ=255)

                                                                                      tggcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTTGTGGTATTTTTGTCGTTGATTTTTTGGGAATGGTTTTAAGAACCGGTGGGTATGATGATTAAAGtgccgttaaaaa                                                                 >  4:1111430‑M2/64‑145 (MQ=255)

                                                                                        gcgggatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatta                                                              <  1:1305425‑M2/90‑1 (MQ=255)

                                                                                        gcgggatatctgctgattaatctgggcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAatta                                                              <  4:1548514‑M2/90‑1 (MQ=255)

                                                                                            gatatctgctgattaatctggtcttcggcaatattctggagccgtgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTTACAATTATTGt                                                          >  4:462097‑M2/58‑151 (MQ=255)

                                                                                            gatatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGt                                                          <  5:1448395‑M2/94‑1 (MQ=255)

                                                                                              tatctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTc                                                         >  1:254690‑M2/56‑150 (MQ=255)

                                                                                               atctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCa                                                        <  3:1726996‑M2/96‑1 (MQ=255)

                                                                                                tctgctgattaatctggtcttcgtcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaa                                                       <  8:896955‑M2/97‑1 (MQ=255)

                                                                                                tctgctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTTTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaa                                                       >  5:445729‑M2/54‑150 (MQ=255)

                                                                                                   gctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCaa                                                       <  8:848431‑M2/97‑1 (MQ=255)

                                                                                                   gctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                                   <  5:1071683‑M2/101‑1 (MQ=255)

                                                                                                   gctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAtt                                                   <  1:119453‑M2/101‑1 (MQ=255)

                                                                                                   gctgattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAAt                                                    <  6:1519089‑M2/100‑1 (MQ=255)

                                                                                                      gattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcg                                                >  1:768095‑M2/48‑151 (MQ=255)

                                                                                                      gattaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTg                                                  <  6:717944‑M2/102‑1 (MQ=255)

                                                                                                       attaatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTgcgc                                               >  3:909157‑M2/47‑151 (MQ=255)

                                                                                                         taatctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCt                                              <  2:371975‑M2/106‑1 (MQ=255)

                                                                                                           atctggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGGTGGTTGTTAGGCCCGGTGGGTATGCTGCTTTCCGTGCCGTTGGCAATTATTGTCAAAATTGCGGTTg                                            >  4:301264‑M2/43‑150 (MQ=255)

                                                                                                             ctggtcttcggcaatattctggagccgcgtatgatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGGTGGTTTCGGTGCCGTTGACAATTATTGTCAAAATTGCGGTTGaac                                         >  2:677171‑M2/41‑151 (MQ=255)

                                                                                                              tggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGTTGGTTGTTAGGACCGGTGGGTATGCTGCGTTCCGTTCCGTTGACAATTATTGTCAAAATTGCGCTTgaacc                                        >  7:1724475‑M2/40‑150 (MQ=255)

                                                                                                               tgtcttcggcaatattctggagccgcgtatcatggggcTTTCCACAATGGTTGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGa                                           <  8:201250‑M2/109‑1 (MQ=255)

                                                                                                               ggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaa                                          <  8:1744469‑M2/110‑1 (MQ=255)

                                                                                                               ggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGa                                           <  1:542143‑M2/109‑1 (MQ=255)

                                                                                                               ggtcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGa                                           <  7:730223‑M2/109‑1 (MQ=255)

                                                                                                                 tcttcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaac                                     >  8:1057766‑M2/37‑151 (MQ=255)

                                                                                                                    tcggcaatattctggtgccgcgtatcatggagcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaaca                                    <  2:246055‑M2/116‑1 (MQ=255)

                                                                                                                    tcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGc                                  >  3:332482‑M2/34‑151 (MQ=255)

                                                                                                                    tcggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTATTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGaacaaaca                                    <  1:1684855‑M2/116‑1 (MQ=255)

                                                                                                                      ggcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCgg                                <  6:1742144‑M2/120‑1 (MQ=255)

                                                                                                                       gcaatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCggg                               >  1:1500223‑M2/31‑151 (MQ=255)

                                                                                                                         aatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCggg                               >  6:450509‑M2/29‑149 (MQ=255)

                                                                                                                         aatattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAg                             <  8:634438‑M2/123‑1 (MQ=255)

                                                                                                                          atattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAgg                            >  5:1846324‑M2/28‑151 (MQ=255)

                                                                                                                          atattctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTAGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTTCCGTTGACAATTATTGTCAAAATTGCGATTGAACAAACAGCTGGAgg                            >  5:1631878‑M2/28‑151 (MQ=255)

                                                                                                                            attctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTc                          <  7:330040‑M2/126‑1 (MQ=255)

                                                                                                                            agtctggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGAGGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTc                          <  6:103298‑M2/126‑1 (MQ=255)

                                                                                                                                tggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCaa                        >  5:787296‑M2/22‑149 (MQ=255)

                                                                                                                                 ggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGTGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAg                      <  4:1300132‑M2/130‑1 (MQ=255)

                                                                                                                                 ggagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCaa                        <  7:831282‑M2/128‑1 (MQ=255)

                                                                                                                                  gagccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCa                    <  8:1695752‑M2/132‑1 (MQ=255)

                                                                                                                                   agccgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCAt                   >  7:1359966‑M2/19‑151 (MQ=255)

                                                                                                                                      cgcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGc                <  6:992060‑M2/136‑1 (MQ=255)

                                                                                                                                       gcgtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGcc               >  2:839053‑M2/15‑151 (MQ=255)

                                                                                                                                         gtatcatggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCAGt             >  5:869879‑M2/13‑151 (MQ=255)

                                                                                                                                             catggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCt          <  8:1338159‑M2/142‑1 (MQ=255)

                                                                                                                                             catggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTg         <  3:102467‑M2/143‑1 (MQ=255)

                                                                                                                                             catggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTAAAAATTGCGCTTGAACAAACAGCGGGAGGACAAAGCATCGCCGTTCTg         >  1:1024056‑M2/9‑151 (MQ=255)

                                                                                                                                                ggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTa      <  3:742287‑M2/146‑1 (MQ=255)

                                                                                                                                                ggggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGGTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGtt       <  2:994021‑M2/145‑1 (MQ=255)

                                                                                                                                                 gggcTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTaa     <  4:378698‑M2/147‑1 (MQ=255)

                                                                                                                                                    gtttCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCg  <  8:342596/150‑1 (MQ=255)

                                                                                                                                                     tttCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCg  >  8:682660/1‑150 (MQ=255)

TTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCG  >  NC_000913/1674632‑1674930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`